Observing hidden neuronal states in experiments Article Swipe
Dmitry V. Amakhin
,
Anton V. Chizhov
,
Mathieu Desroches
,
Jan Sieber
,
Serafim Rodrigues
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pcbi.1013748
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pcbi.1013748
In this article we demonstrate a general protocol for constructing systematically experimental steady-state bifurcation diagrams for electrophysiologically active cells. We perform our experiments on entorhinal cortex neurons, both excitatory (pyramidal neurons) and inhibitiory (interneurons). A slowly ramped voltage-clamp electrophysiology protocol serves as closed-loop feedback controlled experiment for the subsequent current-clamp open-loop protocol on the same cell. In this way, the voltage-clamped experiment determines dynamically stable and unstable (hidden) steady states of the current-clamp experiment. The transitions between observable steady states and observable spiking states in the current-clamp experiment provide partial evidence for stability and bifurcations of the steady states. This technique for completing steady-state bifurcation diagrams in a model-independent way expands support for model validation to otherwise inaccessible regions of the phase space. Overlaying the voltage-clamp and current-clamp protocols leads to an experimental validation of the classical slow-fast dissection method introduced by J. Rinzel in the 1980s and routinely applied ever since in order to analyse slow-fast neuronal models. Our approach opens doors to observing further complex hidden states with more advanced control strategies, allowing to control real cells beyond pharmacological manipulations.
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- Type
- article
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- en
- Landing Page
- https://doi.org/10.1371/journal.pcbi.1013748
- OA Status
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- References
- 35
- OpenAlex ID
- https://openalex.org/W4417131784
All OpenAlex metadata
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https://doi.org/10.1371/journal.pcbi.1013748Digital Object Identifier
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Observing hidden neuronal states in experimentsWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-12-08Full publication date if available
- Authors
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Dmitry V. Amakhin, Anton V. Chizhov, Mathieu Desroches, Jan Sieber, Serafim RodriguesList of authors in order
- Landing page
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https://doi.org/10.1371/journal.pcbi.1013748Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1371/journal.pcbi.1013748Direct OA link when available
- Cited by
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0Total citation count in OpenAlex
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35Number of works referenced by this work
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