Preparation and characterization of size-controlled glioma spheroids using agarose hydrogel microwells Article Swipe
Fereshtehsadat Mirab
,
You Jung Kang
,
Sheereen Majd
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0211078
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0211078
Treatment of glioblastoma, the most common and aggressive type of primary brain tumors, is a major medical challenge and the development of new alternatives requires simple yet realistic models for these tumors. In vitro spheroid models offer attractive platforms to mimic the tumor behavior in vivo and have thus, been increasingly applied for assessment of drug efficacy in various tumors. The aim of this study was to produce and characterize size-controlled U251 glioma spheroids towards application in glioma drug evaluation studies. To this end, we fabricated agarose hydrogel microwells with cylindrical shape and diameters of 70-700 μm and applied these wells without any surface modification for glioma spheroid formation. The resultant spheroids were homogeneous in size and shape, exhibited high cell viability (> 90%), and had a similar growth rate to that of natural brain tumors. The final size of spheroids depended on cell seeding density and microwell size. The spheroids' volume increased linearly with the cell seeding density and the rate of this change increased with the well size. Lastly, we tested the therapeutic effect of an anti-cancer drug, Di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) on the resultant glioma spheroids and demonstrated the applicability of this spheroid model for drug efficacy studies.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0211078
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211078&type=printable
- OA Status
- gold
- Cited By
- 77
- References
- 35
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2911912069
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2911912069Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1371/journal.pone.0211078Digital Object Identifier
- Title
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Preparation and characterization of size-controlled glioma spheroids using agarose hydrogel microwellsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
-
2019-01-24Full publication date if available
- Authors
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Fereshtehsadat Mirab, You Jung Kang, Sheereen MajdList of authors in order
- Landing page
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https://doi.org/10.1371/journal.pone.0211078Publisher landing page
- PDF URL
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211078&type=printableDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211078&type=printableDirect OA link when available
- Concepts
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Spheroid, Glioma, Agarose, Materials science, Biomedical engineering, Chemistry, Biology, Medicine, Chromatography, In vitro, Cancer research, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
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77Total citation count in OpenAlex
- Citations by year (recent)
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2025: 4, 2024: 9, 2023: 13, 2022: 18, 2021: 15Per-year citation counts (last 5 years)
- References (count)
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35Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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