Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Assam, India Article Swipe
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· 2022
· Open Access
·
· DOI: https://doi.org/10.21887/ijvsbt.18.3.22
The present study was carried out to detect the circulating Newcastle disease virus (NDV) from various field outbreaks in Assam during 2018-2021. Nine hundred ninety-two clinical and post mortem samples were collected from the backyard and commercial poultry and subjected to molecular detection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected in 445 (n=757, 58.78%) tissue samples and 53 (n=235, 22.55%) cloacal swabs collected from suspected poultry cases by RT-PCR. The findings of our study suggests that the sample of choice for detection of NDV from tissue samples was trachea (n=85, 92.94%), followed by spleen (n=96, 90.62%), lung (n=92, 86.95%), caecal tonsils (n=96, 77.08%), proventriculus (n=74, 60.81%), brain (n=92, 48.91%), intestines (n=73, 31.50%), kidney (n=52, 23.07%), and cloacal swab (n=235, 22.55%) by RT-PCR. Hence, the collection of specific tissue samples from field outbreaks for the detection of NDV is of paramount importance. In addition, RT-PCR can be considered a highly sensitive detection method for rapid and confirmative diagnosis of Newcastle disease virus.
Related Topics
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.21887/ijvsbt.18.3.22
- https://acspublisher.com/journals/index.php/ijvsbt/article/download/2238/2172
- OA Status
- hybrid
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- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W4289891048Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21887/ijvsbt.18.3.22Digital Object Identifier
- Title
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Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Assam, IndiaWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-07-29Full publication date if available
- Authors
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Pubaleem Deka, Sangeeta Das, Pankaj Deka, Mrinal Kumar Nath, Ritam Hazarika, Gunajit Das, Mihir SarmaList of authors in order
- Landing page
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https://doi.org/10.21887/ijvsbt.18.3.22Publisher landing page
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https://acspublisher.com/journals/index.php/ijvsbt/article/download/2238/2172Direct link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://acspublisher.com/journals/index.php/ijvsbt/article/download/2238/2172Direct OA link when available
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Newcastle disease, Outbreak, Virus, Proventriculus, Biology, Virology, Polymerase chain reaction, Veterinary medicine, Medicine, Anatomy, Biochemistry, GeneTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2025: 1, 2024: 2Per-year citation counts (last 5 years)
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21Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.commercial | 36 |
| abstract_inverted_index.considered | 149 |
| abstract_inverted_index.intestines | 112 |
| abstract_inverted_index.ninety-two | 24 |
| abstract_inverted_index.circulating | 9 |
| abstract_inverted_index.importance. | 143 |
| abstract_inverted_index.confirmative | 158 |
| abstract_inverted_index.proventriculus | 106 |
| abstract_inverted_index.Transcriptase-Polymerase | 45 |
| cited_by_percentile_year.max | 96 |
| cited_by_percentile_year.min | 91 |
| countries_distinct_count | 0 |
| institutions_distinct_count | 7 |
| citation_normalized_percentile.value | 0.64981049 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |