Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis Article Swipe
Thalia Pacheco‐Fernández
,
Laura Klenow
,
Nazli Azodi
,
Hannah L. Markle
,
Matthew C. Bernier
,
Greta Volpedo
,
Timur Oljuskin
,
Parna Bhattacharya
,
Shinjiro Hamano
,
Greg Matlashewski
,
Hira L. Nakhasi
,
Abhay R. Satoskar
,
Sreenivas Gannavaram
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2025.103817
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2025.103817
Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic pathways and altered metabolites. We then detail procedures for verifying the metabolite role in immune response via PCR and ELISA. This protocol focuses on cutaneous leishmaniasis models, but the analyses here are applicable to other infection models. For complete details on the use and execution of this protocol, please refer to Volpedo et al.1 and Oljuskin et al.2.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.xpro.2025.103817
- OA Status
- gold
- References
- 10
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4412474129
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4412474129Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.xpro.2025.103817Digital Object Identifier
- Title
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Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysisWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-07-16Full publication date if available
- Authors
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Thalia Pacheco‐Fernández, Laura Klenow, Nazli Azodi, Hannah L. Markle, Matthew C. Bernier, Greta Volpedo, Timur Oljuskin, Parna Bhattacharya, Shinjiro Hamano, Greg Matlashewski, Hira L. Nakhasi, Abhay R. Satoskar, Sreenivas GannavaramList of authors in order
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https://doi.org/10.1016/j.xpro.2025.103817Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://doi.org/10.1016/j.xpro.2025.103817Direct OA link when available
- Concepts
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Metabolomics, Leishmania, Immune system, Immunology, Biology, Leishmania major, Computational biology, Medicine, Bioinformatics, Parasite hosting, Computer science, World Wide WebTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- References (count)
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10Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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