Orthogonal translation components for the in vivo incorporation of unnatural amino acids Article Swipe

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Translation (biology) Amino acid In vivo Chemistry Computer science Computational biology Biochemistry Biology Genetics Messenger RNA Gene

Peter G. Schultz , Lital Alfonta , Johnathan Chittuluru , Alexander Deiters , Dan Groff , Daniel Summerer , Meng -Lin Tsao , Jiangyun Wang , Wu Ning , Jianming Xie , Huaqiang Zeng , Mohammad R. Seyedsayamdost , James Turner ·

YOU? · · 2023 · Open Access · · OA: W4300309107

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as <i>E. coli</i>, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.