PAMPs of Piscirickettsia salmonis Trigger the Transcription of Genes Involved in Nutritional Immunity in a Salmon Macrophage-Like Cell Line Article Swipe
D. Martínez
,
Cristian Oliver
,
Natacha Santibáñez
,
José Leonardo Coronado
,
R. Oyarzún
,
Ricardo Enríquez
,
Luis Vargas‐Chacoff
,
Alex Romero
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fimmu.2022.849752
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fimmu.2022.849752
The innate immune system can limit the growth of invading pathogens by depleting micronutrients at a cellular and tissue level. However, it is not known whether nutrient depletion mechanisms discriminate between living pathogens (which require nutrients) and pathogen-associated molecular patterns (PAMPs) (which do not). We stimulated SHK-1 cells with different PAMPs (outer membrane vesicles of Piscirickettsia salmonis “OMVs”, protein extract of P. salmonis “TP” and lipopolysaccharides of P. salmonis “LPS”) isolated from P. salmonis and evaluated transcriptional changes in nutritional immunity associated genes. Our experimental treatments were: Control (SHK-1 stimulated with bacterial culture medium), OMVs (SHK-1 stimulated with 1μg of outer membrane vesicles), TP (SHK-1 stimulated with 1μg of total protein extract) and LPS (SHK-1 stimulated with 1μg of lipopolysaccharides). Cells were sampled at 15-, 30-, 60- and 120-minutes post-stimulation. We detected increased transcription of zip8 , zip14 , irp1 , irp2 and tfr1 in all three experimental conditions and increased transcription of dmt1 in cells stimulated with OMVs and TP, but not LPS. Additionally, we observed generally increased transcription of ireg-1, il-6 , hamp , irp1 , ft-h and ft-m in all three experimental conditions, but we also detected decreased transcription of these markers in cells stimulated with TP and LPS at specific time points. Our results demonstrate that SHK-1 cells stimulated with P. salmonis PAMPs increase transcription of markers involved in the transport, uptake, storage and regulation of micronutrients such as iron, manganese and zinc.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fimmu.2022.849752
- https://www.frontiersin.org/articles/10.3389/fimmu.2022.849752/pdf
- OA Status
- gold
- Cited By
- 11
- References
- 44
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4223933545
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4223933545Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fimmu.2022.849752Digital Object Identifier
- Title
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PAMPs of Piscirickettsia salmonis Trigger the Transcription of Genes Involved in Nutritional Immunity in a Salmon Macrophage-Like Cell LineWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-04-14Full publication date if available
- Authors
-
D. Martínez, Cristian Oliver, Natacha Santibáñez, José Leonardo Coronado, R. Oyarzún, Ricardo Enríquez, Luis Vargas‐Chacoff, Alex RomeroList of authors in order
- Landing page
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https://doi.org/10.3389/fimmu.2022.849752Publisher landing page
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https://www.frontiersin.org/articles/10.3389/fimmu.2022.849752/pdfDirect link to full text PDF
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://www.frontiersin.org/articles/10.3389/fimmu.2022.849752/pdfDirect OA link when available
- Concepts
-
Macrophage, Immunity, Biology, Gene, Innate immune system, Virology, Immunology, Immune system, Genetics, In vitroTop concepts (fields/topics) attached by OpenAlex
- Cited by
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11Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2024: 1, 2023: 7Per-year citation counts (last 5 years)
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44Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.lipopolysaccharides). | 119 |
| cited_by_percentile_year.max | 99 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5023953501, https://openalex.org/A5053304924, https://openalex.org/A5012783603 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 8 |
| corresponding_institution_ids | https://openalex.org/I16465266, https://openalex.org/I4210091755, https://openalex.org/I4210155123, https://openalex.org/I4210167034 |
| citation_normalized_percentile.value | 0.73581499 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |