One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particles Article Swipe
Pavel Mikel
,
Petra Vašíčková
,
Petr Králík
·
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-017-17951-5
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-017-17951-5
MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it against the effects of ubiquitous nucleases. Such particles are able to mimic ssRNA viruses and, thus, may serve as the process control for molecular detection and quantification of such agents in several kinds of matrices, vaccines and vaccine candidates, drug delivery systems, and systems for the display of immunologically active peptides or nanomachines. Currently, there are several different in vivo plasmid-driven packaging systems for production of MS2 PLP. In order to combine all the advantages of the available systems and to upgrade and simplify the production and purification of MS2 PLP, a one-plasmid double-expression His-tag system was designed. The described system utilizes a unique fusion insertional mutation enabling purification of particles using His-tag affinity. Using this new production system, highly pure MS2 PLP can be quickly produced and purified by a fast performance liquid chromatography (FPLC) approach. The system can be easily adapted to produce other MS2 PLP with different properties.
Related Topics
Concepts
Bacteriophage MS2
Capsid
Plasmid
Fast protein liquid chromatography
Computational biology
Bacteriophage
Chemistry
Virus-like particle
DNA
Biology
Recombinant DNA
Escherichia coli
Biochemistry
Gene
Enzyme
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41598-017-17951-5
- https://www.nature.com/articles/s41598-017-17951-5.pdf
- OA Status
- gold
- Cited By
- 19
- References
- 34
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2774257091
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2774257091Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1038/s41598-017-17951-5Digital Object Identifier
- Title
-
One-plasmid double-expression His-tag system for rapid production and easy purification of MS2 phage-like particlesWork title
- Type
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articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2017Year of publication
- Publication date
-
2017-12-07Full publication date if available
- Authors
-
Pavel Mikel, Petra Vašíčková, Petr KrálíkList of authors in order
- Landing page
-
https://doi.org/10.1038/s41598-017-17951-5Publisher landing page
- PDF URL
-
https://www.nature.com/articles/s41598-017-17951-5.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
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https://www.nature.com/articles/s41598-017-17951-5.pdfDirect OA link when available
- Concepts
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Bacteriophage MS2, Capsid, Plasmid, Fast protein liquid chromatography, Computational biology, Bacteriophage, Chemistry, Virus-like particle, DNA, Biology, Recombinant DNA, Escherichia coli, Biochemistry, Gene, EnzymeTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
19Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 5, 2024: 2, 2023: 4, 2022: 4, 2021: 1Per-year citation counts (last 5 years)
- References (count)
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34Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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