Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona Article Swipe

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Amplicon Enterovirus Genotype Virology Capsid Biology Molecular epidemiology Epidemiology Amplicon sequencing Wastewater Virus Genetics Medicine Polymerase chain reaction Gene Internal medicine Environmental science Environmental engineering 16S ribosomal RNA

Temitope O. C. Faleye , Erin M. Driver , Devin A. Bowes , Sangeet Adhikari , D. Adams , Arvind Varsani , Rolf U. Halden , Matthew Scotch ·

YOU? · · 2021 · Open Access · · DOI: https://doi.org/10.3390/v13010074 · OA: W3120116204

We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have been circulating for over two years, but its sequence has not been documented in that period. As the SARS-CoV-2 pandemic has resulted in changes in health-seeking behavior and overwhelmed pathogen diagnostics, our findings highlight the importance of wastewater-based epidemiology (WBE ) as an early warning system for virus surveillance.