MOL-PCR and xMAP Technology Article Swipe
Jakub Hrdý
,
Petra Vašíčková
,
Michaela Nesvadbová
,
Jiřı́ Novotný
,
Tomas Mati
,
Petr Králík
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1016/j.jmoldx.2021.03.005
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1016/j.jmoldx.2021.03.005
Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. A new semiquantitative magnetic bead-based multiplex system has been designed for simultaneous detection of several targets in one reaction. The system includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV), and a target for external control of the system. To evaluate the detection system, interlaboratory ring tests were performed in four independent laboratories. Analytical specificity of the tool was tested on a cohort of pathogenic agents and biological samples with quantitative PCR as a reference method. Limit of detection (analytical sensitivity) of 5 × 100 (AdV, HEV, and RVA) and 5 × 101 (HAV and NoV) genome equivalents per reaction was reached. This robust, senstivie, and rapid multiplexing technology may be used to routinely monitor and manage viruses in food and water to prevent food and waterborne diseases.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.jmoldx.2021.03.005
- http://www.jmdjournal.org/article/S1525157821000866/pdf
- OA Status
- hybrid
- Cited By
- 8
- References
- 52
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3153783311
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3153783311Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.jmoldx.2021.03.005Digital Object Identifier
- Title
-
MOL-PCR and xMAP TechnologyWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2021Year of publication
- Publication date
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2021-04-15Full publication date if available
- Authors
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Jakub Hrdý, Petra Vašíčková, Michaela Nesvadbová, Jiřı́ Novotný, Tomas Mati, Petr KrálíkList of authors in order
- Landing page
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https://doi.org/10.1016/j.jmoldx.2021.03.005Publisher landing page
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https://www.jmdjournal.org/article/S1525157821000866/pdfDirect link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://www.jmdjournal.org/article/S1525157821000866/pdfDirect OA link when available
- Concepts
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Norovirus, Rotavirus, Multiplex, Hepatitis a virus, Virology, Biology, Detection limit, Food safety, Magnetic bead, Virus, Microbiology, Food science, Chromatography, Bioinformatics, ChemistryTop concepts (fields/topics) attached by OpenAlex
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8Total citation count in OpenAlex
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2025: 1, 2023: 3, 2022: 3, 2021: 1Per-year citation counts (last 5 years)
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52Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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