Production of 1,4-butanediol through Clostridia C4 pathways Article Swipe
Mingwei Zha
,
Jiangxin Gu
,
Jian Chen
,
Huifang Zhang
,
Mengting Li
,
Yong Chen
,
Huanqing Niu
,
Chenjie Zhu
,
Ting Guo
,
Zhenyu Wang
,
Dong Liu
,
Hanjie Ying
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1080/13102818.2024.2415039
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1080/13102818.2024.2415039
1,4-butanediol (1,4-BDO) is an important building block in the chemical industry that has been mainly produced from fossil fuels, but now biosynthesis of 1,4-BDO has received more and more attention due to environmental issues. The Clostridia C4 pathway produces an intermediate crotonyl-CoA which could be diverted to 1,4-BDO by 4-hydroxybutyryl-CoA dehydratase (4HBD). Here, we compared this pathway with other 1,4-BDO biosynthesis pathways and illustrated its potential advantages regarding cellular energy conservation and theoretical yield. Then, the feasibility of 1,4-BDO production in this way was tested by introducing a single 4HBD in Clostridium acetobutylicum that natively produced the C4 intermediate and a variety of aldehyde/alcohol dehydrogenases (AdhE). Five different 4HBD genes were screened and the Cbei-2100 gene from Clostridium beijerinckii was the most effective, producing 0.066 mg/mL of 1,4-BDO. To block the metabolic flux towards the main product butanol, disruption of butyryl-CoA dehydrogenase (Bcd) was tried but failed, while inactivation of its homologue (FAD/FMN-containing dehydrogenase, Fcd) obtained little effect. Alternatively, the electron-transferring flavoprotein EtfA coupled with Bcd was inactivated, and 1,4-BDO production was greatly increased to 0.182 mg/mL. In conclusion, this study demonstrated the feasibility of 1,4-BDO production through the Clostridia C4 pathway. Further blocking of the competing flux towards butanol would be effective to improve the production of in the future.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1080/13102818.2024.2415039
- OA Status
- gold
- References
- 23
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4403359953
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4403359953Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1080/13102818.2024.2415039Digital Object Identifier
- Title
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Production of 1,4-butanediol through Clostridia C4 pathwaysWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-10-12Full publication date if available
- Authors
-
Mingwei Zha, Jiangxin Gu, Jian Chen, Huifang Zhang, Mengting Li, Yong Chen, Huanqing Niu, Chenjie Zhu, Ting Guo, Zhenyu Wang, Dong Liu, Hanjie YingList of authors in order
- Landing page
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https://doi.org/10.1080/13102818.2024.2415039Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1080/13102818.2024.2415039Direct OA link when available
- Concepts
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Clostridia, 2,3-Butanediol, Food science, Production (economics), Biology, Chemistry, Biochemistry, Metabolic pathway, Biotechnology, Computational biology, Fermentation, Enzyme, Bacteria, Genetics, Economics, MacroeconomicsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
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23Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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