Optogenetic Control of Airway Cholinergic NeuronsIn Vivo Article Swipe
Alexandra Pincus
,
Sweta Adhikary
,
Katie M. Lebold
,
A.D. Fryer
,
David B. Jacoby
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1165/rcmb.2019-0378ma
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1165/rcmb.2019-0378ma
Dysregulation of airway nerves leads to airway hyperreactivity, a hallmark of asthma. Although changes to nerve density and phenotype have been described in asthma, the relevance of these changes to nerve function has not been investigated due to anatomical limitations where afferent and efferent nerves run in the same nerve trunk, making it difficult to assess their independent contributions. We developed a unique and accessible system to activate specific airway nerves to investigate their function in mouse models of airway disease. We describe a method to specifically activate cholinergic neurons using light, resulting in immediate, measurable increases in airway inflation pressure and decreases in heart rate. Expression of light-activated channelrhodopsin 2 in these neurons is governed by Cre expression under the endogenous choline acetyltransferase promoter, and we describe a method to decrease variability in channelrhodopsin expression in future experiments. Optogenetic activation of specific subsets of airway neurons will be useful for studying the functional relevance of other observed changes, such as changes to nerve morphology and protein expression, across many airway diseases, and may be used to study the function of subpopulations of autonomic neurons in lungs and other organs.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1165/rcmb.2019-0378ma
- OA Status
- green
- Cited By
- 13
- References
- 27
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2996803232
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2996803232Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1165/rcmb.2019-0378maDigital Object Identifier
- Title
-
Optogenetic Control of Airway Cholinergic NeuronsIn VivoWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-01-03Full publication date if available
- Authors
-
Alexandra Pincus, Sweta Adhikary, Katie M. Lebold, A.D. Fryer, David B. JacobyList of authors in order
- Landing page
-
https://doi.org/10.1165/rcmb.2019-0378maPublisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.ncbi.nlm.nih.gov/pmc/articles/7110977Direct OA link when available
- Concepts
-
Optogenetics, Channelrhodopsin, Cholinergic, Neuroscience, Airway, Choline acetyltransferase, Cholinergic neuron, Vagus nerve, Medicine, Efferent, Biology, Afferent, Anesthesia, StimulationTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
13Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 4, 2023: 4, 2022: 1, 2021: 2Per-year citation counts (last 5 years)
- References (count)
-
27Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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