Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells Article Swipe
Aline Linder
,
Kevin Portmann
,
Luca Johannes Schlotheuber
,
Alessandro Streuli
,
Wiona Sophie Glänzer
,
Klaus Eyer
,
Ines Lüchtefeld
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3791/66492
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3791/66492
Infections, autoimmune diseases, desired and adverse immunological responses to treatment can lead to a complex and dynamic cytokine response in vivo. This response involves numerous immune cells secreting various cytokines to orchestrate the immune reaction. However, the secretion dynamics, amounts, and co-occurrence of the different cytokines by various cell subtypes remain poorly understood due to a lack of appropriate tools to study them. Here, we describe a protocol using a microfluidic droplet platform that allows the time-resolved quantitative measurement of secretion dynamics for several cytokines in parallel on the single-cell level. This is enabled by the encapsulation of individual cells into microfluidic droplets together with a multiplexed immunoassay for parallel quantification of cytokine concentrations, their immobilization for dynamic fluorescent imaging, and the analysis of the respective images to derive secreted quantities and dynamics. The protocol describes the preparation of functionalized magnetic nanoparticles, calibration experiments, cell preparation, and the encapsulation of the cells and nanoparticles into droplets for fluorescent imaging and subsequent image and data analysis using the example of lipopolysaccharide-stimulated human peripheral blood mononuclear cells. The presented platform identified distinct cytokine secretion behavior for single and co-secreting cells, characterizing the expected phenotypic heterogeneity in the measured cell sample. Furthermore, the modular nature of the assay allows its adaptation and application to study a variety of proteins, cytokines, and cell samples, potentially leading to a deeper understanding of the interplay between different immune cell types and the role of the different cytokines secreted dynamically to shape the tightly regulated immune response. These new insights could be particularly interesting in the studies of immune dysregulations or in identifying target populations in therapy and drug development.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3791/66492
- OA Status
- green
- Cited By
- 5
- References
- 28
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4392755782
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4392755782Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3791/66492Digital Object Identifier
- Title
-
Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional CellsWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-03-08Full publication date if available
- Authors
-
Aline Linder, Kevin Portmann, Luca Johannes Schlotheuber, Alessandro Streuli, Wiona Sophie Glänzer, Klaus Eyer, Ines LüchtefeldList of authors in order
- Landing page
-
https://doi.org/10.3791/66492Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://pure.au.dk/portal/en/publications/17962944-0f4b-4b43-8704-c6fb1afefc23Direct OA link when available
- Concepts
-
Immune system, Cytokine, Secretion, Cell biology, Microfluidics, Cell, Lipopolysaccharide, Biology, Peripheral blood mononuclear cell, Cell type, Computational biology, Immunology, Materials science, Nanotechnology, In vitro, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
5Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 4, 2024: 1Per-year citation counts (last 5 years)
- References (count)
-
28Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4392755782 |
|---|---|
| doi | https://doi.org/10.3791/66492 |
| ids.doi | https://doi.org/10.3929/ethz-b-000666141 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/38526129 |
| ids.openalex | https://openalex.org/W4392755782 |
| fwci | 2.39342266 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D006801 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | Humans |
| mesh[1].qualifier_ui | |
| mesh[1].descriptor_ui | D044085 |
| mesh[1].is_major_topic | True |
| mesh[1].qualifier_name | |
| mesh[1].descriptor_name | Microfluidics |
| mesh[2].qualifier_ui | Q000378 |
| mesh[2].descriptor_ui | D007963 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | metabolism |
| mesh[2].descriptor_name | Leukocytes, Mononuclear |
| mesh[3].qualifier_ui | Q000378 |
| mesh[3].descriptor_ui | D016207 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | metabolism |
| mesh[3].descriptor_name | Cytokines |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D007118 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Immunoassay |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D046210 |
| mesh[5].is_major_topic | True |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Microfluidic Analytical Techniques |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D006801 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Humans |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D044085 |
| mesh[7].is_major_topic | True |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Microfluidics |
| mesh[8].qualifier_ui | Q000378 |
| mesh[8].descriptor_ui | D007963 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | metabolism |
| mesh[8].descriptor_name | Leukocytes, Mononuclear |
| mesh[9].qualifier_ui | Q000378 |
| mesh[9].descriptor_ui | D016207 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | metabolism |
| mesh[9].descriptor_name | Cytokines |
| mesh[10].qualifier_ui | |
| mesh[10].descriptor_ui | D007118 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | |
| mesh[10].descriptor_name | Immunoassay |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D046210 |
| mesh[11].is_major_topic | True |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Microfluidic Analytical Techniques |
| type | article |
| title | Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells |
| biblio.issue | 205 |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T11020 |
| topics[0].field.id | https://openalex.org/fields/24 |
| topics[0].field.display_name | Immunology and Microbiology |
| topics[0].score | 0.9991999864578247 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2403 |
| topics[0].subfield.display_name | Immunology |
| topics[0].display_name | Immune Cell Function and Interaction |
| topics[1].id | https://openalex.org/T11289 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9990000128746033 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Single-cell and spatial transcriptomics |
| topics[2].id | https://openalex.org/T10031 |
| topics[2].field.id | https://openalex.org/fields/24 |
| topics[2].field.display_name | Immunology and Microbiology |
| topics[2].score | 0.9973000288009644 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2403 |
| topics[2].subfield.display_name | Immunology |
| topics[2].display_name | T-cell and B-cell Immunology |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C8891405 |
| concepts[0].level | 2 |
| concepts[0].score | 0.6555784940719604 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q1059 |
| concepts[0].display_name | Immune system |
| concepts[1].id | https://openalex.org/C2778690821 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6461244821548462 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q212354 |
| concepts[1].display_name | Cytokine |
| concepts[2].id | https://openalex.org/C49039625 |
| concepts[2].level | 2 |
| concepts[2].score | 0.6257123947143555 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q84230 |
| concepts[2].display_name | Secretion |
| concepts[3].id | https://openalex.org/C95444343 |
| concepts[3].level | 1 |
| concepts[3].score | 0.5495553612709045 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q7141 |
| concepts[3].display_name | Cell biology |
| concepts[4].id | https://openalex.org/C8673954 |
| concepts[4].level | 2 |
| concepts[4].score | 0.48584815859794617 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q138845 |
| concepts[4].display_name | Microfluidics |
| concepts[5].id | https://openalex.org/C1491633281 |
| concepts[5].level | 2 |
| concepts[5].score | 0.485477477312088 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q7868 |
| concepts[5].display_name | Cell |
| concepts[6].id | https://openalex.org/C2778754761 |
| concepts[6].level | 2 |
| concepts[6].score | 0.4660440981388092 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q421804 |
| concepts[6].display_name | Lipopolysaccharide |
| concepts[7].id | https://openalex.org/C86803240 |
| concepts[7].level | 0 |
| concepts[7].score | 0.4505695104598999 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[7].display_name | Biology |
| concepts[8].id | https://openalex.org/C137061746 |
| concepts[8].level | 3 |
| concepts[8].score | 0.4290539026260376 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q736033 |
| concepts[8].display_name | Peripheral blood mononuclear cell |
| concepts[9].id | https://openalex.org/C189014844 |
| concepts[9].level | 3 |
| concepts[9].score | 0.42197301983833313 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q189118 |
| concepts[9].display_name | Cell type |
| concepts[10].id | https://openalex.org/C70721500 |
| concepts[10].level | 1 |
| concepts[10].score | 0.32016730308532715 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[10].display_name | Computational biology |
| concepts[11].id | https://openalex.org/C203014093 |
| concepts[11].level | 1 |
| concepts[11].score | 0.29813286662101746 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q101929 |
| concepts[11].display_name | Immunology |
| concepts[12].id | https://openalex.org/C192562407 |
| concepts[12].level | 0 |
| concepts[12].score | 0.24085918068885803 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q228736 |
| concepts[12].display_name | Materials science |
| concepts[13].id | https://openalex.org/C171250308 |
| concepts[13].level | 1 |
| concepts[13].score | 0.23571914434432983 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q11468 |
| concepts[13].display_name | Nanotechnology |
| concepts[14].id | https://openalex.org/C202751555 |
| concepts[14].level | 2 |
| concepts[14].score | 0.1649535596370697 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q221681 |
| concepts[14].display_name | In vitro |
| concepts[15].id | https://openalex.org/C55493867 |
| concepts[15].level | 1 |
| concepts[15].score | 0.10739243030548096 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[15].display_name | Biochemistry |
| keywords[0].id | https://openalex.org/keywords/immune-system |
| keywords[0].score | 0.6555784940719604 |
| keywords[0].display_name | Immune system |
| keywords[1].id | https://openalex.org/keywords/cytokine |
| keywords[1].score | 0.6461244821548462 |
| keywords[1].display_name | Cytokine |
| keywords[2].id | https://openalex.org/keywords/secretion |
| keywords[2].score | 0.6257123947143555 |
| keywords[2].display_name | Secretion |
| keywords[3].id | https://openalex.org/keywords/cell-biology |
| keywords[3].score | 0.5495553612709045 |
| keywords[3].display_name | Cell biology |
| keywords[4].id | https://openalex.org/keywords/microfluidics |
| keywords[4].score | 0.48584815859794617 |
| keywords[4].display_name | Microfluidics |
| keywords[5].id | https://openalex.org/keywords/cell |
| keywords[5].score | 0.485477477312088 |
| keywords[5].display_name | Cell |
| keywords[6].id | https://openalex.org/keywords/lipopolysaccharide |
| keywords[6].score | 0.4660440981388092 |
| keywords[6].display_name | Lipopolysaccharide |
| keywords[7].id | https://openalex.org/keywords/biology |
| keywords[7].score | 0.4505695104598999 |
| keywords[7].display_name | Biology |
| keywords[8].id | https://openalex.org/keywords/peripheral-blood-mononuclear-cell |
| keywords[8].score | 0.4290539026260376 |
| keywords[8].display_name | Peripheral blood mononuclear cell |
| keywords[9].id | https://openalex.org/keywords/cell-type |
| keywords[9].score | 0.42197301983833313 |
| keywords[9].display_name | Cell type |
| keywords[10].id | https://openalex.org/keywords/computational-biology |
| keywords[10].score | 0.32016730308532715 |
| keywords[10].display_name | Computational biology |
| keywords[11].id | https://openalex.org/keywords/immunology |
| keywords[11].score | 0.29813286662101746 |
| keywords[11].display_name | Immunology |
| keywords[12].id | https://openalex.org/keywords/materials-science |
| keywords[12].score | 0.24085918068885803 |
| keywords[12].display_name | Materials science |
| keywords[13].id | https://openalex.org/keywords/nanotechnology |
| keywords[13].score | 0.23571914434432983 |
| keywords[13].display_name | Nanotechnology |
| keywords[14].id | https://openalex.org/keywords/in-vitro |
| keywords[14].score | 0.1649535596370697 |
| keywords[14].display_name | In vitro |
| keywords[15].id | https://openalex.org/keywords/biochemistry |
| keywords[15].score | 0.10739243030548096 |
| keywords[15].display_name | Biochemistry |
| language | en |
| locations[0].id | doi:10.3791/66492 |
| locations[0].is_oa | False |
| locations[0].source.id | https://openalex.org/S199738284 |
| locations[0].source.issn | 1940-087X |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 1940-087X |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | Journal of Visualized Experiments |
| locations[0].source.host_organization | https://openalex.org/P4310320416 |
| locations[0].source.host_organization_name | MyJOVE |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320416 |
| locations[0].source.host_organization_lineage_names | MyJOVE |
| locations[0].license | |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Journal of Visualized Experiments |
| locations[0].landing_page_url | https://doi.org/10.3791/66492 |
| locations[1].id | pmid:38526129 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Journal of visualized experiments : JoVE |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/38526129 |
| locations[2].id | pmh:oai:www.research-collection.ethz.ch:20.500.11850/666141 |
| locations[2].is_oa | False |
| locations[2].source.id | https://openalex.org/S4306402302 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | Repository for Publications and Research Data (ETH Zurich) |
| locations[2].source.host_organization | https://openalex.org/I35440088 |
| locations[2].source.host_organization_name | ETH Zurich |
| locations[2].source.host_organization_lineage | https://openalex.org/I35440088 |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | publishedVersion |
| locations[2].raw_type | info:eu-repo/semantics/publishedVersion |
| locations[2].license_id | |
| locations[2].is_accepted | True |
| locations[2].is_published | True |
| locations[2].raw_source_name | Journal of Visualized Experiments. JoVE, 2024 (205) |
| locations[2].landing_page_url | http://hdl.handle.net/20.500.11850/666141 |
| locations[3].id | pmh:oai:pure.atira.dk:publications/17962944-0f4b-4b43-8704-c6fb1afefc23 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400216 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | Research Portal (King's College London) |
| locations[3].source.host_organization | https://openalex.org/I183935753 |
| locations[3].source.host_organization_name | King's College London |
| locations[3].source.host_organization_lineage | https://openalex.org/I183935753 |
| locations[3].license | cc-by |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | article |
| locations[3].license_id | https://openalex.org/licenses/cc-by |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Linder, A, Portmann, K, Schlotheuber, L J, Streuli, A, Glänzer, W S, Eyer, K & Lüchtefeld, I 2024, 'Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells', Journal of Visualized Experiments, vol. 2024, no. 205, e66492. https://doi.org/10.3791/66492 |
| locations[3].landing_page_url | https://pure.au.dk/portal/en/publications/17962944-0f4b-4b43-8704-c6fb1afefc23 |
| locations[4].id | doi:10.3929/ethz-b-000666141 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S7407051236 |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | ETH Zürich Research Collection |
| locations[4].source.host_organization | |
| locations[4].source.host_organization_name | |
| locations[4].license | |
| locations[4].pdf_url | |
| locations[4].version | |
| locations[4].raw_type | article-journal |
| locations[4].license_id | |
| locations[4].is_accepted | False |
| locations[4].is_published | |
| locations[4].raw_source_name | |
| locations[4].landing_page_url | https://doi.org/10.3929/ethz-b-000666141 |
| indexed_in | crossref, datacite, pubmed |
| authorships[0].author.id | https://openalex.org/A5108881054 |
| authorships[0].author.orcid | |
| authorships[0].author.display_name | Aline Linder |
| authorships[0].countries | CH |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[0].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[0].institutions[0].id | https://openalex.org/I35440088 |
| authorships[0].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[0].institutions[0].country_code | CH |
| authorships[0].institutions[0].display_name | ETH Zurich |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Aline Linder |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[1].author.id | https://openalex.org/A5031592028 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Kevin Portmann |
| authorships[1].countries | CH |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[1].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[1].institutions[0].id | https://openalex.org/I35440088 |
| authorships[1].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[1].institutions[0].country_code | CH |
| authorships[1].institutions[0].display_name | ETH Zurich |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Kevin Portmann |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[2].author.id | https://openalex.org/A5093635788 |
| authorships[2].author.orcid | https://orcid.org/0000-0001-8024-1581 |
| authorships[2].author.display_name | Luca Johannes Schlotheuber |
| authorships[2].countries | CH |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[2].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[2].institutions[0].id | https://openalex.org/I35440088 |
| authorships[2].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[2].institutions[0].country_code | CH |
| authorships[2].institutions[0].display_name | ETH Zurich |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Luca Johannes Schlotheuber |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[3].author.id | https://openalex.org/A5042078639 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-0025-8023 |
| authorships[3].author.display_name | Alessandro Streuli |
| authorships[3].countries | CH |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[3].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[3].institutions[0].id | https://openalex.org/I35440088 |
| authorships[3].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[3].institutions[0].country_code | CH |
| authorships[3].institutions[0].display_name | ETH Zurich |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Alessandro Streuli |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[4].author.id | https://openalex.org/A5092772461 |
| authorships[4].author.orcid | |
| authorships[4].author.display_name | Wiona Sophie Glänzer |
| authorships[4].countries | CH |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[4].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[4].institutions[0].id | https://openalex.org/I35440088 |
| authorships[4].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[4].institutions[0].country_code | CH |
| authorships[4].institutions[0].display_name | ETH Zurich |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Wiona Sophie Glänzer |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[5].author.id | https://openalex.org/A5067174905 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-9344-5110 |
| authorships[5].author.display_name | Klaus Eyer |
| authorships[5].countries | CH, DK |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[5].affiliations[0].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[5].affiliations[1].institution_ids | https://openalex.org/I204337017 |
| authorships[5].affiliations[1].raw_affiliation_string | Department of Biomedicine, Aarhus University |
| authorships[5].institutions[0].id | https://openalex.org/I35440088 |
| authorships[5].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[5].institutions[0].country_code | CH |
| authorships[5].institutions[0].display_name | ETH Zurich |
| authorships[5].institutions[1].id | https://openalex.org/I204337017 |
| authorships[5].institutions[1].ror | https://ror.org/01aj84f44 |
| authorships[5].institutions[1].type | education |
| authorships[5].institutions[1].lineage | https://openalex.org/I204337017 |
| authorships[5].institutions[1].country_code | DK |
| authorships[5].institutions[1].display_name | Aarhus University |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Klaus Eyer |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Department of Biomedicine, Aarhus University, Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[6].author.id | https://openalex.org/A5061644431 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-4171-7548 |
| authorships[6].author.display_name | Ines Lüchtefeld |
| authorships[6].countries | CH |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I35440088 |
| authorships[6].affiliations[0].raw_affiliation_string | Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology, ETH Zürich |
| authorships[6].affiliations[1].institution_ids | https://openalex.org/I35440088 |
| authorships[6].affiliations[1].raw_affiliation_string | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich |
| authorships[6].institutions[0].id | https://openalex.org/I35440088 |
| authorships[6].institutions[0].ror | https://ror.org/05a28rw58 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I2799323385, https://openalex.org/I35440088 |
| authorships[6].institutions[0].country_code | CH |
| authorships[6].institutions[0].display_name | ETH Zurich |
| authorships[6].author_position | last |
| authorships[6].raw_author_name | Ines Lüchtefeld |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology, ETH Zürich |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://pure.au.dk/portal/en/publications/17962944-0f4b-4b43-8704-c6fb1afefc23 |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T06:51:31.235846 |
| primary_topic.id | https://openalex.org/T11020 |
| primary_topic.field.id | https://openalex.org/fields/24 |
| primary_topic.field.display_name | Immunology and Microbiology |
| primary_topic.score | 0.9991999864578247 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2403 |
| primary_topic.subfield.display_name | Immunology |
| primary_topic.display_name | Immune Cell Function and Interaction |
| related_works | https://openalex.org/W2087562989, https://openalex.org/W3103016203, https://openalex.org/W2036495427, https://openalex.org/W2073641733, https://openalex.org/W2106238498, https://openalex.org/W2463681241, https://openalex.org/W161520385, https://openalex.org/W4224115270, https://openalex.org/W2065284647, https://openalex.org/W2318823989 |
| cited_by_count | 5 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 4 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 5 |
| best_oa_location.id | pmh:oai:pure.atira.dk:publications/17962944-0f4b-4b43-8704-c6fb1afefc23 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4306400216 |
| best_oa_location.source.issn | |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | Research Portal (King's College London) |
| best_oa_location.source.host_organization | https://openalex.org/I183935753 |
| best_oa_location.source.host_organization_name | King's College London |
| best_oa_location.source.host_organization_lineage | https://openalex.org/I183935753 |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | submittedVersion |
| best_oa_location.raw_type | article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | False |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | Linder, A, Portmann, K, Schlotheuber, L J, Streuli, A, Glänzer, W S, Eyer, K & Lüchtefeld, I 2024, 'Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells', Journal of Visualized Experiments, vol. 2024, no. 205, e66492. https://doi.org/10.3791/66492 |
| best_oa_location.landing_page_url | https://pure.au.dk/portal/en/publications/17962944-0f4b-4b43-8704-c6fb1afefc23 |
| primary_location.id | doi:10.3791/66492 |
| primary_location.is_oa | False |
| primary_location.source.id | https://openalex.org/S199738284 |
| primary_location.source.issn | 1940-087X |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 1940-087X |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | Journal of Visualized Experiments |
| primary_location.source.host_organization | https://openalex.org/P4310320416 |
| primary_location.source.host_organization_name | MyJOVE |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320416 |
| primary_location.source.host_organization_lineage_names | MyJOVE |
| primary_location.license | |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Journal of Visualized Experiments |
| primary_location.landing_page_url | https://doi.org/10.3791/66492 |
| publication_date | 2024-03-08 |
| publication_year | 2024 |
| referenced_works | https://openalex.org/W4365140239, https://openalex.org/W3186424684, https://openalex.org/W3013072344, https://openalex.org/W4387903180, https://openalex.org/W4323810846, https://openalex.org/W3138477144, https://openalex.org/W2885534843, https://openalex.org/W4225547516, https://openalex.org/W4380990320, https://openalex.org/W2076111644, https://openalex.org/W3083950783, https://openalex.org/W2560668196, https://openalex.org/W4385649985, https://openalex.org/W3048393136, https://openalex.org/W3024434553, https://openalex.org/W3170771910, https://openalex.org/W4214627284, https://openalex.org/W3037185429, https://openalex.org/W2589232255, https://openalex.org/W3206604228, https://openalex.org/W1980386568, https://openalex.org/W3084705004, https://openalex.org/W3201791628, https://openalex.org/W3006305829, https://openalex.org/W2750595332, https://openalex.org/W2771472591, https://openalex.org/W4307271407, https://openalex.org/W1568084954 |
| referenced_works_count | 28 |
| abstract_inverted_index.a | 13, 55, 66, 69, 105, 212, 223 |
| abstract_inverted_index.be | 254 |
| abstract_inverted_index.by | 46, 94 |
| abstract_inverted_index.in | 19, 85, 193, 257, 264, 268 |
| abstract_inverted_index.is | 92 |
| abstract_inverted_index.of | 42, 57, 79, 97, 111, 123, 138, 149, 168, 202, 214, 226, 237, 260 |
| abstract_inverted_index.on | 87 |
| abstract_inverted_index.or | 263 |
| abstract_inverted_index.to | 8, 12, 30, 54, 60, 127, 210, 222, 243 |
| abstract_inverted_index.we | 64 |
| abstract_inverted_index.The | 133, 175 |
| abstract_inverted_index.and | 4, 15, 40, 120, 131, 146, 152, 159, 162, 185, 208, 217, 234, 270 |
| abstract_inverted_index.can | 10 |
| abstract_inverted_index.due | 53 |
| abstract_inverted_index.for | 82, 108, 116, 156, 183 |
| abstract_inverted_index.its | 206 |
| abstract_inverted_index.new | 251 |
| abstract_inverted_index.the | 32, 36, 43, 75, 88, 95, 121, 124, 136, 147, 150, 166, 189, 194, 199, 203, 227, 235, 238, 245, 258 |
| abstract_inverted_index.This | 21, 91 |
| abstract_inverted_index.cell | 48, 144, 196, 218, 232 |
| abstract_inverted_index.data | 163 |
| abstract_inverted_index.drug | 271 |
| abstract_inverted_index.into | 100, 154 |
| abstract_inverted_index.lack | 56 |
| abstract_inverted_index.lead | 11 |
| abstract_inverted_index.role | 236 |
| abstract_inverted_index.that | 73 |
| abstract_inverted_index.with | 104 |
| abstract_inverted_index.Here, | 63 |
| abstract_inverted_index.These | 250 |
| abstract_inverted_index.assay | 204 |
| abstract_inverted_index.blood | 172 |
| abstract_inverted_index.cells | 26, 99, 151 |
| abstract_inverted_index.could | 253 |
| abstract_inverted_index.human | 170 |
| abstract_inverted_index.image | 161 |
| abstract_inverted_index.shape | 244 |
| abstract_inverted_index.study | 61, 211 |
| abstract_inverted_index.their | 114 |
| abstract_inverted_index.them. | 62 |
| abstract_inverted_index.tools | 59 |
| abstract_inverted_index.types | 233 |
| abstract_inverted_index.using | 68, 165 |
| abstract_inverted_index.vivo. | 20 |
| abstract_inverted_index.allows | 74, 205 |
| abstract_inverted_index.cells, | 187 |
| abstract_inverted_index.cells. | 174 |
| abstract_inverted_index.deeper | 224 |
| abstract_inverted_index.derive | 128 |
| abstract_inverted_index.images | 126 |
| abstract_inverted_index.immune | 25, 33, 231, 248, 261 |
| abstract_inverted_index.level. | 90 |
| abstract_inverted_index.nature | 201 |
| abstract_inverted_index.poorly | 51 |
| abstract_inverted_index.remain | 50 |
| abstract_inverted_index.single | 184 |
| abstract_inverted_index.target | 266 |
| abstract_inverted_index.adverse | 5 |
| abstract_inverted_index.between | 229 |
| abstract_inverted_index.complex | 14 |
| abstract_inverted_index.desired | 3 |
| abstract_inverted_index.droplet | 71 |
| abstract_inverted_index.dynamic | 16, 117 |
| abstract_inverted_index.enabled | 93 |
| abstract_inverted_index.example | 167 |
| abstract_inverted_index.imaging | 158 |
| abstract_inverted_index.leading | 221 |
| abstract_inverted_index.modular | 200 |
| abstract_inverted_index.sample. | 197 |
| abstract_inverted_index.several | 83 |
| abstract_inverted_index.studies | 259 |
| abstract_inverted_index.therapy | 269 |
| abstract_inverted_index.tightly | 246 |
| abstract_inverted_index.variety | 213 |
| abstract_inverted_index.various | 28, 47 |
| abstract_inverted_index.However, | 35 |
| abstract_inverted_index.amounts, | 39 |
| abstract_inverted_index.analysis | 122, 164 |
| abstract_inverted_index.behavior | 182 |
| abstract_inverted_index.cytokine | 17, 112, 180 |
| abstract_inverted_index.describe | 65 |
| abstract_inverted_index.distinct | 179 |
| abstract_inverted_index.droplets | 102, 155 |
| abstract_inverted_index.dynamics | 81 |
| abstract_inverted_index.expected | 190 |
| abstract_inverted_index.imaging, | 119 |
| abstract_inverted_index.insights | 252 |
| abstract_inverted_index.involves | 23 |
| abstract_inverted_index.magnetic | 140 |
| abstract_inverted_index.measured | 195 |
| abstract_inverted_index.numerous | 24 |
| abstract_inverted_index.parallel | 86, 109 |
| abstract_inverted_index.platform | 72, 177 |
| abstract_inverted_index.protocol | 67, 134 |
| abstract_inverted_index.response | 18, 22 |
| abstract_inverted_index.samples, | 219 |
| abstract_inverted_index.secreted | 129, 241 |
| abstract_inverted_index.subtypes | 49 |
| abstract_inverted_index.together | 103 |
| abstract_inverted_index.cytokines | 29, 45, 84, 240 |
| abstract_inverted_index.describes | 135 |
| abstract_inverted_index.different | 44, 230, 239 |
| abstract_inverted_index.diseases, | 2 |
| abstract_inverted_index.dynamics, | 38 |
| abstract_inverted_index.dynamics. | 132 |
| abstract_inverted_index.interplay | 228 |
| abstract_inverted_index.presented | 176 |
| abstract_inverted_index.proteins, | 215 |
| abstract_inverted_index.reaction. | 34 |
| abstract_inverted_index.regulated | 247 |
| abstract_inverted_index.response. | 249 |
| abstract_inverted_index.responses | 7 |
| abstract_inverted_index.secreting | 27 |
| abstract_inverted_index.secretion | 37, 80, 181 |
| abstract_inverted_index.treatment | 9 |
| abstract_inverted_index.adaptation | 207 |
| abstract_inverted_index.autoimmune | 1 |
| abstract_inverted_index.cytokines, | 216 |
| abstract_inverted_index.identified | 178 |
| abstract_inverted_index.individual | 98 |
| abstract_inverted_index.peripheral | 171 |
| abstract_inverted_index.phenotypic | 191 |
| abstract_inverted_index.quantities | 130 |
| abstract_inverted_index.respective | 125 |
| abstract_inverted_index.subsequent | 160 |
| abstract_inverted_index.understood | 52 |
| abstract_inverted_index.Infections, | 0 |
| abstract_inverted_index.application | 209 |
| abstract_inverted_index.appropriate | 58 |
| abstract_inverted_index.calibration | 142 |
| abstract_inverted_index.dynamically | 242 |
| abstract_inverted_index.fluorescent | 118, 157 |
| abstract_inverted_index.identifying | 265 |
| abstract_inverted_index.immunoassay | 107 |
| abstract_inverted_index.interesting | 256 |
| abstract_inverted_index.measurement | 78 |
| abstract_inverted_index.mononuclear | 173 |
| abstract_inverted_index.multiplexed | 106 |
| abstract_inverted_index.orchestrate | 31 |
| abstract_inverted_index.populations | 267 |
| abstract_inverted_index.potentially | 220 |
| abstract_inverted_index.preparation | 137 |
| abstract_inverted_index.single-cell | 89 |
| abstract_inverted_index.Furthermore, | 198 |
| abstract_inverted_index.co-secreting | 186 |
| abstract_inverted_index.development. | 272 |
| abstract_inverted_index.experiments, | 143 |
| abstract_inverted_index.microfluidic | 70, 101 |
| abstract_inverted_index.particularly | 255 |
| abstract_inverted_index.preparation, | 145 |
| abstract_inverted_index.quantitative | 77 |
| abstract_inverted_index.co-occurrence | 41 |
| abstract_inverted_index.encapsulation | 96, 148 |
| abstract_inverted_index.heterogeneity | 192 |
| abstract_inverted_index.immunological | 6 |
| abstract_inverted_index.nanoparticles | 153 |
| abstract_inverted_index.time-resolved | 76 |
| abstract_inverted_index.understanding | 225 |
| abstract_inverted_index.characterizing | 188 |
| abstract_inverted_index.dysregulations | 262 |
| abstract_inverted_index.functionalized | 139 |
| abstract_inverted_index.immobilization | 115 |
| abstract_inverted_index.nanoparticles, | 141 |
| abstract_inverted_index.quantification | 110 |
| abstract_inverted_index.concentrations, | 113 |
| abstract_inverted_index.lipopolysaccharide-stimulated | 169 |
| cited_by_percentile_year.max | 98 |
| cited_by_percentile_year.min | 90 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 7 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/3 |
| sustainable_development_goals[0].score | 0.8399999737739563 |
| sustainable_development_goals[0].display_name | Good health and well-being |
| citation_normalized_percentile.value | 0.8259171 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |