Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure Article Swipe

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N Buntsma , Mona Shahsavari , Aleksandra Gąsecka , Rienk Nieuwland , Ton G. van Leeuwen , Edwin van der Pol ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1016/j.rpth.2023.100171

To prevent swarm detection in a series of clinical samples, the measurement count rate of a single diluted plasma sample can be used to determine the optimal dilution factor. For our samples, flow cytometer, and settings, the optimal dilution factor is ≥1.1 × 10²-fold, while the count rate is <1.1 × 10⁴ events·s^-1.

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Dilution Chromatography Detection limit Flow cytometry Extracellular vesicles Extracellular vesicle Chemistry Analytical Chemistry (journal) Microvesicles Physics Immunology Biology Biochemistry Thermodynamics Cell biology microRNA Gene

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Type: article
Language: en
Landing Page: https://doi.org/10.1016/j.rpth.2023.100171
PDF: http://www.rpthjournal.org/article/S2475037923001449/pdf
OA Status: gold
Cited By: 12
References: 24
Related Works: 10
OpenAlex ID: https://openalex.org/W4367692428

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DOI: https://doi.org/10.1016/j.rpth.2023.100171

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Title: Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure

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Language: en

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Publication year: 2023

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Publication date: 2023-05-01

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Authors: N Buntsma, Mona Shahsavari, Aleksandra Gąsecka, Rienk Nieuwland, Ton G. van Leeuwen, Edwin van der Pol

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Landing page: https://doi.org/10.1016/j.rpth.2023.100171

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Concepts: Dilution, Chromatography, Detection limit, Flow cytometry, Extracellular vesicles, Extracellular vesicle, Chemistry, Analytical Chemistry (journal), Microvesicles, Physics, Immunology, Biology, Biochemistry, Thermodynamics, Cell biology, microRNA, Gene

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Cited by: 12

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