Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis Article Swipe
Antonio Calabuig‐Serna
,
Daniel Sancho‐Oviedo
,
Alba Rivas-Sendra
,
Estefanía Mata-Nicolás
,
Paloma Arjona‐Mudarra
,
Ricardo Mir
,
José M. Seguí‐Simarro
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/aob/mcaf054
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1093/aob/mcaf054
Background and Aims Microspore embryogenesis is a convenient inducible system to study the changes associated with the developmental reprogramming of cells. In this work, Brassica napus microspore cultures were used to study the role in the embryogenic switch of callose and pectin cell wall composition, which depends on Ca2+ levels. Methods We used different chemicals to modulate Ca2+, callose and pectin methyl-esterification, including Ca(NO3)2, inositol 1,4,5-trisphosphate, 2-deoxy-d-glucose, benzyl alcohol, chitosan, epigallocatechin gallate and pectin methyl-esterase. Ca2+ distribution, callose and cellulose were imaged with FluoForte, Aniline Blue and Pontamine Fast Scarlet stainings, respectively, and observed with confocal microscopy. Key Results Inhibition of callose synthesis with 2-deoxy-d-glucose demonstrated that callose is essential for induction of microspore embryogenesis. A moderate increase of Ca2+ levels with Ca(NO3)2 or inositol 1,4,5-trisphosphate promoted increased callose synthesis and deposition in the cell wall. However, the use of benzyl alcohol and chitosan to permeabilize the plasma membrane and allow for Ca2+ influx was not positive, because this prevented embryo development by inducing callus formation. Benzyl alcohol did not affect callose and cellulose deposition, but chitosan induced the formation of callose plugs, similar to those formed in response to pathogen attack. Inhibition of pectin methyl-esterase activity with epigallocatechin gallate during the first 3 days of culture produced ~70 % more embryos, but prolonged exposures were negative. Instead, increased pectin methyl-esterase activity during the first 3 days was not positive, but when applied for 7 days, embryos increased by ~60 %. Conclusions Together, these results confirm the relevant role of calcium and callose during the first stages of microspore induction and suggest that the levels of pectin methyl-esterification in the cell wall are dynamic and that different cell wall compositions are required during the different stages of microspore embryogenesis.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1093/aob/mcaf054
- https://academic.oup.com/aob/advance-article-pdf/doi/10.1093/aob/mcaf054/62927528/mcaf054.pdf
- OA Status
- hybrid
- Cited By
- 1
- References
- 49
- Related Works
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- OpenAlex ID
- https://openalex.org/W4409469255
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4409469255Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1093/aob/mcaf054Digital Object Identifier
- Title
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Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesisWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-04-15Full publication date if available
- Authors
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Antonio Calabuig‐Serna, Daniel Sancho‐Oviedo, Alba Rivas-Sendra, Estefanía Mata-Nicolás, Paloma Arjona‐Mudarra, Ricardo Mir, José M. Seguí‐SimarroList of authors in order
- Landing page
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https://doi.org/10.1093/aob/mcaf054Publisher landing page
- PDF URL
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https://academic.oup.com/aob/advance-article-pdf/doi/10.1093/aob/mcaf054/62927528/mcaf054.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
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hybridOpen access status per OpenAlex
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https://academic.oup.com/aob/advance-article-pdf/doi/10.1093/aob/mcaf054/62927528/mcaf054.pdfDirect OA link when available
- Concepts
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Callose, Biology, Microspore, Biochemistry, Polysaccharide, Cell wall, Cell biology, Botany, Biophysics, Pollen, StamenTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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49Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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