Proteome analysis of the Gram-positive fish pathogen Renibacterium salmoninarum reveals putative role of membrane vesicles in virulence Article Swipe
Tobias Kroniger
,
Daniel Flender
,
Rabea Schlüter
,
Bernd Köllner
,
Anke Trautwein‐Schult
,
Dörte Becher
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-022-06130-w
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1038/s41598-022-06130-w
Bacterial kidney disease (BKD) is a chronic bacterial disease affecting both wild and farmed salmonids. The causative agent for BKD is the Gram-positive fish pathogen Renibacterium salmoninarum . As treatment and prevention of BKD have proven to be difficult, it is important to know and identify the key bacterial proteins that interact with the host. We used subcellular fractionation to report semi-quantitative data for the cytosolic, membrane, extracellular, and membrane vesicle (MV) proteome of R. salmoninarum . These data can aid as a backbone for more targeted experiments regarding the development of new drugs for the treatment of BKD. Further analysis was focused on the MV proteome, where both major immunosuppressive proteins P57/Msa and P22 and proteins involved in bacterial adhesion were found in high abundance. Interestingly, the P22 protein was relatively enriched only in the extracellular and MV fraction, implicating that MVs may play a role in host–pathogen interaction. Compared to the other subcellular fractions, the MVs were also relatively enriched in lipoproteins and all four cell wall hydrolases belonging to the New Lipoprotein C/Protein of 60 kDa (NlpC/P60) family were detected, suggesting an involvement in the formation of the MVs.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41598-022-06130-w
- https://www.nature.com/articles/s41598-022-06130-w.pdf
- OA Status
- gold
- Cited By
- 8
- References
- 63
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4212776248
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4212776248Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1038/s41598-022-06130-wDigital Object Identifier
- Title
-
Proteome analysis of the Gram-positive fish pathogen Renibacterium salmoninarum reveals putative role of membrane vesicles in virulenceWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-02-22Full publication date if available
- Authors
-
Tobias Kroniger, Daniel Flender, Rabea Schlüter, Bernd Köllner, Anke Trautwein‐Schult, Dörte BecherList of authors in order
- Landing page
-
https://doi.org/10.1038/s41598-022-06130-wPublisher landing page
- PDF URL
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https://www.nature.com/articles/s41598-022-06130-w.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
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-
goldOpen access status per OpenAlex
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https://www.nature.com/articles/s41598-022-06130-w.pdfDirect OA link when available
- Concepts
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Virulence, Pathogen, Proteome, Microbiology, Biology, Gram, Fish
, Bacteria, Bioinformatics, Gene, Biochemistry, Fishery, Genetics Top concepts (fields/topics) attached by OpenAlex - Cited by
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8Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1, 2024: 2, 2023: 1, 2022: 4Per-year citation counts (last 5 years)
- References (count)
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63Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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