Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic Lung Article Swipe
Yi-Chien Wu
,
Hyung‐Geun Moon
,
Vytautas P. Bindokas
,
Evan H. Phillips
,
Gye Young Park
,
Seung Young Lee
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1165/rcmb.2022-0353ma
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1165/rcmb.2022-0353ma
Asthma is a chronic inflammatory airway disease driven by various infiltrating immune cell types into the lung. Optical microscopy has been used to study immune infiltrates in asthmatic lungs. Confocal laser scanning microscopy (CLSM) identifies the phenotypes and locations of individual immune cells in lung tissue sections by employing high-magnification objectives and multiplex immunofluorescence staining. In contrast, light-sheet fluorescence microscopy (LSFM) can visualize the macroscopic and mesoscopic architecture of whole-mount lung tissues in three dimensions (3D) by adopting an optical tissue-clearing method. Despite each microscopy method producing image data with unique resolution from a tissue sample, CLSM and LSFM have not been applied together because of different tissue-preparation procedures. Here, we introduce a new approach combining LSFM and CLSM into a sequential imaging pipeline. We built a new optical tissue clearing workflow in which the immersion clearing agent can be switched from an organic solvent to an aqueous sugar solution for sequential 3D LSFM and CLSM of mouse lungs. This sequential combination microscopy offered quantitative 3D spatial analyses of the distribution of immune infiltrates in the same mouse asthmatic lung tissue at the organ, tissue, and cell levels. These results show that our method facilitates multiresolution 3D fluorescence microscopy as a new imaging approach providing comprehensive spatial information for a better understanding of inflammatory lung diseases.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1165/rcmb.2022-0353ma
- OA Status
- hybrid
- Cited By
- 10
- References
- 38
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4362602240
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4362602240Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1165/rcmb.2022-0353maDigital Object Identifier
- Title
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Multiresolution 3D Optical Mapping of Immune Cell Infiltrates in Mouse Asthmatic LungWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-04-05Full publication date if available
- Authors
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Yi-Chien Wu, Hyung‐Geun Moon, Vytautas P. Bindokas, Evan H. Phillips, Gye Young Park, Seung Young LeeList of authors in order
- Landing page
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https://doi.org/10.1165/rcmb.2022-0353maPublisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1165/rcmb.2022-0353maDirect OA link when available
- Concepts
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Immune system, Lung, Immunology, Pathology, Cell, Biology, Medicine, Computational biology, Genetics, Internal medicineTop concepts (fields/topics) attached by OpenAlex
- Cited by
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10Total citation count in OpenAlex
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2025: 5, 2024: 3, 2023: 2Per-year citation counts (last 5 years)
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38Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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