Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase Article Swipe

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Tomáš Kouba , T. Kovaĺ , Petra Sudzinová , Jiří Pospíšil , Barbora Brezovská , Jarmila Hnilicová , Hana Šanderová , Martina Janoušková , Michaela Šiková , Petr Halada , Michal Sýkora , Ivan Barvı́k , Jiří Nováček , Mária Trundová , Jarmila Dušková , Tereza Skálová , Uree Chon , Katsuhiko Murakami , Jan Dohnálek , Libor Krásný ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.1101/2020.07.20.211821

SUMMARY RNA synthesis is central to life, and RNA polymerase depends on accessory factors for recovery from stalled states and adaption to environmental changes. Here we investigated the mechanism by which a helicase-like factor HelD recycles RNA polymerase. We report a cryo-EM structure of an unprecedented complex between the Mycobacterium smegmatis RNA polymerase and HelD. The crescent-shaped HelD simultaneously penetrates deep into two RNA polymerase channels that are responsible for DNA binding and substrate delivery to the active site, thereby locking RNA polymerase in an inactive state. We show that HelD prevents non-specific interactions between RNA polymerase and DNA and dissociates transcription elongation complexes, but does not inhibit RNA polymerase binding to the initiation σ factor. The liberated RNA polymerase can either stay dormant, sequestered by HelD, or upon HelD release, restart transcription. Our results provide insights into the architecture and regulation of the highly medically-relevant mycobacterial transcription machinery and define HelD as a clearing factor that removes undesirable nucleic acids from RNA polymerase.

Related Topics

Reverse Transcription Polymerase Chain Reaction

Rna-Dependent Rna Polymerase

Concepts

Polymerase RNA polymerase II RNA polymerase I RNA-dependent RNA polymerase RNA polymerase Transcription factor II D RNA polymerase II holoenzyme RNA Transcription (linguistics) Biology Molecular biology Cell biology DNA Biochemistry Gene expression Promoter Gene Philosophy Linguistics

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Type: preprint
Language: en
Landing Page: https://doi.org/10.1101/2020.07.20.211821
PDF: https://www.biorxiv.org/content/biorxiv/early/2020/07/22/2020.07.20.211821.full.pdf
OA Status: green
Cited By: 2
References: 58
Related Works: 10
OpenAlex ID: https://openalex.org/W3045414302

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DOI: https://doi.org/10.1101/2020.07.20.211821

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Title: Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

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Type: preprint

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Language: en

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Publication year: 2020

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Publication date: 2020-07-22

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Authors: Tomáš Kouba, T. Kovaĺ, Petra Sudzinová, Jiří Pospíšil, Barbora Brezovská, Jarmila Hnilicová, Hana Šanderová, Martina Janoušková, Michaela Šiková, Petr Halada, Michal Sýkora, Ivan Barvı́k, Jiří Nováček, Mária Trundová, Jarmila Dušková, Tereza Skálová, Uree Chon, Katsuhiko Murakami, Jan Dohnálek, Libor Krásný

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Landing page: https://doi.org/10.1101/2020.07.20.211821

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PDF URL: https://www.biorxiv.org/content/biorxiv/early/2020/07/22/2020.07.20.211821.full.pdf

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Concepts: Polymerase, RNA polymerase II, RNA polymerase I, RNA-dependent RNA polymerase, RNA polymerase, Transcription factor II D, RNA polymerase II holoenzyme, RNA, Transcription (linguistics), Biology, Molecular biology, Cell biology, DNA, Biochemistry, Gene expression, Promoter, Gene, Philosophy, Linguistics

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Cited by: 2

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Citations by year (recent): 2023: 1, 2021: 1

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