New phosphoglucose isomerase mutants of Escherichia coli Article Swipe

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Glucose-6-phosphate isomerase Mutant Biology Escherichia coli Isomerase Mutation Strain (injury) Biochemistry Phenotype Dehydrogenase Molecular biology Genetics Enzyme Gene Anatomy

R T Vinopal , J.D. Hillman , Howard Schulman , William S. Reznikoff , D G Fraenkel ·

YOU? · · 1975 · Open Access · · DOI: https://doi.org/10.1128/jb.122.3.1172-1174.1975 · OA: W1623929313

The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those described earlier. However, they might be preferred for certain physiological studies, and we have prepared for this a new double mutant, strain DF214, with a Mu insertion in pgi and a deletion in zwf (flucose 6-phosphate dehydrogenase).