Briefings in Bioinformatics • Vol 26 • No 4
Predicting protein stability changes upon mutations with dual-view ensemble learning from single sequence
June 2025 • Zhiwei Nie, Yiming Ma, Yutian Liu, Xiansong Huang, Zhihong Liu, Yang Peng, Fan Xu, Feng Yin, Zigang Li, J. Fu, Zhixiang Ren, Wenbin Zhang, Jie Chen
Abstract Predicting the protein stability changes upon mutations is one of the effective ways to improve the efficiency of protein engineering. Here, we propose a dual-view ensemble learning-based framework, DVE-stability, for mutation-induced protein stability change prediction from single sequence. DVE-stability integrates the global and local dependencies of mutations to capture the intramolecular interactions from two views through ensemble learning, in which a structural microenvironment simulation module is …