Pair‐Feeding Study Designs Can Create Biases and Inflate Type I Error Rates: A Simulation Study Article Swipe
Wasiuddin Najam
,
Bret Rust
,
Lilian Golzarri‐Arroyo
,
Joseph G. Ibrahim
,
David B. Allison
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/oby.70079
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/oby.70079
Objective Pair‐feeding is a study design element where one group's food intake is provided to another group to assess whether a treatment effect is independent of food intake. Investigators often assume equivalent food intake across experimental conditions and exclude it from the statistical analysis. However, the impact of this practice on type I error (T1Er) rates has not been quantified. Methods We conducted a Monte Carlo simulation in which animals were assigned baseline weights and food intakes, then randomized to non‐pair‐fed or pair‐fed groups. Daily food intake for both groups was initially drawn from the baseline food intake distribution. For pair‐fed animals, food intake was truncated if it exceeded the previous day's intake of the matched non‐pair‐fed animal (individual pair‐feeding) or the group's average (group pair‐feeding). Weight changes were calculated as a function of food intake, and final weight change was analyzed with and without adjusting for mean food intake. Results Both individual and group pair‐feeding inflated T1Er rates ranging from 0.12 to 0.71 in unadjusted models. However, adjustment for food intake reduced error rates to around 0.05. Conclusions Under some circumstances, pair‐feeding designs can inflate T1Er rates. Investigators can mitigate this inflation by adjusting the analyses for food intake.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/oby.70079
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/oby.70079
- OA Status
- hybrid
- References
- 20
- OpenAlex ID
- https://openalex.org/W4416678411
All OpenAlex metadata
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https://doi.org/10.1002/oby.70079Digital Object Identifier
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Pair‐Feeding Study Designs Can Create Biases and Inflate Type I Error Rates: A Simulation StudyWork title
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articleOpenAlex work type
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enPrimary language
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2025Year of publication
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2025-11-24Full publication date if available
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Wasiuddin Najam, Bret Rust, Lilian Golzarri‐Arroyo, Joseph G. Ibrahim, David B. AllisonList of authors in order
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https://doi.org/10.1002/oby.70079Publisher landing page
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/oby.70079Direct link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/oby.70079Direct OA link when available
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0Total citation count in OpenAlex
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20Number of works referenced by this work
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