Mutual stabilization of detergent resistant subtilisin and heat stable streptomyces subtilisin inhibitor by complex formation. Article Swipe
Tsutomu Arakawa
,
Kelly Lazenby
,
Carl G. Kolvenbach
,
T. F. Moran
,
Michael McGinley
,
Michael F. Rohde
,
Linda O. Narhi
·
YOU?
·
· 1991
· Open Access
·
· DOI: https://doi.org/10.1271/bbb1961.55.903
YOU?
·
· 1991
· Open Access
·
· DOI: https://doi.org/10.1271/bbb1961.55.903
The interaction between subtilisin (Sbt) and Streptomyces subtilisin inhibitor (SSI) was examined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). When a mixture of Sbt and SSI was incubated together and then mixed with SDS-PAGE sample buffer at room temperature, it had abnormal behavior on SDS-PAGE; a single band corresponding to the complex migrated only to the top of the gel. Sequence analysis of the band electroblotted from the gel showed two major N-terminal sequences corresponding to Sbt and SSI. When the same Sbt/SSI mixture was analyzed by reverse phase-high performance liquid chromatography, protein eluted in positions corresponding to the intact forms of Sbt and SSI. Thus, it was concluded that the Sbt/SSI complex is stable upon exposure to SDS and migrates abnormally as a complex during SDS-PAGE; the abnormal migration stems from the inherent resistance of Sbt to SDS. The denaturation of the Sbt/SSI complex was examined by heating it at different temperatures, rapid cooling, and SDS-PAGE. The band corresponding to the complex gradually disappeared as the temperature of the complex was increased from 70°C to 85°C; from this, the transition temperature was about 78°C. This temperature is higher than the known transition temperature of the Sbt alone (60°C to 65°C), indicating stabilization of the Sbt by SSI. This transition was accompanied by the appearance of low molecular weight species, none of which corresponded to the intact sizes of Sbt or SSI, indicating that autolysis occurred following denaturation.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1271/bbb1961.55.903
- https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdf
- OA Status
- bronze
- References
- 1
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W1964517479
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W1964517479Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1271/bbb1961.55.903Digital Object Identifier
- Title
-
Mutual stabilization of detergent resistant subtilisin and heat stable streptomyces subtilisin inhibitor by complex formation.Work title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
1991Year of publication
- Publication date
-
1991-01-01Full publication date if available
- Authors
-
Tsutomu Arakawa, Kelly Lazenby, Carl G. Kolvenbach, T. F. Moran, Michael McGinley, Michael F. Rohde, Linda O. NarhiList of authors in order
- Landing page
-
https://doi.org/10.1271/bbb1961.55.903Publisher landing page
- PDF URL
-
https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
bronzeOpen access status per OpenAlex
- OA URL
-
https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdfDirect OA link when available
- Concepts
-
Subtilisin, Chemistry, Denaturation (fissile materials), Autolysis (biology), Gel electrophoresis, Chromatography, Sodium dodecyl sulfate, Polyacrylamide gel electrophoresis, Electrophoresis, Sodium, Biochemistry, Nuclear chemistry, Enzyme, Organic chemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- References (count)
-
1Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W1964517479 |
|---|---|
| doi | https://doi.org/10.1271/bbb1961.55.903 |
| ids.doi | https://doi.org/10.1271/bbb1961.55.903 |
| ids.mag | 1964517479 |
| ids.openalex | https://openalex.org/W1964517479 |
| fwci | 0.0 |
| type | article |
| title | Mutual stabilization of detergent resistant subtilisin and heat stable streptomyces subtilisin inhibitor by complex formation. |
| biblio.issue | 4 |
| biblio.volume | 55 |
| biblio.last_page | 910 |
| biblio.first_page | 903 |
| topics[0].id | https://openalex.org/T11617 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9872999787330627 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1305 |
| topics[0].subfield.display_name | Biotechnology |
| topics[0].display_name | Enzyme Production and Characterization |
| topics[1].id | https://openalex.org/T11162 |
| topics[1].field.id | https://openalex.org/fields/25 |
| topics[1].field.display_name | Materials Science |
| topics[1].score | 0.9847999811172485 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2505 |
| topics[1].subfield.display_name | Materials Chemistry |
| topics[1].display_name | Enzyme Structure and Function |
| topics[2].id | https://openalex.org/T11124 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9556000232696533 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1312 |
| topics[2].subfield.display_name | Molecular Biology |
| topics[2].display_name | Protein purification and stability |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C2779811187 |
| concepts[0].level | 3 |
| concepts[0].score | 0.9363980293273926 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q423655 |
| concepts[0].display_name | Subtilisin |
| concepts[1].id | https://openalex.org/C185592680 |
| concepts[1].level | 0 |
| concepts[1].score | 0.8259477019309998 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[1].display_name | Chemistry |
| concepts[2].id | https://openalex.org/C2776923230 |
| concepts[2].level | 2 |
| concepts[2].score | 0.7283146381378174 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q16955798 |
| concepts[2].display_name | Denaturation (fissile materials) |
| concepts[3].id | https://openalex.org/C104810894 |
| concepts[3].level | 3 |
| concepts[3].score | 0.6898980140686035 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q787099 |
| concepts[3].display_name | Autolysis (biology) |
| concepts[4].id | https://openalex.org/C116084860 |
| concepts[4].level | 2 |
| concepts[4].score | 0.6297805905342102 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q48255 |
| concepts[4].display_name | Gel electrophoresis |
| concepts[5].id | https://openalex.org/C43617362 |
| concepts[5].level | 1 |
| concepts[5].score | 0.6297447681427002 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q170050 |
| concepts[5].display_name | Chromatography |
| concepts[6].id | https://openalex.org/C2778913249 |
| concepts[6].level | 2 |
| concepts[6].score | 0.5530041456222534 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q422241 |
| concepts[6].display_name | Sodium dodecyl sulfate |
| concepts[7].id | https://openalex.org/C101660505 |
| concepts[7].level | 3 |
| concepts[7].score | 0.5435686707496643 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q898301 |
| concepts[7].display_name | Polyacrylamide gel electrophoresis |
| concepts[8].id | https://openalex.org/C40250595 |
| concepts[8].level | 2 |
| concepts[8].score | 0.4266957640647888 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q185098 |
| concepts[8].display_name | Electrophoresis |
| concepts[9].id | https://openalex.org/C537181965 |
| concepts[9].level | 2 |
| concepts[9].score | 0.4242621958255768 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q658 |
| concepts[9].display_name | Sodium |
| concepts[10].id | https://openalex.org/C55493867 |
| concepts[10].level | 1 |
| concepts[10].score | 0.24834507703781128 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[10].display_name | Biochemistry |
| concepts[11].id | https://openalex.org/C13965031 |
| concepts[11].level | 1 |
| concepts[11].score | 0.14256447553634644 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q243545 |
| concepts[11].display_name | Nuclear chemistry |
| concepts[12].id | https://openalex.org/C181199279 |
| concepts[12].level | 2 |
| concepts[12].score | 0.11998218297958374 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q8047 |
| concepts[12].display_name | Enzyme |
| concepts[13].id | https://openalex.org/C178790620 |
| concepts[13].level | 1 |
| concepts[13].score | 0.10762536525726318 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q11351 |
| concepts[13].display_name | Organic chemistry |
| keywords[0].id | https://openalex.org/keywords/subtilisin |
| keywords[0].score | 0.9363980293273926 |
| keywords[0].display_name | Subtilisin |
| keywords[1].id | https://openalex.org/keywords/chemistry |
| keywords[1].score | 0.8259477019309998 |
| keywords[1].display_name | Chemistry |
| keywords[2].id | https://openalex.org/keywords/denaturation |
| keywords[2].score | 0.7283146381378174 |
| keywords[2].display_name | Denaturation (fissile materials) |
| keywords[3].id | https://openalex.org/keywords/autolysis |
| keywords[3].score | 0.6898980140686035 |
| keywords[3].display_name | Autolysis (biology) |
| keywords[4].id | https://openalex.org/keywords/gel-electrophoresis |
| keywords[4].score | 0.6297805905342102 |
| keywords[4].display_name | Gel electrophoresis |
| keywords[5].id | https://openalex.org/keywords/chromatography |
| keywords[5].score | 0.6297447681427002 |
| keywords[5].display_name | Chromatography |
| keywords[6].id | https://openalex.org/keywords/sodium-dodecyl-sulfate |
| keywords[6].score | 0.5530041456222534 |
| keywords[6].display_name | Sodium dodecyl sulfate |
| keywords[7].id | https://openalex.org/keywords/polyacrylamide-gel-electrophoresis |
| keywords[7].score | 0.5435686707496643 |
| keywords[7].display_name | Polyacrylamide gel electrophoresis |
| keywords[8].id | https://openalex.org/keywords/electrophoresis |
| keywords[8].score | 0.4266957640647888 |
| keywords[8].display_name | Electrophoresis |
| keywords[9].id | https://openalex.org/keywords/sodium |
| keywords[9].score | 0.4242621958255768 |
| keywords[9].display_name | Sodium |
| keywords[10].id | https://openalex.org/keywords/biochemistry |
| keywords[10].score | 0.24834507703781128 |
| keywords[10].display_name | Biochemistry |
| keywords[11].id | https://openalex.org/keywords/nuclear-chemistry |
| keywords[11].score | 0.14256447553634644 |
| keywords[11].display_name | Nuclear chemistry |
| keywords[12].id | https://openalex.org/keywords/enzyme |
| keywords[12].score | 0.11998218297958374 |
| keywords[12].display_name | Enzyme |
| keywords[13].id | https://openalex.org/keywords/organic-chemistry |
| keywords[13].score | 0.10762536525726318 |
| keywords[13].display_name | Organic chemistry |
| language | en |
| locations[0].id | doi:10.1271/bbb1961.55.903 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2764346059 |
| locations[0].source.issn | 0002-1369, 1881-1280 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 0002-1369 |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | Agricultural and Biological Chemistry |
| locations[0].source.host_organization | https://openalex.org/P4310311648 |
| locations[0].source.host_organization_name | Oxford University Press |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310311648 |
| locations[0].license | |
| locations[0].pdf_url | https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Agricultural and Biological Chemistry |
| locations[0].landing_page_url | https://doi.org/10.1271/bbb1961.55.903 |
| indexed_in | crossref |
| authorships[0].author.id | https://openalex.org/A5035285523 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-7145-012X |
| authorships[0].author.display_name | Tsutomu Arakawa |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[0].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[0].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[0].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[0].institutions[0].type | company |
| authorships[0].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | Amgen (United States) |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Tsutomu ARAKAWA |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[1].author.id | https://openalex.org/A5087077601 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Kelly Lazenby |
| authorships[1].countries | US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[1].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[1].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[1].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[1].institutions[0].type | company |
| authorships[1].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[1].institutions[0].country_code | US |
| authorships[1].institutions[0].display_name | Amgen (United States) |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Kelly LAZENBY |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[2].author.id | https://openalex.org/A5019989148 |
| authorships[2].author.orcid | |
| authorships[2].author.display_name | Carl G. Kolvenbach |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[2].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[2].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[2].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[2].institutions[0].type | company |
| authorships[2].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Amgen (United States) |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Carl G. KOLVENBACH |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[3].author.id | https://openalex.org/A5079759033 |
| authorships[3].author.orcid | https://orcid.org/0009-0006-0056-2136 |
| authorships[3].author.display_name | T. F. Moran |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[3].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[3].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[3].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[3].institutions[0].type | company |
| authorships[3].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | Amgen (United States) |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Thomas P. MORAN |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[4].author.id | https://openalex.org/A5109155952 |
| authorships[4].author.orcid | |
| authorships[4].author.display_name | Michael McGinley |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[4].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[4].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[4].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[4].institutions[0].type | company |
| authorships[4].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | Amgen (United States) |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Michael McGINLEY |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[5].author.id | https://openalex.org/A5008542033 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-2097-0149 |
| authorships[5].author.display_name | Michael F. Rohde |
| authorships[5].countries | US |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[5].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[5].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[5].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[5].institutions[0].type | company |
| authorships[5].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[5].institutions[0].country_code | US |
| authorships[5].institutions[0].display_name | Amgen (United States) |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Michael F. ROHDE |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[6].author.id | https://openalex.org/A5111039128 |
| authorships[6].author.orcid | |
| authorships[6].author.display_name | Linda O. Narhi |
| authorships[6].countries | US |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I1320553840 |
| authorships[6].affiliations[0].raw_affiliation_string | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| authorships[6].institutions[0].id | https://openalex.org/I1320553840 |
| authorships[6].institutions[0].ror | https://ror.org/03g03ge92 |
| authorships[6].institutions[0].type | company |
| authorships[6].institutions[0].lineage | https://openalex.org/I1320553840 |
| authorships[6].institutions[0].country_code | US |
| authorships[6].institutions[0].display_name | Amgen (United States) |
| authorships[6].author_position | last |
| authorships[6].raw_author_name | Linda O. NARHI |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, U.S.A. |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdf |
| open_access.oa_status | bronze |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Mutual stabilization of detergent resistant subtilisin and heat stable streptomyces subtilisin inhibitor by complex formation. |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11617 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9872999787330627 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1305 |
| primary_topic.subfield.display_name | Biotechnology |
| primary_topic.display_name | Enzyme Production and Characterization |
| related_works | https://openalex.org/W2093489894, https://openalex.org/W2056804237, https://openalex.org/W2018556858, https://openalex.org/W1981377014, https://openalex.org/W1941271094, https://openalex.org/W1985342800, https://openalex.org/W2116258896, https://openalex.org/W1981247471, https://openalex.org/W2020544897, https://openalex.org/W2068723815 |
| cited_by_count | 0 |
| locations_count | 1 |
| best_oa_location.id | doi:10.1271/bbb1961.55.903 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2764346059 |
| best_oa_location.source.issn | 0002-1369, 1881-1280 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | 0002-1369 |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | Agricultural and Biological Chemistry |
| best_oa_location.source.host_organization | https://openalex.org/P4310311648 |
| best_oa_location.source.host_organization_name | Oxford University Press |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310311648 |
| best_oa_location.license | |
| best_oa_location.pdf_url | https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Agricultural and Biological Chemistry |
| best_oa_location.landing_page_url | https://doi.org/10.1271/bbb1961.55.903 |
| primary_location.id | doi:10.1271/bbb1961.55.903 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2764346059 |
| primary_location.source.issn | 0002-1369, 1881-1280 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 0002-1369 |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | Agricultural and Biological Chemistry |
| primary_location.source.host_organization | https://openalex.org/P4310311648 |
| primary_location.source.host_organization_name | Oxford University Press |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310311648 |
| primary_location.license | |
| primary_location.pdf_url | https://www.jstage.jst.go.jp/article/bbb1961/55/4/55_4_903/_pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Agricultural and Biological Chemistry |
| primary_location.landing_page_url | https://doi.org/10.1271/bbb1961.55.903 |
| publication_date | 1991-01-01 |
| publication_year | 1991 |
| referenced_works | https://openalex.org/W6604662711 |
| referenced_works_count | 1 |
| abstract_inverted_index.a | 21, 46, 124 |
| abstract_inverted_index.as | 123, 166 |
| abstract_inverted_index.at | 37, 151 |
| abstract_inverted_index.by | 12, 87, 148, 207, 213 |
| abstract_inverted_index.in | 95 |
| abstract_inverted_index.is | 114, 188 |
| abstract_inverted_index.it | 40, 107, 150 |
| abstract_inverted_index.of | 23, 58, 63, 102, 136, 142, 169, 195, 204, 216, 222, 229 |
| abstract_inverted_index.on | 44 |
| abstract_inverted_index.or | 231 |
| abstract_inverted_index.to | 50, 55, 76, 98, 118, 138, 161, 176, 200, 225 |
| abstract_inverted_index.SDS | 119 |
| abstract_inverted_index.SSI | 26 |
| abstract_inverted_index.Sbt | 24, 77, 103, 137, 197, 206, 230 |
| abstract_inverted_index.The | 0, 140, 158 |
| abstract_inverted_index.and | 5, 25, 30, 78, 104, 120, 156 |
| abstract_inverted_index.gel | 17, 69 |
| abstract_inverted_index.had | 41 |
| abstract_inverted_index.low | 217 |
| abstract_inverted_index.the | 51, 56, 59, 64, 68, 81, 99, 111, 128, 133, 143, 162, 167, 170, 180, 191, 196, 205, 214, 226 |
| abstract_inverted_index.top | 57 |
| abstract_inverted_index.two | 71 |
| abstract_inverted_index.was | 10, 27, 85, 108, 146, 172, 183, 211 |
| abstract_inverted_index.SDS. | 139 |
| abstract_inverted_index.SSI, | 232 |
| abstract_inverted_index.SSI. | 79, 105, 208 |
| abstract_inverted_index.This | 186, 209 |
| abstract_inverted_index.When | 20, 80 |
| abstract_inverted_index.band | 48, 65, 159 |
| abstract_inverted_index.from | 67, 132, 174, 178 |
| abstract_inverted_index.gel. | 60 |
| abstract_inverted_index.none | 221 |
| abstract_inverted_index.only | 54 |
| abstract_inverted_index.room | 38 |
| abstract_inverted_index.same | 82 |
| abstract_inverted_index.than | 190 |
| abstract_inverted_index.that | 110, 234 |
| abstract_inverted_index.then | 31 |
| abstract_inverted_index.upon | 116 |
| abstract_inverted_index.with | 33 |
| abstract_inverted_index.(SSI) | 9 |
| abstract_inverted_index.(Sbt) | 4 |
| abstract_inverted_index.70°C | 175 |
| abstract_inverted_index.Thus, | 106 |
| abstract_inverted_index.about | 184 |
| abstract_inverted_index.alone | 198 |
| abstract_inverted_index.forms | 101 |
| abstract_inverted_index.known | 192 |
| abstract_inverted_index.major | 72 |
| abstract_inverted_index.mixed | 32 |
| abstract_inverted_index.rapid | 154 |
| abstract_inverted_index.sizes | 228 |
| abstract_inverted_index.stems | 131 |
| abstract_inverted_index.this, | 179 |
| abstract_inverted_index.which | 223 |
| abstract_inverted_index.(60°C | 199 |
| abstract_inverted_index.78°C. | 185 |
| abstract_inverted_index.85°C; | 177 |
| abstract_inverted_index.buffer | 36 |
| abstract_inverted_index.during | 126 |
| abstract_inverted_index.eluted | 94 |
| abstract_inverted_index.higher | 189 |
| abstract_inverted_index.intact | 100, 227 |
| abstract_inverted_index.liquid | 91 |
| abstract_inverted_index.sample | 35 |
| abstract_inverted_index.showed | 70 |
| abstract_inverted_index.single | 47 |
| abstract_inverted_index.sodium | 13 |
| abstract_inverted_index.stable | 115 |
| abstract_inverted_index.weight | 219 |
| abstract_inverted_index.(PAGE). | 19 |
| abstract_inverted_index.65°C), | 201 |
| abstract_inverted_index.Sbt/SSI | 83, 112, 144 |
| abstract_inverted_index.between | 2 |
| abstract_inverted_index.complex | 52, 113, 125, 145, 163, 171 |
| abstract_inverted_index.dodecyl | 14 |
| abstract_inverted_index.heating | 149 |
| abstract_inverted_index.mixture | 22, 84 |
| abstract_inverted_index.protein | 93 |
| abstract_inverted_index.reverse | 88 |
| abstract_inverted_index.sulfate | 15 |
| abstract_inverted_index.SDS-PAGE | 34 |
| abstract_inverted_index.Sequence | 61 |
| abstract_inverted_index.abnormal | 42, 129 |
| abstract_inverted_index.analysis | 62 |
| abstract_inverted_index.analyzed | 86 |
| abstract_inverted_index.behavior | 43 |
| abstract_inverted_index.cooling, | 155 |
| abstract_inverted_index.examined | 11, 147 |
| abstract_inverted_index.exposure | 117 |
| abstract_inverted_index.inherent | 134 |
| abstract_inverted_index.migrated | 53 |
| abstract_inverted_index.migrates | 121 |
| abstract_inverted_index.occurred | 236 |
| abstract_inverted_index.species, | 220 |
| abstract_inverted_index.together | 29 |
| abstract_inverted_index.SDS-PAGE. | 157 |
| abstract_inverted_index.SDS-PAGE; | 45, 127 |
| abstract_inverted_index.autolysis | 235 |
| abstract_inverted_index.concluded | 109 |
| abstract_inverted_index.different | 152 |
| abstract_inverted_index.following | 237 |
| abstract_inverted_index.gradually | 164 |
| abstract_inverted_index.increased | 173 |
| abstract_inverted_index.incubated | 28 |
| abstract_inverted_index.inhibitor | 8 |
| abstract_inverted_index.migration | 130 |
| abstract_inverted_index.molecular | 218 |
| abstract_inverted_index.positions | 96 |
| abstract_inverted_index.sequences | 74 |
| abstract_inverted_index.N-terminal | 73 |
| abstract_inverted_index.abnormally | 122 |
| abstract_inverted_index.appearance | 215 |
| abstract_inverted_index.indicating | 202, 233 |
| abstract_inverted_index.phase-high | 89 |
| abstract_inverted_index.resistance | 135 |
| abstract_inverted_index.subtilisin | 3, 7 |
| abstract_inverted_index.transition | 181, 193, 210 |
| abstract_inverted_index.accompanied | 212 |
| abstract_inverted_index.disappeared | 165 |
| abstract_inverted_index.interaction | 1 |
| abstract_inverted_index.performance | 90 |
| abstract_inverted_index.temperature | 168, 182, 187, 194 |
| abstract_inverted_index.Streptomyces | 6 |
| abstract_inverted_index.corresponded | 224 |
| abstract_inverted_index.denaturation | 141 |
| abstract_inverted_index.temperature, | 39 |
| abstract_inverted_index.corresponding | 49, 75, 97, 160 |
| abstract_inverted_index.denaturation. | 238 |
| abstract_inverted_index.stabilization | 203 |
| abstract_inverted_index.temperatures, | 153 |
| abstract_inverted_index.electroblotted | 66 |
| abstract_inverted_index.chromatography, | 92 |
| abstract_inverted_index.electrophoresis | 18 |
| abstract_inverted_index.(SDS)-polyacrylamide | 16 |
| cited_by_percentile_year | |
| countries_distinct_count | 1 |
| institutions_distinct_count | 7 |
| citation_normalized_percentile.value | 0.04906743 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |