Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format Article Swipe

View

Jacob Fry , Jean Lee , Julie McAuley , Jessica L. Porter , Ian R. Monk , Samuel T. Martin , David J. Collins , Gregory J. Barbante , N.J. FitzGerald , Timothy P. Stinear ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.1021/acsomega.4c05784

The Coronavirus disease 2019 (COVID-19) pandemic has supercharged innovation in the field of molecular diagnostics and led to the exploration of systems that permit the autonomous identification of airborne infectious agents. Airborne virus detection is an emerging approach for determining exposure risk, although current methods limit intervention timeliness. Here, we explore reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for one-pot detection of Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) (SCV2) run on membrane filters suitable for micro-air-filtration of airborne viruses. We use a design of experiments statistical framework to establish the optimal additive composition for running RT-LAMP on membrane filters. Using SCV2 liquid spike-in experiments and fluorescence detection, we show that single-pot RT-LAMP on glass fiber filters reliably detected 0.10 50% tissue culture infectious dose (TCID₅₀) SCV2 per reaction (3600 E-gene copies) and is an order of magnitude more sensitive than conventional RT-LAMP.

Related Topics

Reverse Transcriptase

Chemistry

Biology

Polymerase Chain Reaction

Medicine

Infectious Diseases (Medical Specialty)

Concepts

Loop-mediated isothermal amplification Reverse Transcription Loop-mediated Isothermal Amplification In situ Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Filtration (mathematics) Coronavirus disease 2019 (COVID-19) Materials science Loop (graph theory) 2019-20 coronavirus outbreak Virology Reverse transcriptase Chemistry Biology Polymerase chain reaction Medicine Genetics Gene Infectious disease (medical specialty) Outbreak Combinatorics Pathology Organic chemistry DNA Statistics Mathematics Disease

Metadata

Type: article
Language: en
Landing Page: https://doi.org/10.1021/acsomega.4c05784
OA Status: gold
Cited By: 2
References: 44
Related Works: 10
OpenAlex ID: https://openalex.org/W4402660400

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4402660400

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.1021/acsomega.4c05784

Digital Object Identifier
Title: Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format

Work title
Type: article

OpenAlex work type
Language: en

Primary language
Publication year: 2024

Year of publication
Publication date: 2024-09-20

Full publication date if available
Authors: Jacob Fry, Jean Lee, Julie McAuley, Jessica L. Porter, Ian R. Monk, Samuel T. Martin, David J. Collins, Gregory J. Barbante, N.J. FitzGerald, Timothy P. Stinear

List of authors in order
Landing page: https://doi.org/10.1021/acsomega.4c05784

Publisher landing page
Open access: Yes

Whether a free full text is available
OA status: gold

Open access status per OpenAlex
OA URL: https://doi.org/10.1021/acsomega.4c05784

Direct OA link when available
Concepts: Loop-mediated isothermal amplification, Reverse Transcription Loop-mediated Isothermal Amplification, In situ, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Filtration (mathematics), Coronavirus disease 2019 (COVID-19), Materials science, Loop (graph theory), 2019-20 coronavirus outbreak, Virology, Reverse transcriptase, Chemistry, Biology, Polymerase chain reaction, Medicine, Genetics, Gene, Infectious disease (medical specialty), Outbreak, Combinatorics, Pathology, Organic chemistry, DNA, Statistics, Mathematics, Disease

Top concepts (fields/topics) attached by OpenAlex
Cited by: 2

Total citation count in OpenAlex
Citations by year (recent): 2025: 2

Per-year citation counts (last 5 years)
References (count): 44

Number of works referenced by this work
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4402660400
doi	https://doi.org/10.1021/acsomega.4c05784
ids.doi	https://doi.org/10.1021/acsomega.4c05784
ids.pmid	https://pubmed.ncbi.nlm.nih.gov/39372017
ids.openalex	https://openalex.org/W4402660400
fwci	0.73505193
type	article
title	Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format
biblio.issue	39
biblio.volume	9
biblio.last_page	40840
biblio.first_page	40832
topics[0].id	https://openalex.org/T11393
topics[0].field.id	https://openalex.org/fields/22
topics[0].field.display_name	Engineering
topics[0].score	0.9998999834060669
topics[0].domain.id	https://openalex.org/domains/3
topics[0].domain.display_name	Physical Sciences
topics[0].subfield.id	https://openalex.org/subfields/2204
topics[0].subfield.display_name	Biomedical Engineering
topics[0].display_name	Biosensors and Analytical Detection
topics[1].id	https://openalex.org/T11754
topics[1].field.id	https://openalex.org/fields/27
topics[1].field.display_name	Medicine
topics[1].score	0.9991999864578247
topics[1].domain.id	https://openalex.org/domains/4
topics[1].domain.display_name	Health Sciences
topics[1].subfield.id	https://openalex.org/subfields/2725
topics[1].subfield.display_name	Infectious Diseases
topics[1].display_name	SARS-CoV-2 detection and testing
topics[2].id	https://openalex.org/T10207
topics[2].field.id	https://openalex.org/fields/13
topics[2].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[2].score	0.9889000058174133
topics[2].domain.id	https://openalex.org/domains/1
topics[2].domain.display_name	Life Sciences
topics[2].subfield.id	https://openalex.org/subfields/1312
topics[2].subfield.display_name	Molecular Biology
topics[2].display_name	Advanced biosensing and bioanalysis techniques
funders[0].id	https://openalex.org/F4320335334
funders[0].ror
funders[0].display_name	Defence Science and Technology Group
is_xpac	False
apc_list.value	1685
apc_list.currency	USD
apc_list.value_usd	1685
apc_paid.value	1685
apc_paid.currency	USD
apc_paid.value_usd	1685
concepts[0].id	https://openalex.org/C60635243
concepts[0].level	3
concepts[0].score	0.8345302939414978
concepts[0].wikidata	https://www.wikidata.org/wiki/Q6675780
concepts[0].display_name	Loop-mediated isothermal amplification
concepts[1].id	https://openalex.org/C2780608908
concepts[1].level	5
concepts[1].score	0.8116424679756165
concepts[1].wikidata	https://www.wikidata.org/wiki/Q18394182
concepts[1].display_name	Reverse Transcription Loop-mediated Isothermal Amplification
concepts[2].id	https://openalex.org/C2777822432
concepts[2].level	2
concepts[2].score	0.6935902833938599
concepts[2].wikidata	https://www.wikidata.org/wiki/Q216681
concepts[2].display_name	In situ
concepts[3].id	https://openalex.org/C3007834351
concepts[3].level	5
concepts[3].score	0.6629582047462463
concepts[3].wikidata	https://www.wikidata.org/wiki/Q82069695
concepts[3].display_name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
concepts[4].id	https://openalex.org/C128489963
concepts[4].level	2
concepts[4].score	0.5578010678291321
concepts[4].wikidata	https://www.wikidata.org/wiki/Q1187724
concepts[4].display_name	Filtration (mathematics)
concepts[5].id	https://openalex.org/C3008058167
concepts[5].level	4
concepts[5].score	0.5153476595878601
concepts[5].wikidata	https://www.wikidata.org/wiki/Q84263196
concepts[5].display_name	Coronavirus disease 2019 (COVID-19)
concepts[6].id	https://openalex.org/C192562407
concepts[6].level	0
concepts[6].score	0.4560335874557495
concepts[6].wikidata	https://www.wikidata.org/wiki/Q228736
concepts[6].display_name	Materials science
concepts[7].id	https://openalex.org/C184670325
concepts[7].level	2
concepts[7].score	0.44746077060699463
concepts[7].wikidata	https://www.wikidata.org/wiki/Q512604
concepts[7].display_name	Loop (graph theory)
concepts[8].id	https://openalex.org/C3006700255
concepts[8].level	3
concepts[8].score	0.4316934645175934
concepts[8].wikidata	https://www.wikidata.org/wiki/Q81068910
concepts[8].display_name	2019-20 coronavirus outbreak
concepts[9].id	https://openalex.org/C159047783
concepts[9].level	1
concepts[9].score	0.4281498193740845
concepts[9].wikidata	https://www.wikidata.org/wiki/Q7215
concepts[9].display_name	Virology
concepts[10].id	https://openalex.org/C156719811
concepts[10].level	4
concepts[10].score	0.40413880348205566
concepts[10].wikidata	https://www.wikidata.org/wiki/Q28774
concepts[10].display_name	Reverse transcriptase
concepts[11].id	https://openalex.org/C185592680
concepts[11].level	0
concepts[11].score	0.2998230457305908
concepts[11].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[11].display_name	Chemistry
concepts[12].id	https://openalex.org/C86803240
concepts[12].level	0
concepts[12].score	0.2703976035118103
concepts[12].wikidata	https://www.wikidata.org/wiki/Q420
concepts[12].display_name	Biology
concepts[13].id	https://openalex.org/C49105822
concepts[13].level	3
concepts[13].score	0.13381564617156982
concepts[13].wikidata	https://www.wikidata.org/wiki/Q176996
concepts[13].display_name	Polymerase chain reaction
concepts[14].id	https://openalex.org/C71924100
concepts[14].level	0
concepts[14].score	0.13031736016273499
concepts[14].wikidata	https://www.wikidata.org/wiki/Q11190
concepts[14].display_name	Medicine
concepts[15].id	https://openalex.org/C54355233
concepts[15].level	1
concepts[15].score	0.10422390699386597
concepts[15].wikidata	https://www.wikidata.org/wiki/Q7162
concepts[15].display_name	Genetics
concepts[16].id	https://openalex.org/C104317684
concepts[16].level	2
concepts[16].score	0.0928167998790741
concepts[16].wikidata	https://www.wikidata.org/wiki/Q7187
concepts[16].display_name	Gene
concepts[17].id	https://openalex.org/C524204448
concepts[17].level	3
concepts[17].score	0.08168923854827881
concepts[17].wikidata	https://www.wikidata.org/wiki/Q788926
concepts[17].display_name	Infectious disease (medical specialty)
concepts[18].id	https://openalex.org/C116675565
concepts[18].level	2
concepts[18].score	0.0
concepts[18].wikidata	https://www.wikidata.org/wiki/Q3241045
concepts[18].display_name	Outbreak
concepts[19].id	https://openalex.org/C114614502
concepts[19].level	1
concepts[19].score	0.0
concepts[19].wikidata	https://www.wikidata.org/wiki/Q76592
concepts[19].display_name	Combinatorics
concepts[20].id	https://openalex.org/C142724271
concepts[20].level	1
concepts[20].score	0.0
concepts[20].wikidata	https://www.wikidata.org/wiki/Q7208
concepts[20].display_name	Pathology
concepts[21].id	https://openalex.org/C178790620
concepts[21].level	1
concepts[21].score	0.0
concepts[21].wikidata	https://www.wikidata.org/wiki/Q11351
concepts[21].display_name	Organic chemistry
concepts[22].id	https://openalex.org/C552990157
concepts[22].level	2
concepts[22].score	0.0
concepts[22].wikidata	https://www.wikidata.org/wiki/Q7430
concepts[22].display_name	DNA
concepts[23].id	https://openalex.org/C105795698
concepts[23].level	1
concepts[23].score	0.0
concepts[23].wikidata	https://www.wikidata.org/wiki/Q12483
concepts[23].display_name	Statistics
concepts[24].id	https://openalex.org/C33923547
concepts[24].level	0
concepts[24].score	0.0
concepts[24].wikidata	https://www.wikidata.org/wiki/Q395
concepts[24].display_name	Mathematics
concepts[25].id	https://openalex.org/C2779134260
concepts[25].level	2
concepts[25].score	0.0
concepts[25].wikidata	https://www.wikidata.org/wiki/Q12136
concepts[25].display_name	Disease
keywords[0].id	https://openalex.org/keywords/loop-mediated-isothermal-amplification
keywords[0].score	0.8345302939414978
keywords[0].display_name	Loop-mediated isothermal amplification
keywords[1].id	https://openalex.org/keywords/reverse-transcription-loop-mediated-isothermal-amplification
keywords[1].score	0.8116424679756165
keywords[1].display_name	Reverse Transcription Loop-mediated Isothermal Amplification
keywords[2].id	https://openalex.org/keywords/in-situ
keywords[2].score	0.6935902833938599
keywords[2].display_name	In situ
keywords[3].id	https://openalex.org/keywords/severe-acute-respiratory-syndrome-coronavirus-2
keywords[3].score	0.6629582047462463
keywords[3].display_name	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
keywords[4].id	https://openalex.org/keywords/filtration
keywords[4].score	0.5578010678291321
keywords[4].display_name	Filtration (mathematics)
keywords[5].id	https://openalex.org/keywords/coronavirus-disease-2019
keywords[5].score	0.5153476595878601
keywords[5].display_name	Coronavirus disease 2019 (COVID-19)
keywords[6].id	https://openalex.org/keywords/materials-science
keywords[6].score	0.4560335874557495
keywords[6].display_name	Materials science
keywords[7].id	https://openalex.org/keywords/loop
keywords[7].score	0.44746077060699463
keywords[7].display_name	Loop (graph theory)
keywords[8].id	https://openalex.org/keywords/2019-20-coronavirus-outbreak
keywords[8].score	0.4316934645175934
keywords[8].display_name	2019-20 coronavirus outbreak
keywords[9].id	https://openalex.org/keywords/virology
keywords[9].score	0.4281498193740845
keywords[9].display_name	Virology
keywords[10].id	https://openalex.org/keywords/reverse-transcriptase
keywords[10].score	0.40413880348205566
keywords[10].display_name	Reverse transcriptase
keywords[11].id	https://openalex.org/keywords/chemistry
keywords[11].score	0.2998230457305908
keywords[11].display_name	Chemistry
keywords[12].id	https://openalex.org/keywords/biology
keywords[12].score	0.2703976035118103
keywords[12].display_name	Biology
keywords[13].id	https://openalex.org/keywords/polymerase-chain-reaction
keywords[13].score	0.13381564617156982
keywords[13].display_name	Polymerase chain reaction
keywords[14].id	https://openalex.org/keywords/medicine
keywords[14].score	0.13031736016273499
keywords[14].display_name	Medicine
keywords[15].id	https://openalex.org/keywords/genetics
keywords[15].score	0.10422390699386597
keywords[15].display_name	Genetics
keywords[16].id	https://openalex.org/keywords/gene
keywords[16].score	0.0928167998790741
keywords[16].display_name	Gene
keywords[17].id	https://openalex.org/keywords/infectious-disease
keywords[17].score	0.08168923854827881
keywords[17].display_name	Infectious disease (medical specialty)
language	en
locations[0].id	doi:10.1021/acsomega.4c05784
locations[0].is_oa	True
locations[0].source.id	https://openalex.org/S4210239500
locations[0].source.issn	2470-1343
locations[0].source.type	journal
locations[0].source.is_oa	True
locations[0].source.issn_l	2470-1343
locations[0].source.is_core	True
locations[0].source.is_in_doaj	True
locations[0].source.display_name	ACS Omega
locations[0].source.host_organization	https://openalex.org/P4310320006
locations[0].source.host_organization_name	American Chemical Society
locations[0].source.host_organization_lineage	https://openalex.org/P4310320006
locations[0].source.host_organization_lineage_names	American Chemical Society
locations[0].license
locations[0].pdf_url
locations[0].version	publishedVersion
locations[0].raw_type	journal-article
locations[0].license_id
locations[0].is_accepted	True
locations[0].is_published	True
locations[0].raw_source_name	ACS Omega
locations[0].landing_page_url	https://doi.org/10.1021/acsomega.4c05784
locations[1].id	pmid:39372017
locations[1].is_oa	False
locations[1].source.id	https://openalex.org/S4306525036
locations[1].source.issn
locations[1].source.type	repository
locations[1].source.is_oa	False
locations[1].source.issn_l
locations[1].source.is_core	False
locations[1].source.is_in_doaj	False
locations[1].source.display_name	PubMed
locations[1].source.host_organization	https://openalex.org/I1299303238
locations[1].source.host_organization_name	National Institutes of Health
locations[1].source.host_organization_lineage	https://openalex.org/I1299303238
locations[1].license
locations[1].pdf_url
locations[1].version	publishedVersion
locations[1].raw_type
locations[1].license_id
locations[1].is_accepted	True
locations[1].is_published	True
locations[1].raw_source_name	ACS omega
locations[1].landing_page_url	https://pubmed.ncbi.nlm.nih.gov/39372017
locations[2].id	pmh:oai:doaj.org/article:d450b3169bef4deb8b65be3fa933a452
locations[2].is_oa	False
locations[2].source.id	https://openalex.org/S4306401280
locations[2].source.issn
locations[2].source.type	repository
locations[2].source.is_oa	False
locations[2].source.issn_l
locations[2].source.is_core	False
locations[2].source.is_in_doaj	False
locations[2].source.display_name	DOAJ (DOAJ: Directory of Open Access Journals)
locations[2].source.host_organization
locations[2].source.host_organization_name
locations[2].license
locations[2].pdf_url
locations[2].version	submittedVersion
locations[2].raw_type	article
locations[2].license_id
locations[2].is_accepted	False
locations[2].is_published	False
locations[2].raw_source_name	ACS Omega, Vol 9, Iss 39, Pp 40832-40840 (2024)
locations[2].landing_page_url	https://doaj.org/article/d450b3169bef4deb8b65be3fa933a452
locations[3].id	pmh:oai:pubmedcentral.nih.gov:11447726
locations[3].is_oa	True
locations[3].source.id	https://openalex.org/S2764455111
locations[3].source.issn
locations[3].source.type	repository
locations[3].source.is_oa	False
locations[3].source.issn_l
locations[3].source.is_core	False
locations[3].source.is_in_doaj	False
locations[3].source.display_name	PubMed Central
locations[3].source.host_organization	https://openalex.org/I1299303238
locations[3].source.host_organization_name	National Institutes of Health
locations[3].source.host_organization_lineage	https://openalex.org/I1299303238
locations[3].license
locations[3].pdf_url
locations[3].version	submittedVersion
locations[3].raw_type	Text
locations[3].license_id
locations[3].is_accepted	False
locations[3].is_published	False
locations[3].raw_source_name	ACS Omega
locations[3].landing_page_url	https://www.ncbi.nlm.nih.gov/pmc/articles/11447726
indexed_in	crossref, doaj, pubmed
authorships[0].author.id	https://openalex.org/A5055096243
authorships[0].author.orcid	https://orcid.org/0009-0009-0080-8636
authorships[0].author.display_name	Jacob Fry
authorships[0].countries	AU
authorships[0].affiliations[0].institution_ids	https://openalex.org/I165779595
authorships[0].affiliations[0].raw_affiliation_string	ARC Centre of Excellence in Exciton Science, The School of Chemistry, The University of Melbourne, Masson Rd, Parkville, Victoria 3010, Australia
authorships[0].affiliations[1].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[0].affiliations[1].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[0].institutions[0].id	https://openalex.org/I4210105123
authorships[0].institutions[0].ror	https://ror.org/016899r71
authorships[0].institutions[0].type	facility
authorships[0].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[0].institutions[0].country_code	AU
authorships[0].institutions[0].display_name	Peter Doherty Institute
authorships[0].institutions[1].id	https://openalex.org/I165779595
authorships[0].institutions[1].ror	https://ror.org/01ej9dk98
authorships[0].institutions[1].type	education
authorships[0].institutions[1].lineage	https://openalex.org/I165779595
authorships[0].institutions[1].country_code	AU
authorships[0].institutions[1].display_name	The University of Melbourne
authorships[0].author_position	first
authorships[0].raw_author_name	Jacob Fry
authorships[0].is_corresponding	False
authorships[0].raw_affiliation_strings	ARC Centre of Excellence in Exciton Science, The School of Chemistry, The University of Melbourne, Masson Rd, Parkville, Victoria 3010, Australia, Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[1].author.id	https://openalex.org/A5078874316
authorships[1].author.orcid	https://orcid.org/0000-0001-5751-8503
authorships[1].author.display_name	Jean Lee
authorships[1].countries	AU
authorships[1].affiliations[0].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[1].affiliations[0].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[1].institutions[0].id	https://openalex.org/I4210105123
authorships[1].institutions[0].ror	https://ror.org/016899r71
authorships[1].institutions[0].type	facility
authorships[1].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[1].institutions[0].country_code	AU
authorships[1].institutions[0].display_name	Peter Doherty Institute
authorships[1].institutions[1].id	https://openalex.org/I165779595
authorships[1].institutions[1].ror	https://ror.org/01ej9dk98
authorships[1].institutions[1].type	education
authorships[1].institutions[1].lineage	https://openalex.org/I165779595
authorships[1].institutions[1].country_code	AU
authorships[1].institutions[1].display_name	The University of Melbourne
authorships[1].author_position	middle
authorships[1].raw_author_name	Jean Y. H. Lee
authorships[1].is_corresponding	False
authorships[1].raw_affiliation_strings	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[2].author.id	https://openalex.org/A5087665241
authorships[2].author.orcid	https://orcid.org/0000-0003-2493-3465
authorships[2].author.display_name	Julie McAuley
authorships[2].countries	AU
authorships[2].affiliations[0].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[2].affiliations[0].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[2].institutions[0].id	https://openalex.org/I4210105123
authorships[2].institutions[0].ror	https://ror.org/016899r71
authorships[2].institutions[0].type	facility
authorships[2].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[2].institutions[0].country_code	AU
authorships[2].institutions[0].display_name	Peter Doherty Institute
authorships[2].institutions[1].id	https://openalex.org/I165779595
authorships[2].institutions[1].ror	https://ror.org/01ej9dk98
authorships[2].institutions[1].type	education
authorships[2].institutions[1].lineage	https://openalex.org/I165779595
authorships[2].institutions[1].country_code	AU
authorships[2].institutions[1].display_name	The University of Melbourne
authorships[2].author_position	middle
authorships[2].raw_author_name	Julie L. McAuley
authorships[2].is_corresponding	False
authorships[2].raw_affiliation_strings	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[3].author.id	https://openalex.org/A5105126126
authorships[3].author.orcid	https://orcid.org/0000-0002-0369-9641
authorships[3].author.display_name	Jessica L. Porter
authorships[3].countries	AU
authorships[3].affiliations[0].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[3].affiliations[0].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[3].institutions[0].id	https://openalex.org/I4210105123
authorships[3].institutions[0].ror	https://ror.org/016899r71
authorships[3].institutions[0].type	facility
authorships[3].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[3].institutions[0].country_code	AU
authorships[3].institutions[0].display_name	Peter Doherty Institute
authorships[3].institutions[1].id	https://openalex.org/I165779595
authorships[3].institutions[1].ror	https://ror.org/01ej9dk98
authorships[3].institutions[1].type	education
authorships[3].institutions[1].lineage	https://openalex.org/I165779595
authorships[3].institutions[1].country_code	AU
authorships[3].institutions[1].display_name	The University of Melbourne
authorships[3].author_position	middle
authorships[3].raw_author_name	Jessica L. Porter
authorships[3].is_corresponding	False
authorships[3].raw_affiliation_strings	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[4].author.id	https://openalex.org/A5017416740
authorships[4].author.orcid	https://orcid.org/0000-0001-6982-8074
authorships[4].author.display_name	Ian R. Monk
authorships[4].countries	AU
authorships[4].affiliations[0].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[4].affiliations[0].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[4].institutions[0].id	https://openalex.org/I4210105123
authorships[4].institutions[0].ror	https://ror.org/016899r71
authorships[4].institutions[0].type	facility
authorships[4].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[4].institutions[0].country_code	AU
authorships[4].institutions[0].display_name	Peter Doherty Institute
authorships[4].institutions[1].id	https://openalex.org/I165779595
authorships[4].institutions[1].ror	https://ror.org/01ej9dk98
authorships[4].institutions[1].type	education
authorships[4].institutions[1].lineage	https://openalex.org/I165779595
authorships[4].institutions[1].country_code	AU
authorships[4].institutions[1].display_name	The University of Melbourne
authorships[4].author_position	middle
authorships[4].raw_author_name	Ian R. Monk
authorships[4].is_corresponding	False
authorships[4].raw_affiliation_strings	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[5].author.id	https://openalex.org/A5111333320
authorships[5].author.orcid
authorships[5].author.display_name	Samuel T. Martin
authorships[5].countries	AU
authorships[5].affiliations[0].institution_ids	https://openalex.org/I165779595
authorships[5].affiliations[0].raw_affiliation_string	Department of Biomedical Engineering, The University of Melbourne, Building 261/203 Bouverie St, Carlton, Victoria 3053, Australia
authorships[5].institutions[0].id	https://openalex.org/I165779595
authorships[5].institutions[0].ror	https://ror.org/01ej9dk98
authorships[5].institutions[0].type	education
authorships[5].institutions[0].lineage	https://openalex.org/I165779595
authorships[5].institutions[0].country_code	AU
authorships[5].institutions[0].display_name	The University of Melbourne
authorships[5].author_position	middle
authorships[5].raw_author_name	Samuel T. Martin
authorships[5].is_corresponding	False
authorships[5].raw_affiliation_strings	Department of Biomedical Engineering, The University of Melbourne, Building 261/203 Bouverie St, Carlton, Victoria 3053, Australia
authorships[6].author.id	https://openalex.org/A5078859691
authorships[6].author.orcid	https://orcid.org/0000-0001-5382-9718
authorships[6].author.display_name	David J. Collins
authorships[6].countries	AU
authorships[6].affiliations[0].institution_ids	https://openalex.org/I165779595
authorships[6].affiliations[0].raw_affiliation_string	Graeme Clarke Institute, The University of Melbourne, Chemical Engineering 2 Building 167, Parkville, Victoria 3010, Australia
authorships[6].affiliations[1].institution_ids	https://openalex.org/I165779595
authorships[6].affiliations[1].raw_affiliation_string	Department of Biomedical Engineering, The University of Melbourne, Building 261/203 Bouverie St, Carlton, Victoria 3053, Australia
authorships[6].institutions[0].id	https://openalex.org/I165779595
authorships[6].institutions[0].ror	https://ror.org/01ej9dk98
authorships[6].institutions[0].type	education
authorships[6].institutions[0].lineage	https://openalex.org/I165779595
authorships[6].institutions[0].country_code	AU
authorships[6].institutions[0].display_name	The University of Melbourne
authorships[6].author_position	middle
authorships[6].raw_author_name	David J. Collins
authorships[6].is_corresponding	False
authorships[6].raw_affiliation_strings	Department of Biomedical Engineering, The University of Melbourne, Building 261/203 Bouverie St, Carlton, Victoria 3053, Australia, Graeme Clarke Institute, The University of Melbourne, Chemical Engineering 2 Building 167, Parkville, Victoria 3010, Australia
authorships[7].author.id	https://openalex.org/A5090534466
authorships[7].author.orcid
authorships[7].author.display_name	Gregory J. Barbante
authorships[7].countries	AU
authorships[7].affiliations[0].institution_ids	https://openalex.org/I1303474014
authorships[7].affiliations[0].raw_affiliation_string	Defence Science and Technology Group, Australian Department of Defence, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia
authorships[7].institutions[0].id	https://openalex.org/I1303474014
authorships[7].institutions[0].ror	https://ror.org/05ddrvt52
authorships[7].institutions[0].type	government
authorships[7].institutions[0].lineage	https://openalex.org/I1303474014, https://openalex.org/I2801453606, https://openalex.org/I3139952251
authorships[7].institutions[0].country_code	AU
authorships[7].institutions[0].display_name	Defence Science and Technology Group
authorships[7].author_position	middle
authorships[7].raw_author_name	Gregory J. Barbante
authorships[7].is_corresponding	False
authorships[7].raw_affiliation_strings	Defence Science and Technology Group, Australian Department of Defence, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia
authorships[8].author.id	https://openalex.org/A5036035956
authorships[8].author.orcid
authorships[8].author.display_name	N.J. FitzGerald
authorships[8].countries	AU
authorships[8].affiliations[0].institution_ids	https://openalex.org/I1303474014
authorships[8].affiliations[0].raw_affiliation_string	Defence Science and Technology Group, Australian Department of Defence, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia
authorships[8].institutions[0].id	https://openalex.org/I1303474014
authorships[8].institutions[0].ror	https://ror.org/05ddrvt52
authorships[8].institutions[0].type	government
authorships[8].institutions[0].lineage	https://openalex.org/I1303474014, https://openalex.org/I2801453606, https://openalex.org/I3139952251
authorships[8].institutions[0].country_code	AU
authorships[8].institutions[0].display_name	Defence Science and Technology Group
authorships[8].author_position	middle
authorships[8].raw_author_name	Nicholas J. Fitzgerald
authorships[8].is_corresponding	False
authorships[8].raw_affiliation_strings	Defence Science and Technology Group, Australian Department of Defence, 506 Lorimer Street, Fishermans Bend, Victoria 3207, Australia
authorships[9].author.id	https://openalex.org/A5058500554
authorships[9].author.orcid	https://orcid.org/0000-0003-0150-123X
authorships[9].author.display_name	Timothy P. Stinear
authorships[9].countries	AU
authorships[9].affiliations[0].institution_ids	https://openalex.org/I165779595, https://openalex.org/I4210105123
authorships[9].affiliations[0].raw_affiliation_string	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
authorships[9].institutions[0].id	https://openalex.org/I4210105123
authorships[9].institutions[0].ror	https://ror.org/016899r71
authorships[9].institutions[0].type	facility
authorships[9].institutions[0].lineage	https://openalex.org/I1320473756, https://openalex.org/I165779595, https://openalex.org/I4210105123, https://openalex.org/I4210158168
authorships[9].institutions[0].country_code	AU
authorships[9].institutions[0].display_name	Peter Doherty Institute
authorships[9].institutions[1].id	https://openalex.org/I165779595
authorships[9].institutions[1].ror	https://ror.org/01ej9dk98
authorships[9].institutions[1].type	education
authorships[9].institutions[1].lineage	https://openalex.org/I165779595
authorships[9].institutions[1].country_code	AU
authorships[9].institutions[1].display_name	The University of Melbourne
authorships[9].author_position	last
authorships[9].raw_author_name	Timothy P. Stinear
authorships[9].is_corresponding	False
authorships[9].raw_affiliation_strings	Department of Microbiology and Immunology, The Doherty Institute for Infection and Immunity, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://doi.org/10.1021/acsomega.4c05784
open_access.oa_status	gold
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T11393
primary_topic.field.id	https://openalex.org/fields/22
primary_topic.field.display_name	Engineering
primary_topic.score	0.9998999834060669
primary_topic.domain.id	https://openalex.org/domains/3
primary_topic.domain.display_name	Physical Sciences
primary_topic.subfield.id	https://openalex.org/subfields/2204
primary_topic.subfield.display_name	Biomedical Engineering
primary_topic.display_name	Biosensors and Analytical Detection
related_works	https://openalex.org/W2971084591, https://openalex.org/W3142487381, https://openalex.org/W2048217543, https://openalex.org/W2033708183, https://openalex.org/W1976841726, https://openalex.org/W2120551273, https://openalex.org/W2411499991, https://openalex.org/W2035862153, https://openalex.org/W15712792, https://openalex.org/W2114778199
cited_by_count	2
counts_by_year[0].year	2025
counts_by_year[0].cited_by_count	2
locations_count	4
best_oa_location.id	doi:10.1021/acsomega.4c05784
best_oa_location.is_oa	True
best_oa_location.source.id	https://openalex.org/S4210239500
best_oa_location.source.issn	2470-1343
best_oa_location.source.type	journal
best_oa_location.source.is_oa	True
best_oa_location.source.issn_l	2470-1343
best_oa_location.source.is_core	True
best_oa_location.source.is_in_doaj	True
best_oa_location.source.display_name	ACS Omega
best_oa_location.source.host_organization	https://openalex.org/P4310320006
best_oa_location.source.host_organization_name	American Chemical Society
best_oa_location.source.host_organization_lineage	https://openalex.org/P4310320006
best_oa_location.source.host_organization_lineage_names	American Chemical Society
best_oa_location.license
best_oa_location.pdf_url
best_oa_location.version	publishedVersion
best_oa_location.raw_type	journal-article
best_oa_location.license_id
best_oa_location.is_accepted	True
best_oa_location.is_published	True
best_oa_location.raw_source_name	ACS Omega
best_oa_location.landing_page_url	https://doi.org/10.1021/acsomega.4c05784
primary_location.id	doi:10.1021/acsomega.4c05784
primary_location.is_oa	True
primary_location.source.id	https://openalex.org/S4210239500
primary_location.source.issn	2470-1343
primary_location.source.type	journal
primary_location.source.is_oa	True
primary_location.source.issn_l	2470-1343
primary_location.source.is_core	True
primary_location.source.is_in_doaj	True
primary_location.source.display_name	ACS Omega
primary_location.source.host_organization	https://openalex.org/P4310320006
primary_location.source.host_organization_name	American Chemical Society
primary_location.source.host_organization_lineage	https://openalex.org/P4310320006
primary_location.source.host_organization_lineage_names	American Chemical Society
primary_location.license
primary_location.pdf_url
primary_location.version	publishedVersion
primary_location.raw_type	journal-article
primary_location.license_id
primary_location.is_accepted	True
primary_location.is_published	True
primary_location.raw_source_name	ACS Omega
primary_location.landing_page_url	https://doi.org/10.1021/acsomega.4c05784
publication_date	2024-09-20
publication_year	2024
referenced_works	https://openalex.org/W4220877703, https://openalex.org/W2105275554, https://openalex.org/W1979974453, https://openalex.org/W2752427063, https://openalex.org/W2149136689, https://openalex.org/W2006882476, https://openalex.org/W4297107102, https://openalex.org/W2065098811, https://openalex.org/W3127615546, https://openalex.org/W3200727692, https://openalex.org/W2885281620, https://openalex.org/W2192426732, https://openalex.org/W2149622631, https://openalex.org/W2218287097, https://openalex.org/W3121985569, https://openalex.org/W2783863479, https://openalex.org/W3159703094, https://openalex.org/W2916501589, https://openalex.org/W2621862861, https://openalex.org/W3047013814, https://openalex.org/W3108001574, https://openalex.org/W3017281110, https://openalex.org/W3017152347, https://openalex.org/W3048028928, https://openalex.org/W4229041927, https://openalex.org/W4282922862, https://openalex.org/W3081542135, https://openalex.org/W2135483495, https://openalex.org/W3196765125, https://openalex.org/W3048164837, https://openalex.org/W3016207602, https://openalex.org/W4237422783, https://openalex.org/W3204646187, https://openalex.org/W3030968929, https://openalex.org/W3123909269, https://openalex.org/W3013374708, https://openalex.org/W2256756668, https://openalex.org/W3195777720, https://openalex.org/W3085854736, https://openalex.org/W2418180773, https://openalex.org/W2119289482, https://openalex.org/W4223578888, https://openalex.org/W3122815252, https://openalex.org/W2071500103
referenced_works_count	44
abstract_inverted_index.2	67
abstract_inverted_index.a	82
abstract_inverted_index.We	80
abstract_inverted_index.an	35, 134
abstract_inverted_index.in	9
abstract_inverted_index.is	34, 133
abstract_inverted_index.of	12, 20, 27, 61, 77, 84, 136
abstract_inverted_index.on	71, 97, 113
abstract_inverted_index.to	17, 88
abstract_inverted_index.we	49, 108
abstract_inverted_index.50%	120
abstract_inverted_index.The	0
abstract_inverted_index.and	15, 105, 132
abstract_inverted_index.for	38, 58, 75, 94
abstract_inverted_index.has	6
abstract_inverted_index.led	16
abstract_inverted_index.per	127
abstract_inverted_index.run	70
abstract_inverted_index.the	10, 18, 24, 90
abstract_inverted_index.use	81
abstract_inverted_index.0.10	119
abstract_inverted_index.2019	3
abstract_inverted_index.SCV2	101, 126
abstract_inverted_index.dose	124
abstract_inverted_index.more	138
abstract_inverted_index.show	109
abstract_inverted_index.than	140
abstract_inverted_index.that	22, 110
abstract_inverted_index.(3600	129
abstract_inverted_index.Here,	48
abstract_inverted_index.Using	100
abstract_inverted_index.acute	63
abstract_inverted_index.fiber	115
abstract_inverted_index.field	11
abstract_inverted_index.glass	114
abstract_inverted_index.limit	45
abstract_inverted_index.order	135
abstract_inverted_index.risk,	41
abstract_inverted_index.virus	32
abstract_inverted_index.(SCV2)	69
abstract_inverted_index.E-gene	130
abstract_inverted_index.Severe	62
abstract_inverted_index.assays	57
abstract_inverted_index.design	83
abstract_inverted_index.liquid	102
abstract_inverted_index.permit	23
abstract_inverted_index.tissue	121
abstract_inverted_index.RT-LAMP	96, 112
abstract_inverted_index.agents.	30
abstract_inverted_index.copies)	131
abstract_inverted_index.culture	122
abstract_inverted_index.current	43
abstract_inverted_index.disease	2
abstract_inverted_index.explore	50
abstract_inverted_index.filters	73, 116
abstract_inverted_index.methods	44
abstract_inverted_index.one-pot	59
abstract_inverted_index.optimal	91
abstract_inverted_index.reverse	51
abstract_inverted_index.running	95
abstract_inverted_index.systems	21
abstract_inverted_index.Airborne	31
abstract_inverted_index.RT-LAMP.	142
abstract_inverted_index.additive	92
abstract_inverted_index.airborne	28, 78
abstract_inverted_index.although	42
abstract_inverted_index.approach	37
abstract_inverted_index.detected	118
abstract_inverted_index.emerging	36
abstract_inverted_index.exposure	40
abstract_inverted_index.filters.	99
abstract_inverted_index.membrane	72, 98
abstract_inverted_index.pandemic	5
abstract_inverted_index.reaction	128
abstract_inverted_index.reliably	117
abstract_inverted_index.spike-in	103
abstract_inverted_index.suitable	74
abstract_inverted_index.syndrome	65
abstract_inverted_index.viruses.	79
abstract_inverted_index.(RT-LAMP)	56
abstract_inverted_index.detection	33, 60
abstract_inverted_index.establish	89
abstract_inverted_index.framework	87
abstract_inverted_index.magnitude	137
abstract_inverted_index.molecular	13
abstract_inverted_index.sensitive	139
abstract_inverted_index.(COVID-19)	4
abstract_inverted_index.autonomous	25
abstract_inverted_index.detection,	107
abstract_inverted_index.infectious	29, 123
abstract_inverted_index.innovation	8
abstract_inverted_index.isothermal	54
abstract_inverted_index.single-pot	111
abstract_inverted_index.Coronavirus	1, 66
abstract_inverted_index.composition	93
abstract_inverted_index.determining	39
abstract_inverted_index.diagnostics	14
abstract_inverted_index.experiments	85, 104
abstract_inverted_index.exploration	19
abstract_inverted_index.respiratory	64
abstract_inverted_index.statistical	86
abstract_inverted_index.timeliness.	47
abstract_inverted_index.(SARS-CoV-2)	68
abstract_inverted_index.conventional	141
abstract_inverted_index.fluorescence	106
abstract_inverted_index.intervention	46
abstract_inverted_index.supercharged	7
abstract_inverted_index.amplification	55
abstract_inverted_index.loop-mediated	53
abstract_inverted_index.transcription	52
abstract_inverted_index.identification	26
abstract_inverted_index.(TCID<sub>50</sub>)	125
abstract_inverted_index.micro-air-filtration	76
cited_by_percentile_year.max	97
cited_by_percentile_year.min	95
countries_distinct_count	1
institutions_distinct_count	10
sustainable_development_goals[0].id	https://metadata.un.org/sdg/6
sustainable_development_goals[0].score	0.5600000023841858
sustainable_development_goals[0].display_name	Clean water and sanitation
citation_normalized_percentile.value	0.6295459
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False