Point-of-Care Diagnosis of Malaria Using a Simple, Purification-Free DNA Extraction Method Coupled with Loop-Mediated Isothermal Amplification-Lateral Flow Article Swipe
Meng Yee Lai
,
Lee Phone Youth Zen
,
Mohd Hafizi Abdul Hamid
,
Jenarun Jelip
,
Rose Nani Mudin
,
Vun Jan Shui Ivan
,
Lee Ngie Ping Francis
,
Izreena Saihidi
,
Yee Ling Lau
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/tropicalmed8040199
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/tropicalmed8040199
We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detect Plasmodium knowlesi, P. vivax, P. falciparum, and Plasmodium genus (for P. malariae and P. ovale). Through the capillary effect, the results can be observed by the red band signal on the test and control lines within 5 min. The developed multiplex LAMP-LF was tested with 86 clinical blood samples on-site at Hospital Kapit, Sarawak, Malaysia. By using microscopy as the reference method, the multiplex LAMP-LF showed 100% sensitivity (95% confidence interval (CI): 91.4 to 100.00%) and 97.8% specificity (95% CI: 88.2% to 99.9%). The high sensitivity and specificity of multiplex LAMP-LF make it ideal for use as a point-of-care diagnostic tool. The simple and purification-free DNA extraction protocol can be employed as an alternative DNA extraction method for malaria diagnosis in resource-limited settings. By combining the simple DNA extraction protocol and multiplex LAMP-LF approach, we aim to develop a simple-to-handle and easy-to-read molecular diagnostic tool for malaria in both laboratory and on-site settings.
Related Topics
Concepts
Loop-mediated isothermal amplification
DNA extraction
Multiplex
Diagnosis of malaria
Malaria
DNA
Biology
Plasmodium falciparum
Chromatography
Polymerase chain reaction
Chemistry
Bioinformatics
Immunology
Genetics
Gene
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/tropicalmed8040199
- https://www.mdpi.com/2414-6366/8/4/199/pdf?version=1680086365
- OA Status
- gold
- Cited By
- 8
- References
- 32
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4361275208
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4361275208Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3390/tropicalmed8040199Digital Object Identifier
- Title
-
Point-of-Care Diagnosis of Malaria Using a Simple, Purification-Free DNA Extraction Method Coupled with Loop-Mediated Isothermal Amplification-Lateral FlowWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-03-29Full publication date if available
- Authors
-
Meng Yee Lai, Lee Phone Youth Zen, Mohd Hafizi Abdul Hamid, Jenarun Jelip, Rose Nani Mudin, Vun Jan Shui Ivan, Lee Ngie Ping Francis, Izreena Saihidi, Yee Ling LauList of authors in order
- Landing page
-
https://doi.org/10.3390/tropicalmed8040199Publisher landing page
- PDF URL
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https://www.mdpi.com/2414-6366/8/4/199/pdf?version=1680086365Direct link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.mdpi.com/2414-6366/8/4/199/pdf?version=1680086365Direct OA link when available
- Concepts
-
Loop-mediated isothermal amplification, DNA extraction, Multiplex, Diagnosis of malaria, Malaria, DNA, Biology, Plasmodium falciparum, Chromatography, Polymerase chain reaction, Chemistry, Bioinformatics, Immunology, Genetics, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
8Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3, 2024: 4, 2023: 1Per-year citation counts (last 5 years)
- References (count)
-
32Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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