MINFLUX dissects nucleosome and compacting chromatin structures in living cells Article Swipe
Hong Xie
,
Yi Hu
,
Rui Cheng
,
Xiaohui He
,
Kaiyuan Liu
,
Shaoqian Zhang
,
Bin Xie
,
Fang He
,
Ying Yang
,
Lei Xu
,
Xi Wang
,
Jian Lin
,
Guohong Li
,
Ji‐Song Guan
,
Hanhui Ma
,
Miṅ Gu
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/nsr/nwaf451
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/nsr/nwaf451
The chromatin structure is fundamental for genome compaction and gene transcriptional regulation in nucleus. Although in vitro studies suggest a classical model that 11-nm nucleosome polymers fold into 30 nm fibers, such structures have not been observed in situ. In contrast, disordered chains or condensed liquid-like chromatin domains are reported in cells by EM studies and by super-resolution fluorescence microscopy. Do condensed chromatin fibers indeed exist in the cell? We identified a florescent dye that preferentially binds to AT-rich regions of DNA and blinks spontaneously to allow single probe visualization. Using three-dimensional MINFLUX localization, which is extreme low phototoxicity and high-speed, we observed that a subset of DNA molecules assemble into fiber-like structures co-localizated with histones. Native chromatin fibers in living cells are detected in the middle of nucleus with segments variable in width. Most of chromatin fibers are dismissed after Trichostatin A (TSA) treatment. In some cases, DNA localization even reveals the 3D ultrastructure of individual nucleosomes as 5-10 probes wrapping around a 11-nm cylinder in living cells. Therefore, chromatin fibers (∼30 nm) do exist in living cells, at least in AT-rich regions of the genome. The MINFLUX nanoscopy reveals native chromatin ultrastructure and its folding to achieve structural compaction in nucleus.
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- Language
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- Landing Page
- https://doi.org/10.1093/nsr/nwaf451
- OA Status
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- OpenAlex ID
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- Title
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MINFLUX dissects nucleosome and compacting chromatin structures in living cellsWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-10-20Full publication date if available
- Authors
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Hong Xie, Yi Hu, Rui Cheng, Xiaohui He, Kaiyuan Liu, Shaoqian Zhang, Bin Xie, Fang He, Ying Yang, Lei Xu, Xi Wang, Jian Lin, Guohong Li, Ji‐Song Guan, Hanhui Ma, Miṅ GuList of authors in order
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https://doi.org/10.1093/nsr/nwaf451Publisher landing page
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goldOpen access status per OpenAlex
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https://doi.org/10.1093/nsr/nwaf451Direct OA link when available
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