Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation Article Swipe
Rachel E. Heap
,
José Luis Marín‐Rubio
,
Julien Peltier
,
Tiaan Heunis
,
Abeer Dannoura
,
Adam J. Moore
,
Matthias Trost
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.08.20.259515
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.08.20.259515
Bone marrow-derived macrophages (BMDMs) are a key model system to study macrophage biology in vitro . Commonly used methods to differentiate macrophages from bone marrow are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 cell conditioned media (LCCM) and how it affects BMDM phenotype. Here, we used quantitative mass spectrometry to characterise the kinetics of protein secretion from L929 cells over a two-week period, identifying 2,193 proteins. While M-CSF is very abundant in LCCM, we identified several other immune-regulatory proteins such as macrophage migration inhibitory factor (MIF), osteopontin and chemokines such as Ccl2 and Ccl7 at surprisingly high abundance levels. We therefore further characterised the proteomes of BMDMs after differentiation with M-CSF, M-CSF + MIF or LCCM, respectively. Interestingly, macrophages differentiated with LCCM induced a stronger anti-inflammatory M1 phenotype that those differentiated with M-CSF. This resource will be valuable to all researchers using LCCM for the differentiation of BMDMs.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2020.08.20.259515
- https://www.biorxiv.org/content/biorxiv/early/2020/11/14/2020.08.20.259515.full.pdf
- OA Status
- green
- Cited By
- 3
- References
- 38
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3061073885
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3061073885Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2020.08.20.259515Digital Object Identifier
- Title
-
Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiationWork title
- Type
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preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-08-20Full publication date if available
- Authors
-
Rachel E. Heap, José Luis Marín‐Rubio, Julien Peltier, Tiaan Heunis, Abeer Dannoura, Adam J. Moore, Matthias TrostList of authors in order
- Landing page
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https://doi.org/10.1101/2020.08.20.259515Publisher landing page
- PDF URL
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https://www.biorxiv.org/content/biorxiv/early/2020/11/14/2020.08.20.259515.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2020/11/14/2020.08.20.259515.full.pdfDirect OA link when available
- Concepts
-
Osteopontin, Secretion, Proteome, Chemokine, Phenotype, Bone marrow, Cell biology, Macrophage, Cellular differentiation, Macrophage migration inhibitory factor, CCL2, Biology, Cytokine, In vitro, Inflammation, Biochemistry, Immunology, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
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3Total citation count in OpenAlex
- Citations by year (recent)
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2024: 1, 2021: 2Per-year citation counts (last 5 years)
- References (count)
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38Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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