Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning Article Swipe
Jason T. Schott
,
Leslie A. Kirby
,
Peter A. Calabresi
,
Emily G. Baxi
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.3791/53764
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.3791/53764
Efficient oligodendrogenesis is the therapeutic goal of a number of areas of research including spinal cord injury, neonatal hypoxia, and demyelinating diseases such as multiple sclerosis and transverse myelitis. Myelination is required to not only facilitate rapid impulse propagation within the central nervous system, but also to provide trophic support to underlying axons. Oligodendrocyte progenitor cells (OPCs) can be studied in vitro to help identify factors that may promote or inhibit oligodendrocyte differentiation. To date, many of the methods available to evaluate this process have either required large numbers of cells, thus limiting the number of conditions that can be investigated at any one time, or labor-intensive methods of quantification. Herein, we describe a protocol for the isolation of large numbers of highly pure OPCs together with a fast and reliable method to determine oligodendrogenesis from multiple conditions simultaneously. OPCs are isolated from P5-P7 neonatal rat cortices and grown in vitro for three days prior to differentiation. Four days after differentiation, oligodendrogenesis is evaluated using a dual-infrared fluorescence-scanning assay to determine expression of the myelin protein.
Related Topics
Concepts
Multiple sclerosis
Oligodendrocyte
Neuroscience
Myelin
Myelin basic protein
Biology
Progenitor cell
Neural stem cell
Cell biology
In vitro
Progenitor
Spinal cord
Central nervous system
Stem cell
Immunology
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3791/53764
- https://www.jove.com/pdf/53764/preparation-rat-oligodendrocyte-progenitor-cultures-quantification
- OA Status
- bronze
- Cited By
- 8
- References
- 24
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2282895034
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2282895034Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3791/53764Digital Object Identifier
- Title
-
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence ScanningWork title
- Type
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articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2016Year of publication
- Publication date
-
2016-02-17Full publication date if available
- Authors
-
Jason T. Schott, Leslie A. Kirby, Peter A. Calabresi, Emily G. BaxiList of authors in order
- Landing page
-
https://doi.org/10.3791/53764Publisher landing page
- PDF URL
-
https://www.jove.com/pdf/53764/preparation-rat-oligodendrocyte-progenitor-cultures-quantificationDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
bronzeOpen access status per OpenAlex
- OA URL
-
https://www.jove.com/pdf/53764/preparation-rat-oligodendrocyte-progenitor-cultures-quantificationDirect OA link when available
- Concepts
-
Multiple sclerosis, Oligodendrocyte, Neuroscience, Myelin, Myelin basic protein, Biology, Progenitor cell, Neural stem cell, Cell biology, In vitro, Progenitor, Spinal cord, Central nervous system, Stem cell, Immunology, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
8Total citation count in OpenAlex
- Citations by year (recent)
-
2023: 1, 2020: 1, 2019: 3, 2018: 1, 2016: 2Per-year citation counts (last 5 years)
- References (count)
-
24Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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