Optical Tweezers: A Comprehensive Tutorial from Calibration to\n Applications Article Swipe

PDF

Jan Gieseler , Juan Ruben Gomez-Solano , Alessandro Magazzù , Isaac Pérez Castillo , Laura Pérez García , Marta Gironella-Torrent , Xavier Viader-Godoy , Félix Ritort , Giuseppe Pesce , Alejandro V. Arzola , Karen Volke-Sepúlveda , Giovanni Volpe ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.48550/arxiv.2004.05246

Since their invention in 1986 by Arthur Ashkin and colleagues, optical\ntweezers have become an essential tool in several fields of physics,\nspectroscopy, biology, nanotechnology, and thermodynamics. In this Tutorial, we\nprovide a primer on how to calibrate optical tweezers and how to use them for\nadvanced applications. After a brief general introduction on optical tweezers,\nwe focus on describing and comparing the various available calibration\ntechniques. Then, we discuss some cutting-edge applications of optical tweezers\nin a liquid medium, namely to study single-molecule and single-cell mechanics,\nmicrorheology, colloidal interactions, statistical physics, and transport\nphenomena. Finally, we consider optical tweezers in vacuum, where the absence\nof a viscous medium offers vastly different dynamics and presents new\nchallenges. We conclude with some perspectives for the field and the future\napplication of optical tweezers. This Tutorial provides both a step-by-step\nguide ideal for non-specialists entering the field and a comprehensive manual\nof advanced techniques useful for expert practitioners. All the examples are\ncomplemented by the sample data and software necessary to reproduce them.\n

Related Topics

Concepts

Optical tweezers Microrheology Nanotechnology Calibration Field (mathematics) Computer science Physics Optics Materials science Mathematics Rheology Thermodynamics Quantum mechanics Pure mathematics

Metadata

Type: preprint
Language: en
Landing Page: http://arxiv.org/abs/2004.05246
PDF: https://arxiv.org/pdf/2004.05246
OA Status: green
Cited By: 8
Related Works: 10
OpenAlex ID: https://openalex.org/W4287815913

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4287815913

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.48550/arxiv.2004.05246

Digital Object Identifier
Title: Optical Tweezers: A Comprehensive Tutorial from Calibration to\n Applications

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2020

Year of publication
Publication date: 2020-04-10

Full publication date if available
Authors: Jan Gieseler, Juan Ruben Gomez-Solano, Alessandro Magazzù, Isaac Pérez Castillo, Laura Pérez García, Marta Gironella-Torrent, Xavier Viader-Godoy, Félix Ritort, Giuseppe Pesce, Alejandro V. Arzola, Karen Volke-Sepúlveda, Giovanni Volpe

List of authors in order
Landing page: https://arxiv.org/abs/2004.05246

Publisher landing page
PDF URL: https://arxiv.org/pdf/2004.05246

Direct link to full text PDF
Open access: Yes

Whether a free full text is available
OA status: green

Open access status per OpenAlex
OA URL: https://arxiv.org/pdf/2004.05246

Direct OA link when available
Concepts: Optical tweezers, Microrheology, Nanotechnology, Calibration, Field (mathematics), Computer science, Physics, Optics, Materials science, Mathematics, Rheology, Thermodynamics, Quantum mechanics, Pure mathematics

Top concepts (fields/topics) attached by OpenAlex
Cited by: 8

Total citation count in OpenAlex
Citations by year (recent): 2022: 3, 2021: 3, 2020: 2

Per-year citation counts (last 5 years)
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4287815913
doi	https://doi.org/10.48550/arxiv.2004.05246
ids.openalex	https://openalex.org/W4287815913
fwci	1.07838105
type	preprint
title	Optical Tweezers: A Comprehensive Tutorial from Calibration to\n Applications
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T10490
topics[0].field.id	https://openalex.org/fields/31
topics[0].field.display_name	Physics and Astronomy
topics[0].score	0.9901999831199646
topics[0].domain.id	https://openalex.org/domains/3
topics[0].domain.display_name	Physical Sciences
topics[0].subfield.id	https://openalex.org/subfields/3107
topics[0].subfield.display_name	Atomic and Molecular Physics, and Optics
topics[0].display_name	Orbital Angular Momentum in Optics
topics[1].id	https://openalex.org/T11255
topics[1].field.id	https://openalex.org/fields/22
topics[1].field.display_name	Engineering
topics[1].score	0.9376000165939331
topics[1].domain.id	https://openalex.org/domains/3
topics[1].domain.display_name	Physical Sciences
topics[1].subfield.id	https://openalex.org/subfields/2204
topics[1].subfield.display_name	Biomedical Engineering
topics[1].display_name	Microfluidic and Bio-sensing Technologies
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C20198109
concepts[0].level	2
concepts[0].score	0.9382768869400024
concepts[0].wikidata	https://www.wikidata.org/wiki/Q1066633
concepts[0].display_name	Optical tweezers
concepts[1].id	https://openalex.org/C34123561
concepts[1].level	3
concepts[1].score	0.6831806898117065
concepts[1].wikidata	https://www.wikidata.org/wiki/Q6840000
concepts[1].display_name	Microrheology
concepts[2].id	https://openalex.org/C171250308
concepts[2].level	1
concepts[2].score	0.45362889766693115
concepts[2].wikidata	https://www.wikidata.org/wiki/Q11468
concepts[2].display_name	Nanotechnology
concepts[3].id	https://openalex.org/C165838908
concepts[3].level	2
concepts[3].score	0.4222773313522339
concepts[3].wikidata	https://www.wikidata.org/wiki/Q736777
concepts[3].display_name	Calibration
concepts[4].id	https://openalex.org/C9652623
concepts[4].level	2
concepts[4].score	0.412450909614563
concepts[4].wikidata	https://www.wikidata.org/wiki/Q190109
concepts[4].display_name	Field (mathematics)
concepts[5].id	https://openalex.org/C41008148
concepts[5].level	0
concepts[5].score	0.41132909059524536
concepts[5].wikidata	https://www.wikidata.org/wiki/Q21198
concepts[5].display_name	Computer science
concepts[6].id	https://openalex.org/C121332964
concepts[6].level	0
concepts[6].score	0.331134557723999
concepts[6].wikidata	https://www.wikidata.org/wiki/Q413
concepts[6].display_name	Physics
concepts[7].id	https://openalex.org/C120665830
concepts[7].level	1
concepts[7].score	0.21418681740760803
concepts[7].wikidata	https://www.wikidata.org/wiki/Q14620
concepts[7].display_name	Optics
concepts[8].id	https://openalex.org/C192562407
concepts[8].level	0
concepts[8].score	0.16330763697624207
concepts[8].wikidata	https://www.wikidata.org/wiki/Q228736
concepts[8].display_name	Materials science
concepts[9].id	https://openalex.org/C33923547
concepts[9].level	0
concepts[9].score	0.08944836258888245
concepts[9].wikidata	https://www.wikidata.org/wiki/Q395
concepts[9].display_name	Mathematics
concepts[10].id	https://openalex.org/C200990466
concepts[10].level	2
concepts[10].score	0.07577240467071533
concepts[10].wikidata	https://www.wikidata.org/wiki/Q271707
concepts[10].display_name	Rheology
concepts[11].id	https://openalex.org/C97355855
concepts[11].level	1
concepts[11].score	0.0
concepts[11].wikidata	https://www.wikidata.org/wiki/Q11473
concepts[11].display_name	Thermodynamics
concepts[12].id	https://openalex.org/C62520636
concepts[12].level	1
concepts[12].score	0.0
concepts[12].wikidata	https://www.wikidata.org/wiki/Q944
concepts[12].display_name	Quantum mechanics
concepts[13].id	https://openalex.org/C202444582
concepts[13].level	1
concepts[13].score	0.0
concepts[13].wikidata	https://www.wikidata.org/wiki/Q837863
concepts[13].display_name	Pure mathematics
keywords[0].id	https://openalex.org/keywords/optical-tweezers
keywords[0].score	0.9382768869400024
keywords[0].display_name	Optical tweezers
keywords[1].id	https://openalex.org/keywords/microrheology
keywords[1].score	0.6831806898117065
keywords[1].display_name	Microrheology
keywords[2].id	https://openalex.org/keywords/nanotechnology
keywords[2].score	0.45362889766693115
keywords[2].display_name	Nanotechnology
keywords[3].id	https://openalex.org/keywords/calibration
keywords[3].score	0.4222773313522339
keywords[3].display_name	Calibration
keywords[4].id	https://openalex.org/keywords/field
keywords[4].score	0.412450909614563
keywords[4].display_name	Field (mathematics)
keywords[5].id	https://openalex.org/keywords/computer-science
keywords[5].score	0.41132909059524536
keywords[5].display_name	Computer science
keywords[6].id	https://openalex.org/keywords/physics
keywords[6].score	0.331134557723999
keywords[6].display_name	Physics
keywords[7].id	https://openalex.org/keywords/optics
keywords[7].score	0.21418681740760803
keywords[7].display_name	Optics
keywords[8].id	https://openalex.org/keywords/materials-science
keywords[8].score	0.16330763697624207
keywords[8].display_name	Materials science
keywords[9].id	https://openalex.org/keywords/mathematics
keywords[9].score	0.08944836258888245
keywords[9].display_name	Mathematics
keywords[10].id	https://openalex.org/keywords/rheology
keywords[10].score	0.07577240467071533
keywords[10].display_name	Rheology
language	en
locations[0].id	pmh:oai:arXiv.org:2004.05246
locations[0].is_oa	True
locations[0].source.id	https://openalex.org/S4306400194
locations[0].source.issn
locations[0].source.type	repository
locations[0].source.is_oa	True
locations[0].source.issn_l
locations[0].source.is_core	False
locations[0].source.is_in_doaj	False
locations[0].source.display_name	arXiv (Cornell University)
locations[0].source.host_organization	https://openalex.org/I205783295
locations[0].source.host_organization_name	Cornell University
locations[0].source.host_organization_lineage	https://openalex.org/I205783295
locations[0].license
locations[0].pdf_url	https://arxiv.org/pdf/2004.05246
locations[0].version	submittedVersion
locations[0].raw_type	text
locations[0].license_id
locations[0].is_accepted	False
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	http://arxiv.org/abs/2004.05246
indexed_in	arxiv
authorships[0].author.id	https://openalex.org/A5026025280
authorships[0].author.orcid	https://orcid.org/0000-0003-1011-5269
authorships[0].author.display_name	Jan Gieseler
authorships[0].author_position	first
authorships[0].raw_author_name	Gieseler, Jan
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5085677498
authorships[1].author.orcid	https://orcid.org/0000-0003-2475-1151
authorships[1].author.display_name	Juan Ruben Gomez-Solano
authorships[1].author_position	middle
authorships[1].raw_author_name	Gomez-Solano, Juan Ruben
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5036159211
authorships[2].author.orcid	https://orcid.org/0000-0003-1247-2702
authorships[2].author.display_name	Alessandro Magazzù
authorships[2].author_position	middle
authorships[2].raw_author_name	Magazzù, Alessandro
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5007031065
authorships[3].author.orcid	https://orcid.org/0000-0001-7622-9440
authorships[3].author.display_name	Isaac Pérez Castillo
authorships[3].author_position	middle
authorships[3].raw_author_name	Castillo, Isaac Pérez
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5103816048
authorships[4].author.orcid
authorships[4].author.display_name	Laura Pérez García
authorships[4].author_position	middle
authorships[4].raw_author_name	García, Laura Pérez
authorships[4].is_corresponding	False
authorships[5].author.id	https://openalex.org/A5071644714
authorships[5].author.orcid	https://orcid.org/0000-0003-2210-8779
authorships[5].author.display_name	Marta Gironella-Torrent
authorships[5].author_position	middle
authorships[5].raw_author_name	Gironella-Torrent, Marta
authorships[5].is_corresponding	False
authorships[6].author.id	https://openalex.org/A5014563192
authorships[6].author.orcid	https://orcid.org/0000-0002-9324-8915
authorships[6].author.display_name	Xavier Viader-Godoy
authorships[6].author_position	middle
authorships[6].raw_author_name	Viader-Godoy, Xavier
authorships[6].is_corresponding	False
authorships[7].author.id	https://openalex.org/A5086595191
authorships[7].author.orcid	https://orcid.org/0000-0002-4869-5003
authorships[7].author.display_name	Félix Ritort
authorships[7].author_position	middle
authorships[7].raw_author_name	Ritort, Felix
authorships[7].is_corresponding	False
authorships[8].author.id	https://openalex.org/A5064306329
authorships[8].author.orcid	https://orcid.org/0000-0001-5616-8599
authorships[8].author.display_name	Giuseppe Pesce
authorships[8].author_position	middle
authorships[8].raw_author_name	Pesce, Giuseppe
authorships[8].is_corresponding	False
authorships[9].author.id	https://openalex.org/A5035204252
authorships[9].author.orcid	https://orcid.org/0000-0002-4860-6330
authorships[9].author.display_name	Alejandro V. Arzola
authorships[9].author_position	middle
authorships[9].raw_author_name	Arzola, Alejandro V.
authorships[9].is_corresponding	False
authorships[10].author.id	https://openalex.org/A5003736376
authorships[10].author.orcid	https://orcid.org/0000-0001-6048-4244
authorships[10].author.display_name	Karen Volke-Sepúlveda
authorships[10].author_position	middle
authorships[10].raw_author_name	Volke-Sepulveda, Karen
authorships[10].is_corresponding	False
authorships[11].author.id	https://openalex.org/A5010589630
authorships[11].author.orcid	https://orcid.org/0000-0001-5057-1846
authorships[11].author.display_name	Giovanni Volpe
authorships[11].author_position	last
authorships[11].raw_author_name	Volpe, Giovanni
authorships[11].is_corresponding	False
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://arxiv.org/pdf/2004.05246
open_access.oa_status	green
open_access.any_repository_has_fulltext	False
created_date	2022-07-26T00:00:00
display_name	Optical Tweezers: A Comprehensive Tutorial from Calibration to\n Applications
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T10490
primary_topic.field.id	https://openalex.org/fields/31
primary_topic.field.display_name	Physics and Astronomy
primary_topic.score	0.9901999831199646
primary_topic.domain.id	https://openalex.org/domains/3
primary_topic.domain.display_name	Physical Sciences
primary_topic.subfield.id	https://openalex.org/subfields/3107
primary_topic.subfield.display_name	Atomic and Molecular Physics, and Optics
primary_topic.display_name	Orbital Angular Momentum in Optics
related_works	https://openalex.org/W2511115738, https://openalex.org/W2016281676, https://openalex.org/W1975379701, https://openalex.org/W2151646901, https://openalex.org/W1966774522, https://openalex.org/W2270084434, https://openalex.org/W2600396459, https://openalex.org/W2793084139, https://openalex.org/W4387379713, https://openalex.org/W170056996
cited_by_count	8
counts_by_year[0].year	2022
counts_by_year[0].cited_by_count	3
counts_by_year[1].year	2021
counts_by_year[1].cited_by_count	3
counts_by_year[2].year	2020
counts_by_year[2].cited_by_count	2
locations_count	1
best_oa_location.id	pmh:oai:arXiv.org:2004.05246
best_oa_location.is_oa	True
best_oa_location.source.id	https://openalex.org/S4306400194
best_oa_location.source.issn
best_oa_location.source.type	repository
best_oa_location.source.is_oa	True
best_oa_location.source.issn_l
best_oa_location.source.is_core	False
best_oa_location.source.is_in_doaj	False
best_oa_location.source.display_name	arXiv (Cornell University)
best_oa_location.source.host_organization	https://openalex.org/I205783295
best_oa_location.source.host_organization_name	Cornell University
best_oa_location.source.host_organization_lineage	https://openalex.org/I205783295
best_oa_location.license
best_oa_location.pdf_url	https://arxiv.org/pdf/2004.05246
best_oa_location.version	submittedVersion
best_oa_location.raw_type	text
best_oa_location.license_id
best_oa_location.is_accepted	False
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	http://arxiv.org/abs/2004.05246
primary_location.id	pmh:oai:arXiv.org:2004.05246
primary_location.is_oa	True
primary_location.source.id	https://openalex.org/S4306400194
primary_location.source.issn
primary_location.source.type	repository
primary_location.source.is_oa	True
primary_location.source.issn_l
primary_location.source.is_core	False
primary_location.source.is_in_doaj	False
primary_location.source.display_name	arXiv (Cornell University)
primary_location.source.host_organization	https://openalex.org/I205783295
primary_location.source.host_organization_name	Cornell University
primary_location.source.host_organization_lineage	https://openalex.org/I205783295
primary_location.license
primary_location.pdf_url	https://arxiv.org/pdf/2004.05246
primary_location.version	submittedVersion
primary_location.raw_type	text
primary_location.license_id
primary_location.is_accepted	False
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	http://arxiv.org/abs/2004.05246
publication_date	2020-04-10
publication_year	2020
referenced_works_count	0
abstract_inverted_index.a	29, 45, 70, 96, 124, 133
abstract_inverted_index.In	25
abstract_inverted_index.We	106
abstract_inverted_index.an	13
abstract_inverted_index.by	5, 146
abstract_inverted_index.in	3, 16, 91
abstract_inverted_index.of	19, 67, 117
abstract_inverted_index.on	31, 49, 53
abstract_inverted_index.to	33, 39, 74, 153
abstract_inverted_index.we	62, 87
abstract_inverted_index.All	142
abstract_inverted_index.and	8, 23, 37, 55, 77, 84, 103, 114, 132, 150
abstract_inverted_index.for	111, 127, 139
abstract_inverted_index.how	32, 38
abstract_inverted_index.the	57, 94, 112, 115, 130, 143, 147
abstract_inverted_index.use	40
abstract_inverted_index.1986	4
abstract_inverted_index.This	120
abstract_inverted_index.both	123
abstract_inverted_index.data	149
abstract_inverted_index.have	11
abstract_inverted_index.some	64, 109
abstract_inverted_index.them	41
abstract_inverted_index.this	26
abstract_inverted_index.tool	15
abstract_inverted_index.with	108
abstract_inverted_index.After	44
abstract_inverted_index.Since	0
abstract_inverted_index.Then,	61
abstract_inverted_index.brief	46
abstract_inverted_index.field	113, 131
abstract_inverted_index.focus	52
abstract_inverted_index.ideal	126
abstract_inverted_index.study	75
abstract_inverted_index.their	1
abstract_inverted_index.where	93
abstract_inverted_index.Arthur	6
abstract_inverted_index.Ashkin	7
abstract_inverted_index.become	12
abstract_inverted_index.expert	140
abstract_inverted_index.fields	18
abstract_inverted_index.liquid	71
abstract_inverted_index.medium	98
abstract_inverted_index.namely	73
abstract_inverted_index.offers	99
abstract_inverted_index.primer	30
abstract_inverted_index.sample	148
abstract_inverted_index.useful	138
abstract_inverted_index.vastly	100
abstract_inverted_index.discuss	63
abstract_inverted_index.general	47
abstract_inverted_index.medium,	72
abstract_inverted_index.optical	35, 50, 68, 89, 118
abstract_inverted_index.several	17
abstract_inverted_index.them.\n	155
abstract_inverted_index.vacuum,	92
abstract_inverted_index.various	58
abstract_inverted_index.viscous	97
abstract_inverted_index.Finally,	86
abstract_inverted_index.Tutorial	121
abstract_inverted_index.advanced	136
abstract_inverted_index.biology,	21
abstract_inverted_index.conclude	107
abstract_inverted_index.consider	88
abstract_inverted_index.dynamics	102
abstract_inverted_index.entering	129
abstract_inverted_index.examples	144
abstract_inverted_index.physics,	83
abstract_inverted_index.presents	104
abstract_inverted_index.provides	122
abstract_inverted_index.software	151
abstract_inverted_index.tweezers	36, 90
abstract_inverted_index.Tutorial,	27
abstract_inverted_index.available	59
abstract_inverted_index.calibrate	34
abstract_inverted_index.colloidal	80
abstract_inverted_index.comparing	56
abstract_inverted_index.different	101
abstract_inverted_index.essential	14
abstract_inverted_index.invention	2
abstract_inverted_index.necessary	152
abstract_inverted_index.reproduce	154
abstract_inverted_index.tweezers.	119
abstract_inverted_index.describing	54
abstract_inverted_index.manual\nof	135
abstract_inverted_index.techniques	137
abstract_inverted_index.absence\nof	95
abstract_inverted_index.colleagues,	9
abstract_inverted_index.single-cell	78
abstract_inverted_index.statistical	82
abstract_inverted_index.we\nprovide	28
abstract_inverted_index.applications	66
abstract_inverted_index.cutting-edge	65
abstract_inverted_index.introduction	48
abstract_inverted_index.perspectives	110
abstract_inverted_index.tweezers\nin	69
abstract_inverted_index.applications.	43
abstract_inverted_index.comprehensive	134
abstract_inverted_index.for\nadvanced	42
abstract_inverted_index.interactions,	81
abstract_inverted_index.tweezers,\nwe	51
abstract_inverted_index.practitioners.	141
abstract_inverted_index.nanotechnology,	22
abstract_inverted_index.non-specialists	128
abstract_inverted_index.single-molecule	76
abstract_inverted_index.thermodynamics.	24
abstract_inverted_index.new\nchallenges.	105
abstract_inverted_index.are\ncomplemented	145
abstract_inverted_index.optical\ntweezers	10
abstract_inverted_index.future\napplication	116
abstract_inverted_index.step-by-step\nguide	125
abstract_inverted_index.transport\nphenomena.	85
abstract_inverted_index.physics,\nspectroscopy,	20
abstract_inverted_index.calibration\ntechniques.	60
abstract_inverted_index.mechanics,\nmicrorheology,	79
cited_by_percentile_year.max	97
cited_by_percentile_year.min	94
countries_distinct_count	0
institutions_distinct_count	12
citation_normalized_percentile.value	0.80839911
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False