Production of 1,4-butanediol through Clostridia C4 pathways Article Swipe
Mingwei Zha
,
Jiangxin Gu
,
Jian Chen
,
Huifang Zhang
,
Mengting Li
,
Yong Chen
,
Huanqing Niu
,
Chenjie Zhu
,
Ting Guo
,
Zhenyu Wang
,
Dong Liu
,
Hanjie Ying
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-4392475/v1
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-4392475/v1
1,4-butanediol (1,4-BDO) is an important building block in the chemical industry that has been mainly produced from fossil fuels, but now biosynthesis of 1,4-BDO has received more and more attention due to environmental issues. The Clostridia C4 pathway produces an intermediate crotonyl-CoA which could be diverted to 1,4-BDO by 4-hydroxybutyryl-CoA dehydratase (4HBD). Here, we compared this pathway with other 1,4-BDO biosynthesis pathways and illustrated its potential advantages regarding cellular energy conservation and theoretical yield. Then, the feasibility of 1,4-BDO production in this way was tested by simply introducing a single 4HBD in Clostridium acetobutylicum that natively produced the C4 intermediate and a variety of aldehyde/alcohol dehydrogenases (AdhE). Five different 4HBD genes were screened and the Cbei-2100 gene from Clostridium beijerinckii was the most effective, producing 66 mg/L of 1,4-BDO. To block the metabolic flux towards the main product butanol, disruption of butyryl-CoA dehydrogenase (Bcd) was tried but failed, while inactivation of its homologue (FAD/FMN-containing dehydrogenase, Fcd) obtained little effect. Alternatively, the electron-transferring flavoprotein EtfA coupled with Bcd was inactivated, and 1,4-BDO production was greatly increased to 182 mg/L. In conclusion, this study demonstrated the feasibility of 1,4-BDO production through the Clostridia C4 pathway. Further blocking of the competing flux towards butanol would be effective to improve the production in the future.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-4392475/v1
- https://www.researchsquare.com/article/rs-4392475/latest.pdf
- OA Status
- green
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4398140425
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4398140425Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-4392475/v1Digital Object Identifier
- Title
-
Production of 1,4-butanediol through Clostridia C4 pathwaysWork title
- Type
-
preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-05-20Full publication date if available
- Authors
-
Mingwei Zha, Jiangxin Gu, Jian Chen, Huifang Zhang, Mengting Li, Yong Chen, Huanqing Niu, Chenjie Zhu, Ting Guo, Zhenyu Wang, Dong Liu, Hanjie YingList of authors in order
- Landing page
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https://doi.org/10.21203/rs.3.rs-4392475/v1Publisher landing page
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https://www.researchsquare.com/article/rs-4392475/latest.pdfDirect link to full text PDF
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
- OA URL
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https://www.researchsquare.com/article/rs-4392475/latest.pdfDirect OA link when available
- Concepts
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Clostridia, 2,3-Butanediol, 1,4-Butanediol, Production (economics), Computational biology, Chemistry, Biology, Food science, Biochemistry, Genetics, Economics, Bacteria, Fermentation, Catalysis, MacroeconomicsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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