New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: In Vitro and In Vivo Responses to Environmental Stressors Article Swipe

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Massimiliano Lucidi , Daniela Visaggio , Elisa Prencipe , Francesco Imperi , Giordano Rampioni , Gabriella Cincotti , Livia Leoni , Paolo Visca ·

YOU? · · 2019 · Open Access · · DOI: https://doi.org/10.1128/aem.01334-19

The short-term adaptive response to environmental cues greatly contributes to the ecological success of bacteria, and profound alterations in bacterial gene expression occur in response to physical, chemical, and nutritional stresses. Bacteria belonging to the Acinetobacter genus are ubiquitous inhabitants of soil and water though some species, such as Acinetobacter baumannii , are pathogenic and cause serious concern due to antibiotic resistance. Understanding A. baumannii pathobiology requires adequate genetic tools for gene expression analysis, and to this end we developed user-friendly shuttle vectors to probe the transcriptional responses to different environmental stresses. Vectors were constructed to overcome the problem of antibiotic selection in multidrug-resistant strains and were equipped with suitable reporter systems to facilitate signal detection. By means of these vectors, the transcriptional response of A. baumannii to DNA damage, ethanol exposure, and iron starvation was investigated both in vitro and in vivo , providing insights into A. baumannii adaptation during stress and infection.

Related Topics

Acinetobacter Baumannii

Antimicrobial Resistance

In Vivo

Computational Biology

Pseudomonas Aeruginosa

Neuroscience

Concepts

Acinetobacter baumannii Biology Gene Shuttle vector Multiple drug resistance Gene expression Acinetobacter Bacteria Microbiology Resistome Adaptation (eye) Antibiotic resistance In vivo Genetics Computational biology Vector (molecular biology) Drug resistance Pseudomonas aeruginosa Genome Mobile genetic elements Recombinant DNA Neuroscience

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Type: article
Language: en
Landing Page: https://doi.org/10.1128/aem.01334-19
PDF: https://aem.asm.org/content/aem/85/18/e01334-19.full.pdf
OA Status: bronze
Cited By: 25
References: 75
Related Works: 10
OpenAlex ID: https://openalex.org/W2958135493

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DOI: https://doi.org/10.1128/aem.01334-19

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Title: New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: In Vitro and In Vivo Responses to Environmental Stressors

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Type: article

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Language: en

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Publication year: 2019

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Publication date: 2019-07-16

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Authors: Massimiliano Lucidi, Daniela Visaggio, Elisa Prencipe, Francesco Imperi, Giordano Rampioni, Gabriella Cincotti, Livia Leoni, Paolo Visca

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Landing page: https://doi.org/10.1128/aem.01334-19

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PDF URL: https://aem.asm.org/content/aem/85/18/e01334-19.full.pdf

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Concepts: Acinetobacter baumannii, Biology, Gene, Shuttle vector, Multiple drug resistance, Gene expression, Acinetobacter, Bacteria, Microbiology, Resistome, Adaptation (eye), Antibiotic resistance, In vivo, Genetics, Computational biology, Vector (molecular biology), Drug resistance, Pseudomonas aeruginosa, Genome, Mobile genetic elements, Recombinant DNA, Neuroscience

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Cited by: 25

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Citations by year (recent): 2025: 5, 2024: 5, 2023: 4, 2022: 3, 2021: 6

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References (count): 75

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