On‐site genetic analysis for species identification using lab‐on‐a‐chip Article Swipe
Ryan Wimbles
,
Louise M. Melling
,
Bradley Cain
,
Naomi Davies Walsh
,
Jason M. Doherty
,
Bridget Johnson
,
Kirsty J. Shaw
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1002/ece3.7053
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1002/ece3.7053
This paper presents a microfluidic device capable of performing genetic analysis on dung samples to identify White Rhinoceros ( Ceratotherium simum ). The development of a microfluidic device, which can be used in the field, offers a portable and cost‐effective solution for DNA analysis and species identification to aid conservation efforts. Optimization of the DNA extraction processes produced equivalent yields compared to conventional kit‐based methods within just 5 minutes. The use of a color‐changing loop‐mediated isothermal amplification reaction for simultaneous detection of the cytochrome B sequence of C. simum enabled positive results to be obtained within as little as 30 minutes. Field testing was performed at Knowsley Safari to demonstrate real‐world applicability of the microfluidic device for testing of biological samples.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/ece3.7053
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7053
- OA Status
- gold
- Cited By
- 20
- References
- 30
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3128514449
All OpenAlex metadata
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- OpenAlex ID
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https://openalex.org/W3128514449Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/ece3.7053Digital Object Identifier
- Title
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On‐site genetic analysis for species identification using lab‐on‐a‐chipWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-01-29Full publication date if available
- Authors
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Ryan Wimbles, Louise M. Melling, Bradley Cain, Naomi Davies Walsh, Jason M. Doherty, Bridget Johnson, Kirsty J. ShawList of authors in order
- Landing page
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https://doi.org/10.1002/ece3.7053Publisher landing page
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7053Direct link to full text PDF
- Open access
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.7053Direct OA link when available
- Concepts
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Rhinoceros, Microfluidics, Loop-mediated isothermal amplification, Identification (biology), Lab-on-a-chip, Computer science, DNA extraction, Biochemical engineering, Biological system, Biology, Nanotechnology, DNA, Ecology, Engineering, Polymerase chain reaction, Materials science, Genetics, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
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20Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2024: 4, 2023: 7, 2022: 4, 2021: 2Per-year citation counts (last 5 years)
- References (count)
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30Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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