Physicochemical Properties of Extracellular Polymeric Substances Produced by Three Bacterial Isolates From Biofouled Reverse Osmosis Membranes Article Swipe
Zahid Ur Rehman
,
Johannes S. Vrouwenvelder
,
Pascal E. Saikaly
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2021.668761
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2021.668761
This work describes the chemical composition of extracellular polymeric substances (EPS) produced by three bacteria (RO1, RO2, and RO3) isolated from a biofouled reverse osmosis (RO) membrane. We isolated pure cultures of three bacterial strains from a 7-year-old biofouled RO module that was used in a full-scale seawater treatment plant. All the bacterial strains showed similar growth rates, biofilm formation, and produced similar quantities of proteins and polysaccharides. The gel permeation chromatography showed that the EPS produced by all the strains has a high molecular weight; however, the EPS produced by strains RO1 and RO3 showed the highest molecular weight. Fourier Transform Infrared Spectroscopy (FTIR), Proton Nuclear Magnetic Resonance ( 1 H NMR), and Carbon NMR ( 13 C NMR) were used for a detailed characterization of the EPS. These physicochemical analyses allowed us to identify features of EPS that are important for biofilm formation. FTIR analysis indicated the presence of α-1,4 glycosidic linkages (920 cm –1 ) and amide II (1,550 cm –1 ) in the EPS, the presence of which has been correlated with the fouling potential of bacteria. The presence of α-glycoside linkages was further confirmed by 13 C NMR analysis. The 13 C NMR analysis also showed that the EPS produced by these bacteria is chemically similar to foulants obtained from biofouled RO membranes in previous studies. Therefore, our results support the hypothesis that the majority of substances that cause fouling on RO membranes originate from bacteria. Investigation using 1 H NMR showed that the EPS contained a high abundance of hydrophobic compounds, and these compounds can lead to flux decline in the membrane processes. Genome sequencing of the isolates showed that they represent novel species of bacteria belonging to the genus Bacillus . Examination of genomes showed that these bacteria carry carbohydrates-active enzymes that play a role in the production of polysaccharides. Further genomic studies allowed us to identify proteins involved in the biosynthesis of EPS and flagella involved in biofilm formation. These analyses provide a glimpse into the physicochemical properties of EPS found on the RO membrane. This knowledge can be useful in the rational design of biofilm control treatments for the RO membrane.
Related Topics
Concepts
Chemistry
Extracellular polymeric substance
Membrane
Gel permeation chromatography
Bacteria
Reverse osmosis
Chromatography
Fourier transform infrared spectroscopy
Nuclear magnetic resonance spectroscopy
Polysaccharide
Biofilm
Nuclear chemistry
Organic chemistry
Biochemistry
Chemical engineering
Biology
Polymer
Engineering
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fmicb.2021.668761
- OA Status
- gold
- Cited By
- 56
- References
- 86
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3178288844
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3178288844Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fmicb.2021.668761Digital Object Identifier
- Title
-
Physicochemical Properties of Extracellular Polymeric Substances Produced by Three Bacterial Isolates From Biofouled Reverse Osmosis MembranesWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2021Year of publication
- Publication date
-
2021-07-13Full publication date if available
- Authors
-
Zahid Ur Rehman, Johannes S. Vrouwenvelder, Pascal E. SaikalyList of authors in order
- Landing page
-
https://doi.org/10.3389/fmicb.2021.668761Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.3389/fmicb.2021.668761Direct OA link when available
- Concepts
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Chemistry, Extracellular polymeric substance, Membrane, Gel permeation chromatography, Bacteria, Reverse osmosis, Chromatography, Fourier transform infrared spectroscopy, Nuclear magnetic resonance spectroscopy, Polysaccharide, Biofilm, Nuclear chemistry, Organic chemistry, Biochemistry, Chemical engineering, Biology, Polymer, Engineering, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
56Total citation count in OpenAlex
- Citations by year (recent)
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2025: 26, 2024: 15, 2023: 9, 2022: 6Per-year citation counts (last 5 years)
- References (count)
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86Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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