Preserving Nature’s Treasure: A Journey into the In Vitro Conservation and Micropropagation of the Endangered Medicinal Marvel—Podophyllum hexandrum Royle Article Swipe
Zahoor Ahmad
,
Bushra Khan
,
Syed Tanveer Shah
,
Javaid Iqbal
,
Abdul Basıt
,
Muhammad Suleman Khan
,
Waleed Iqbal
,
Mohamed Farouk Elsadek
,
Aftab Jamal
,
M. Ajmal Ali
,
Domenico Prisa
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/horticulturae10080809
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/horticulturae10080809
Podophyllum hexandrum Royle, also known as Podophyllum emodi Wall, holds significant ecological, ornamental, and medicinal values. However, it has become endangered due to overexploitation, prolonged seed dormancy, slow natural regeneration, and climate change. This study developed an efficient in vitro protocol for callogenesis and micropropagation of P. hexandrum to conserve germplasm in in vitro conditions. Callus formation from various plant parts, including the leaf, stem, rhizome, radicle, and cotyledon, was induced using Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The combination of benzyladenine at 1 mg L−1 and 4-dichlorophenoxy acetic acid at 3 mg L−1 was optimal for biomass production, yielding 215.88 ± 0.31 mg, with growth per gram at 8.32 ± 0.32 and a growth rate of 13.62 ± 0.25 mg/day on MS medium. For shoot proliferation, benzyladenine (3.5 mg L−1) and naphthalene acetic acid (0.5 mg L−1) combined with activated charcoal showed the highest shoot induction percentage per explant. For shoot regeneration from calluses, 6-benzylaminopurine (0.5 mg L−1) and thidiazuron (2 mg L−1) were most effective, producing superior shoot length, number of regenerations, and regeneration percentage. Root induction was successful with α-naphthalene acetic acid supplementation (0.5 to 1.5 mg L−1) in MS medium, resulting in the highest number per explant (4.08 ± 0.08), length (5.45 ± 0.15 cm), and rooting rate (87.00 ± 1.66%) of roots in plantlets. Subculturing for callus culture was performed every 28 days for up to four subcultures to prevent nutrient depletion and toxic metabolite accumulation, ensuring tissue health and viability. Continuous subculturing of callus on MS medium maintained healthy P. hexandrum germplasm in vitro. Overall, this micropropagation protocol provides a rapid system for conserving P. hexandrum germplasm.
Related Topics
Concepts
Podophyllum
Explant culture
Shoot
Micropropagation
Biology
Callus
Murashige and Skoog medium
Botany
Rhizome
Horticulture
Chemistry
In vitro
Biochemistry
Podophyllotoxin
Stereochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/horticulturae10080809
- https://www.mdpi.com/2311-7524/10/8/809/pdf?version=1722352102
- OA Status
- gold
- Cited By
- 4
- References
- 48
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4401105169
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4401105169Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/horticulturae10080809Digital Object Identifier
- Title
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Preserving Nature’s Treasure: A Journey into the In Vitro Conservation and Micropropagation of the Endangered Medicinal Marvel—Podophyllum hexandrum RoyleWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
-
2024-07-30Full publication date if available
- Authors
-
Zahoor Ahmad, Bushra Khan, Syed Tanveer Shah, Javaid Iqbal, Abdul Basıt, Muhammad Suleman Khan, Waleed Iqbal, Mohamed Farouk Elsadek, Aftab Jamal, M. Ajmal Ali, Domenico PrisaList of authors in order
- Landing page
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https://doi.org/10.3390/horticulturae10080809Publisher landing page
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https://www.mdpi.com/2311-7524/10/8/809/pdf?version=1722352102Direct link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.mdpi.com/2311-7524/10/8/809/pdf?version=1722352102Direct OA link when available
- Concepts
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Podophyllum, Explant culture, Shoot, Micropropagation, Biology, Callus, Murashige and Skoog medium, Botany, Rhizome, Horticulture, Chemistry, In vitro, Biochemistry, Podophyllotoxin, StereochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
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4Total citation count in OpenAlex
- Citations by year (recent)
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2025: 4Per-year citation counts (last 5 years)
- References (count)
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48Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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