Modular Cloning of Multigene Vectors for the Baculovirus System and Yeast Article Swipe
Zhihao Lai
,
Sarena F. Flanigan
,
Marion Boudes
,
Chen Davidovich
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.jmb.2025.168943
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.jmb.2025.168943
Recombinant macromolecular complexes are often produced by the baculovirus system, using multigene expression vectors. Yet, the construction of baculovirus-compatible multigene expression vectors is complicated and time-consuming. Furthermore, while the baculovirus and yeast are popular protein expression systems, no single method for multigene vector construction is compatible with both. Here we present the modular cloning (MoClo) Baculo toolkit for constructing multigene expression vectors for the baculovirus system and, through compatibility with the MoClo Yeast toolkit, also for yeast. Vector construction by MoClo Baculo utilises Golden Gate assembly, which does not require PCR, primers or the sequencing of intermediate products. As a proof of principle, MoClo Baculo was used to construct baculovirus and yeast multigene vectors expressing the four- and five-subunit human Polycomb Repressive Complex 2. We show that MoClo Baculo simplifies and expedites the construction of multigene expression vectors for the baculovirus system and provides compatibility with yeast as an alternative expression system.
Related Topics
Concepts
Cloning (programming)
Yeast
Biology
Modular design
Computational biology
Genetics
Vector (molecular biology)
Microbiology
Virology
Recombinant DNA
Gene
Computer science
Programming language
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.jmb.2025.168943
- OA Status
- hybrid
- Cited By
- 5
- References
- 29
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
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https://openalex.org/W4406315560Canonical identifier for this work in OpenAlex
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https://doi.org/10.1016/j.jmb.2025.168943Digital Object Identifier
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Modular Cloning of Multigene Vectors for the Baculovirus System and YeastWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-01-13Full publication date if available
- Authors
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Zhihao Lai, Sarena F. Flanigan, Marion Boudes, Chen DavidovichList of authors in order
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https://doi.org/10.1016/j.jmb.2025.168943Publisher landing page
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://doi.org/10.1016/j.jmb.2025.168943Direct OA link when available
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Cloning (programming), Yeast, Biology, Modular design, Computational biology, Genetics, Vector (molecular biology), Microbiology, Virology, Recombinant DNA, Gene, Computer science, Programming languageTop concepts (fields/topics) attached by OpenAlex
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5Total citation count in OpenAlex
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2025: 5Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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