Pectin Nanostructure Visualization by Atomic Force Microscopy Article Swipe
Sara Pos�
,
Candelas Paniagua
,
Andrew R. Kirby
,
A. Patrick Gunning
,
Victor J. Morris
,
Miguel A. Quesada
,
José A. Mercado
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.21769/bioprotoc.1598
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.21769/bioprotoc.1598
Pectins, complex polysaccharides rich in galacturonic acid, are a major component of primary plant cell walls. These macromolecules regulate cell wall porosity and intercellular adhesion, being important in the control of cell expansion and differentiation through their effect on the rheological properties of the cell wall. In fruits, pectin disassembly during ripening is one the main event leading to textural changes and softening. Changes in pectic polymer size, composition and structure have been studied by conventional techniques, most of them relying on bulk analysis of a population of polysaccharides but studies of detailed structure of isolated polymer chains are scarce (Paniagua et al., 2014). Atomic force microscopy (AFM) is a versatile and powerful technique able to analyze force measurements, as well as to visualize roughness of biological samples at nanoscale (Morris et al., 2010). Using this technique, recent research has found a close relationship between pectin nanostructural complexity and texture and postharvest behavior in several fruits (Liu and Cheng, 2011; Cybulska et al., 2014; Posé et al., 2015). Here, we describe an AFM procedure to topographically visualize pectic polymers from fruit cell wall extracts that has successfully been used in the study of strawberry ripening (Posé et al., 2012; Posé et al., 2015). Thus, from AFM images the 3D structural analysis of isolated chains (length, height, and branch pattern) can be resolved at high magnification and with minimal sample preparation. A full description of AFM fundamentals and the different sampling modes are described in Morris et al. (2010).
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.21769/bioprotoc.1598
- https://bio-protocol.org/pdf/Bio-protocol1598.pdf
- OA Status
- bronze
- Cited By
- 1
- References
- 9
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2527217935
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https://openalex.org/W2527217935Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21769/bioprotoc.1598Digital Object Identifier
- Title
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Pectin Nanostructure Visualization by Atomic Force MicroscopyWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2015Year of publication
- Publication date
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2015-01-01Full publication date if available
- Authors
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Sara Pos�, Candelas Paniagua, Andrew R. Kirby, A. Patrick Gunning, Victor J. Morris, Miguel A. Quesada, José A. MercadoList of authors in order
- Landing page
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https://doi.org/10.21769/bioprotoc.1598Publisher landing page
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https://bio-protocol.org/pdf/Bio-protocol1598.pdfDirect link to full text PDF
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://bio-protocol.org/pdf/Bio-protocol1598.pdfDirect OA link when available
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Pectin, Nanostructure, Polymer, Texture (cosmology), Atomic force microscopy, Chemistry, Cell wall, Population, Nanoscopic scale, Nanotechnology, Materials science, Composite material, Food science, Biochemistry, Demography, Image (mathematics), Artificial intelligence, Sociology, Computer scienceTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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