Natural pigment betacyanin as tracking dye for gel electrophoresis Article Swipe

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Archana Deka , Bhushan Subhash Chougule , Assma Parveen , Jyoti Prasad Lahan , Madhumita Barooah , Robin Chandra Boro ·

YOU? · · 2015 · Open Access · · OA: W2194862252

Several dyes including bromophenol blue are used as tracking dye in gel electrophoresis. The stability of betacyanin extracted from spinach vine fruit ( Basella rubra L.) was studied in relation to degradative factors such as pH ranging from 2 to 12 and at 100oC. Betacyanin pigment was found to be stable at pH range 3-8 while the stability of the pigment at high temperature was found to be moderate. The effectiveness of betacyanin as a tracking dye was checked along with the bromophenol blue for agarose gel electrophoresis. The present study revealed that betacyanin from spinach fruit can be a potential alternative to conventional dye like bromophenol blue used in the loading dye preparation for agarose gel electrophoresis.