Journal of Biosciences and Medicines • Vol 04 • No 12
Point-of-Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology
January 2016 • Chunxiu Liu, Haoyuan Cai, Jian Jia, Tianyang Cao, Tong Li, Tianjun Ma, Chang Liu
We have presented a three dimensional optical protein chip that fulfills the demanding for point-of-care diagnostics in terms of ease-of-use (one step assay), miniaturization (5 μl). The artful combination of magnetic nanoparticles on chip and total internal reflection imaging (TIRI) technology permits the sensitive and rapid detection of hs-CRP (high-sensitivity C-reactive protein). The whole test was complete within 10 min using “all in one step” assay with a limit of detection of 0.1 ng/mL hs-CRP. The measuring…