Novel exosome biomarker candidates for Alzheimer´s disease unraveled through Mass Spectrometry analysis Article Swipe
Tânia Soares Martins
,
Rui Marçalo
,
C. Beirão Da Cruz E Silva
,
Dário Trindade
,
José Catita
,
Francisco Amado
,
Tânia Melo
,
Ilka Martins Rosa
,
Jonathan Vogelgsang
,
Jens Wiltfang
,
Odete A. B. da Cruz e Silva
,
Ana Gabriela Henriques
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-787466/v2
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-787466/v2
Exosomes are small extracellular vesicles (EVs) present in human biofluids that can transport specific disease-associated molecules. Consequently blood-derived exosomes have emerged as important peripheral biomarker sources for a wide range of diseases, among them Alzheimer’s disease (AD). Although there is no effective cure for AD, an accurate diagnosis, relying on easily accessible peripheral biofluids, is still necessary to discriminate this disease from other dementias, test potential therapies and even monitor rate of disease progression. The ultimate goal is to produce a cost-effective and widely available alternative, which can also be employed as a first clinical screen. In this study, EVs with exosome-like characteristics were isolated from serum of Controls and AD cases through precipitation- and column-based methods, followed by mass spectrometry analysis. The resulting proteomes were characterized by Gene Ontology (GO) functional enrichment and multivariate analyses. Although GO terms were similar for exosomes proteomes of Controls and ADs, using both methodologies, a clear segregation of disease cases was obtained when using the precipitation-based method. Nine significantly different abundant proteins were identified between Controls and AD cases, representing putative biomarker candidate targets. Among them AACT and C4BPα, two Aβ-binding proteins, whose exosome levels were further validated in individuals from independent cohorts using antibody-based approaches. The findings discussed represent an important contribution to the identification of novel exosomal biomarker candidates useful as potential blood-based tools for AD diagnosis.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-787466/v2
- OA Status
- gold
- References
- 63
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3194250305Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-787466/v2Digital Object Identifier
- Title
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Novel exosome biomarker candidates for Alzheimer´s disease unraveled through Mass Spectrometry analysisWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-10-01Full publication date if available
- Authors
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Tânia Soares Martins, Rui Marçalo, C. Beirão Da Cruz E Silva, Dário Trindade, José Catita, Francisco Amado, Tânia Melo, Ilka Martins Rosa, Jonathan Vogelgsang, Jens Wiltfang, Odete A. B. da Cruz e Silva, Ana Gabriela HenriquesList of authors in order
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https://doi.org/10.21203/rs.3.rs-787466/v2Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.21203/rs.3.rs-787466/v2Direct OA link when available
- Concepts
-
Biomarker, Exosome, Mass spectrometry, Disease, Computational biology, Microvesicles, Chemistry, Medicine, Internal medicine, Chromatography, Biology, Biochemistry, Gene, microRNATop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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63Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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