Protocol for production of homogeneous iPSC spheroids and microtissues using the suction technique Article Swipe
Taijun Moriwaki
,
Hidenori Tani
,
Kotaro Haga
,
Shugo Tohyama
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2025.103891
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2025.103891
Three-dimensional cultures mimic in vivo environments better than two-dimensional cultures and are often used in drug discovery research. Herein, we present a protocol for producing homogeneous induced pluripotent stem cell (iPSC) spheroids and microtissues using the suction technique. We describe steps for preparing the suction device, preparing and seeding cells, and suction sedimentation of cells. We then detail procedures for self-assembly and evaluation of spheroids. For complete details on the use and execution of this protocol, please refer to Moriwaki et al.1.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.xpro.2025.103891
- OA Status
- gold
- Cited By
- 1
- References
- 5
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4411231591
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4411231591Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1016/j.xpro.2025.103891Digital Object Identifier
- Title
-
Protocol for production of homogeneous iPSC spheroids and microtissues using the suction techniqueWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-06-01Full publication date if available
- Authors
-
Taijun Moriwaki, Hidenori Tani, Kotaro Haga, Shugo TohyamaList of authors in order
- Landing page
-
https://doi.org/10.1016/j.xpro.2025.103891Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1016/j.xpro.2025.103891Direct OA link when available
- Concepts
-
Spheroid, Homogeneous, Suction, Protocol (science), Computer science, Materials science, Biomedical engineering, Chemistry, Engineering, Medicine, Physics, Mechanical engineering, Pathology, Statistical physics, In vitro, Biochemistry, Alternative medicineTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1Per-year citation counts (last 5 years)
- References (count)
-
5Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4411231591 |
|---|---|
| doi | https://doi.org/10.1016/j.xpro.2025.103891 |
| ids.doi | https://doi.org/10.1016/j.xpro.2025.103891 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/40516047 |
| ids.openalex | https://openalex.org/W4411231591 |
| fwci | 2.17737573 |
| mesh[0].qualifier_ui | Q000166 |
| mesh[0].descriptor_ui | D057026 |
| mesh[0].is_major_topic | True |
| mesh[0].qualifier_name | cytology |
| mesh[0].descriptor_name | Induced Pluripotent Stem Cells |
| mesh[1].qualifier_ui | Q000166 |
| mesh[1].descriptor_ui | D018874 |
| mesh[1].is_major_topic | True |
| mesh[1].qualifier_name | cytology |
| mesh[1].descriptor_name | Spheroids, Cellular |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D006801 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Humans |
| mesh[3].qualifier_ui | Q000379 |
| mesh[3].descriptor_ui | D018929 |
| mesh[3].is_major_topic | True |
| mesh[3].qualifier_name | methods |
| mesh[3].descriptor_name | Cell Culture Techniques |
| mesh[4].qualifier_ui | Q000379 |
| mesh[4].descriptor_ui | D013396 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | methods |
| mesh[4].descriptor_name | Suction |
| mesh[5].qualifier_ui | Q000379 |
| mesh[5].descriptor_ui | D000087603 |
| mesh[5].is_major_topic | True |
| mesh[5].qualifier_name | methods |
| mesh[5].descriptor_name | Cell Culture Techniques, Three Dimensional |
| mesh[6].qualifier_ui | Q000166 |
| mesh[6].descriptor_ui | D057026 |
| mesh[6].is_major_topic | True |
| mesh[6].qualifier_name | cytology |
| mesh[6].descriptor_name | Induced Pluripotent Stem Cells |
| mesh[7].qualifier_ui | Q000166 |
| mesh[7].descriptor_ui | D018874 |
| mesh[7].is_major_topic | True |
| mesh[7].qualifier_name | cytology |
| mesh[7].descriptor_name | Spheroids, Cellular |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D006801 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Humans |
| mesh[9].qualifier_ui | Q000379 |
| mesh[9].descriptor_ui | D018929 |
| mesh[9].is_major_topic | True |
| mesh[9].qualifier_name | methods |
| mesh[9].descriptor_name | Cell Culture Techniques |
| mesh[10].qualifier_ui | Q000379 |
| mesh[10].descriptor_ui | D013396 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | methods |
| mesh[10].descriptor_name | Suction |
| mesh[11].qualifier_ui | Q000379 |
| mesh[11].descriptor_ui | D000087603 |
| mesh[11].is_major_topic | True |
| mesh[11].qualifier_name | methods |
| mesh[11].descriptor_name | Cell Culture Techniques, Three Dimensional |
| type | article |
| title | Protocol for production of homogeneous iPSC spheroids and microtissues using the suction technique |
| awards[0].id | https://openalex.org/G8166103657 |
| awards[0].funder_id | https://openalex.org/F4320334764 |
| awards[0].display_name | |
| awards[0].funder_award_id | 23K27683 |
| awards[0].funder_display_name | Japan Society for the Promotion of Science |
| awards[1].id | https://openalex.org/G6652211686 |
| awards[1].funder_id | https://openalex.org/F4320311405 |
| awards[1].display_name | |
| awards[1].funder_award_id | 24bm1123010 |
| awards[1].funder_display_name | Japan Agency for Medical Research and Development |
| awards[2].id | https://openalex.org/G6253175424 |
| awards[2].funder_id | https://openalex.org/F4320311405 |
| awards[2].display_name | |
| awards[2].funder_award_id | 25bm1223022 |
| awards[2].funder_display_name | Japan Agency for Medical Research and Development |
| biblio.issue | 2 |
| biblio.volume | 6 |
| biblio.last_page | 103891 |
| biblio.first_page | 103891 |
| topics[0].id | https://openalex.org/T10783 |
| topics[0].field.id | https://openalex.org/fields/22 |
| topics[0].field.display_name | Engineering |
| topics[0].score | 0.9983000159263611 |
| topics[0].domain.id | https://openalex.org/domains/3 |
| topics[0].domain.display_name | Physical Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2203 |
| topics[0].subfield.display_name | Automotive Engineering |
| topics[0].display_name | Additive Manufacturing and 3D Printing Technologies |
| topics[1].id | https://openalex.org/T10505 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9879999756813049 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1312 |
| topics[1].subfield.display_name | Molecular Biology |
| topics[1].display_name | Pluripotent Stem Cells Research |
| topics[2].id | https://openalex.org/T11190 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.9771999716758728 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2204 |
| topics[2].subfield.display_name | Biomedical Engineering |
| topics[2].display_name | 3D Printing in Biomedical Research |
| funders[0].id | https://openalex.org/F4320311405 |
| funders[0].ror | https://ror.org/004rtk039 |
| funders[0].display_name | Japan Agency for Medical Research and Development |
| funders[1].id | https://openalex.org/F4320318204 |
| funders[1].ror | |
| funders[1].display_name | Kanagawa Institute of Industrial Science and Technology |
| funders[2].id | https://openalex.org/F4320334764 |
| funders[2].ror | https://ror.org/00hhkn466 |
| funders[2].display_name | Japan Society for the Promotion of Science |
| is_xpac | False |
| apc_list.value | 168540 |
| apc_list.currency | JPY |
| apc_list.value_usd | 1500 |
| apc_paid.value | 168540 |
| apc_paid.currency | JPY |
| apc_paid.value_usd | 1500 |
| concepts[0].id | https://openalex.org/C175369904 |
| concepts[0].level | 3 |
| concepts[0].score | 0.8666480779647827 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q208395 |
| concepts[0].display_name | Spheroid |
| concepts[1].id | https://openalex.org/C66882249 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6738786697387695 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q169336 |
| concepts[1].display_name | Homogeneous |
| concepts[2].id | https://openalex.org/C23795335 |
| concepts[2].level | 2 |
| concepts[2].score | 0.6053634881973267 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q1790295 |
| concepts[2].display_name | Suction |
| concepts[3].id | https://openalex.org/C2780385302 |
| concepts[3].level | 3 |
| concepts[3].score | 0.4812621772289276 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q367158 |
| concepts[3].display_name | Protocol (science) |
| concepts[4].id | https://openalex.org/C41008148 |
| concepts[4].level | 0 |
| concepts[4].score | 0.3619420528411865 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q21198 |
| concepts[4].display_name | Computer science |
| concepts[5].id | https://openalex.org/C192562407 |
| concepts[5].level | 0 |
| concepts[5].score | 0.35927635431289673 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q228736 |
| concepts[5].display_name | Materials science |
| concepts[6].id | https://openalex.org/C136229726 |
| concepts[6].level | 1 |
| concepts[6].score | 0.3293761610984802 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q327092 |
| concepts[6].display_name | Biomedical engineering |
| concepts[7].id | https://openalex.org/C185592680 |
| concepts[7].level | 0 |
| concepts[7].score | 0.28085535764694214 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[7].display_name | Chemistry |
| concepts[8].id | https://openalex.org/C127413603 |
| concepts[8].level | 0 |
| concepts[8].score | 0.1770685613155365 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q11023 |
| concepts[8].display_name | Engineering |
| concepts[9].id | https://openalex.org/C71924100 |
| concepts[9].level | 0 |
| concepts[9].score | 0.1637270450592041 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q11190 |
| concepts[9].display_name | Medicine |
| concepts[10].id | https://openalex.org/C121332964 |
| concepts[10].level | 0 |
| concepts[10].score | 0.1618744134902954 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q413 |
| concepts[10].display_name | Physics |
| concepts[11].id | https://openalex.org/C78519656 |
| concepts[11].level | 1 |
| concepts[11].score | 0.11583182215690613 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q101333 |
| concepts[11].display_name | Mechanical engineering |
| concepts[12].id | https://openalex.org/C142724271 |
| concepts[12].level | 1 |
| concepts[12].score | 0.11551204323768616 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7208 |
| concepts[12].display_name | Pathology |
| concepts[13].id | https://openalex.org/C121864883 |
| concepts[13].level | 1 |
| concepts[13].score | 0.06042739748954773 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q677916 |
| concepts[13].display_name | Statistical physics |
| concepts[14].id | https://openalex.org/C202751555 |
| concepts[14].level | 2 |
| concepts[14].score | 0.05809742212295532 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q221681 |
| concepts[14].display_name | In vitro |
| concepts[15].id | https://openalex.org/C55493867 |
| concepts[15].level | 1 |
| concepts[15].score | 0.0 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[15].display_name | Biochemistry |
| concepts[16].id | https://openalex.org/C204787440 |
| concepts[16].level | 2 |
| concepts[16].score | 0.0 |
| concepts[16].wikidata | https://www.wikidata.org/wiki/Q188504 |
| concepts[16].display_name | Alternative medicine |
| keywords[0].id | https://openalex.org/keywords/spheroid |
| keywords[0].score | 0.8666480779647827 |
| keywords[0].display_name | Spheroid |
| keywords[1].id | https://openalex.org/keywords/homogeneous |
| keywords[1].score | 0.6738786697387695 |
| keywords[1].display_name | Homogeneous |
| keywords[2].id | https://openalex.org/keywords/suction |
| keywords[2].score | 0.6053634881973267 |
| keywords[2].display_name | Suction |
| keywords[3].id | https://openalex.org/keywords/protocol |
| keywords[3].score | 0.4812621772289276 |
| keywords[3].display_name | Protocol (science) |
| keywords[4].id | https://openalex.org/keywords/computer-science |
| keywords[4].score | 0.3619420528411865 |
| keywords[4].display_name | Computer science |
| keywords[5].id | https://openalex.org/keywords/materials-science |
| keywords[5].score | 0.35927635431289673 |
| keywords[5].display_name | Materials science |
| keywords[6].id | https://openalex.org/keywords/biomedical-engineering |
| keywords[6].score | 0.3293761610984802 |
| keywords[6].display_name | Biomedical engineering |
| keywords[7].id | https://openalex.org/keywords/chemistry |
| keywords[7].score | 0.28085535764694214 |
| keywords[7].display_name | Chemistry |
| keywords[8].id | https://openalex.org/keywords/engineering |
| keywords[8].score | 0.1770685613155365 |
| keywords[8].display_name | Engineering |
| keywords[9].id | https://openalex.org/keywords/medicine |
| keywords[9].score | 0.1637270450592041 |
| keywords[9].display_name | Medicine |
| keywords[10].id | https://openalex.org/keywords/physics |
| keywords[10].score | 0.1618744134902954 |
| keywords[10].display_name | Physics |
| keywords[11].id | https://openalex.org/keywords/mechanical-engineering |
| keywords[11].score | 0.11583182215690613 |
| keywords[11].display_name | Mechanical engineering |
| keywords[12].id | https://openalex.org/keywords/pathology |
| keywords[12].score | 0.11551204323768616 |
| keywords[12].display_name | Pathology |
| keywords[13].id | https://openalex.org/keywords/statistical-physics |
| keywords[13].score | 0.06042739748954773 |
| keywords[13].display_name | Statistical physics |
| keywords[14].id | https://openalex.org/keywords/in-vitro |
| keywords[14].score | 0.05809742212295532 |
| keywords[14].display_name | In vitro |
| language | en |
| locations[0].id | doi:10.1016/j.xpro.2025.103891 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4210212477 |
| locations[0].source.issn | 2666-1667 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2666-1667 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | STAR Protocols |
| locations[0].source.host_organization | https://openalex.org/P4310320990 |
| locations[0].source.host_organization_name | Elsevier BV |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320990 |
| locations[0].source.host_organization_lineage_names | Elsevier BV |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | STAR Protocols |
| locations[0].landing_page_url | https://doi.org/10.1016/j.xpro.2025.103891 |
| locations[1].id | pmid:40516047 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | STAR protocols |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/40516047 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:12207686 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | other-oa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/other-oa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | STAR Protoc |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/12207686 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5057785221 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-0432-3363 |
| authorships[0].author.display_name | Taijun Moriwaki |
| authorships[0].countries | JP |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I145673806 |
| authorships[0].affiliations[0].raw_affiliation_string | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan. Electronic address: [email protected]. |
| authorships[0].institutions[0].id | https://openalex.org/I145673806 |
| authorships[0].institutions[0].ror | https://ror.org/046f6cx68 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I145673806 |
| authorships[0].institutions[0].country_code | JP |
| authorships[0].institutions[0].display_name | Fujita Health University |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Taijun Moriwaki |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan. Electronic address: [email protected]. |
| authorships[1].author.id | https://openalex.org/A5103095197 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-5042-6187 |
| authorships[1].author.display_name | Hidenori Tani |
| authorships[1].countries | JP |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I145673806, https://openalex.org/I203951103 |
| authorships[1].affiliations[0].raw_affiliation_string | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. |
| authorships[1].institutions[0].id | https://openalex.org/I145673806 |
| authorships[1].institutions[0].ror | https://ror.org/046f6cx68 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I145673806 |
| authorships[1].institutions[0].country_code | JP |
| authorships[1].institutions[0].display_name | Fujita Health University |
| authorships[1].institutions[1].id | https://openalex.org/I203951103 |
| authorships[1].institutions[1].ror | https://ror.org/02kn6nx58 |
| authorships[1].institutions[1].type | education |
| authorships[1].institutions[1].lineage | https://openalex.org/I203951103 |
| authorships[1].institutions[1].country_code | JP |
| authorships[1].institutions[1].display_name | Keio University |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Hidenori Tani |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. |
| authorships[2].author.id | https://openalex.org/A5060883649 |
| authorships[2].author.orcid | https://orcid.org/0000-0003-2697-2097 |
| authorships[2].author.display_name | Kotaro Haga |
| authorships[2].countries | JP |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I145673806 |
| authorships[2].affiliations[0].raw_affiliation_string | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan. |
| authorships[2].institutions[0].id | https://openalex.org/I145673806 |
| authorships[2].institutions[0].ror | https://ror.org/046f6cx68 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I145673806 |
| authorships[2].institutions[0].country_code | JP |
| authorships[2].institutions[0].display_name | Fujita Health University |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Kotaro Haga |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan. |
| authorships[3].author.id | https://openalex.org/A5082913618 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-5898-3543 |
| authorships[3].author.display_name | Shugo Tohyama |
| authorships[3].countries | JP |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I145673806, https://openalex.org/I203951103 |
| authorships[3].affiliations[0].raw_affiliation_string | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan; Keio University Regenerative Medicine Research Center, Kawasaki, Kanagawa, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. Electronic address: [email protected]. |
| authorships[3].institutions[0].id | https://openalex.org/I145673806 |
| authorships[3].institutions[0].ror | https://ror.org/046f6cx68 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I145673806 |
| authorships[3].institutions[0].country_code | JP |
| authorships[3].institutions[0].display_name | Fujita Health University |
| authorships[3].institutions[1].id | https://openalex.org/I203951103 |
| authorships[3].institutions[1].ror | https://ror.org/02kn6nx58 |
| authorships[3].institutions[1].type | education |
| authorships[3].institutions[1].lineage | https://openalex.org/I203951103 |
| authorships[3].institutions[1].country_code | JP |
| authorships[3].institutions[1].display_name | Keio University |
| authorships[3].author_position | last |
| authorships[3].raw_author_name | Shugo Tohyama |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Fujita Medical Innovation Center Tokyo, Fujita Health University, Ohta, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kawasaki, Kanagawa, Japan; Keio University Regenerative Medicine Research Center, Kawasaki, Kanagawa, Japan; Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan. Electronic address: [email protected]. |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.1016/j.xpro.2025.103891 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Protocol for production of homogeneous iPSC spheroids and microtissues using the suction technique |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10783 |
| primary_topic.field.id | https://openalex.org/fields/22 |
| primary_topic.field.display_name | Engineering |
| primary_topic.score | 0.9983000159263611 |
| primary_topic.domain.id | https://openalex.org/domains/3 |
| primary_topic.domain.display_name | Physical Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2203 |
| primary_topic.subfield.display_name | Automotive Engineering |
| primary_topic.display_name | Additive Manufacturing and 3D Printing Technologies |
| related_works | https://openalex.org/W2050956753, https://openalex.org/W2184533732, https://openalex.org/W2034330200, https://openalex.org/W3045873720, https://openalex.org/W2906127908, https://openalex.org/W2111152401, https://openalex.org/W2408054456, https://openalex.org/W3046890897, https://openalex.org/W4207051211, https://openalex.org/W2906198848 |
| cited_by_count | 1 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1016/j.xpro.2025.103891 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4210212477 |
| best_oa_location.source.issn | 2666-1667 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2666-1667 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | STAR Protocols |
| best_oa_location.source.host_organization | https://openalex.org/P4310320990 |
| best_oa_location.source.host_organization_name | Elsevier BV |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320990 |
| best_oa_location.source.host_organization_lineage_names | Elsevier BV |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | STAR Protocols |
| best_oa_location.landing_page_url | https://doi.org/10.1016/j.xpro.2025.103891 |
| primary_location.id | doi:10.1016/j.xpro.2025.103891 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4210212477 |
| primary_location.source.issn | 2666-1667 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2666-1667 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | STAR Protocols |
| primary_location.source.host_organization | https://openalex.org/P4310320990 |
| primary_location.source.host_organization_name | Elsevier BV |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320990 |
| primary_location.source.host_organization_lineage_names | Elsevier BV |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | STAR Protocols |
| primary_location.landing_page_url | https://doi.org/10.1016/j.xpro.2025.103891 |
| publication_date | 2025-06-01 |
| publication_year | 2025 |
| referenced_works | https://openalex.org/W6859552426, https://openalex.org/W2959455916, https://openalex.org/W2007467041, https://openalex.org/W2138977668, https://openalex.org/W4389891844 |
| referenced_works_count | 5 |
| abstract_inverted_index.a | 21 |
| abstract_inverted_index.We | 38, 55 |
| abstract_inverted_index.et | 80 |
| abstract_inverted_index.in | 3, 14 |
| abstract_inverted_index.of | 53, 63, 73 |
| abstract_inverted_index.on | 68 |
| abstract_inverted_index.to | 78 |
| abstract_inverted_index.we | 19 |
| abstract_inverted_index.For | 65 |
| abstract_inverted_index.and | 10, 32, 47, 50, 61, 71 |
| abstract_inverted_index.are | 11 |
| abstract_inverted_index.for | 23, 41, 59 |
| abstract_inverted_index.the | 35, 43, 69 |
| abstract_inverted_index.use | 70 |
| abstract_inverted_index.cell | 29 |
| abstract_inverted_index.drug | 15 |
| abstract_inverted_index.stem | 28 |
| abstract_inverted_index.than | 7 |
| abstract_inverted_index.then | 56 |
| abstract_inverted_index.this | 74 |
| abstract_inverted_index.used | 13 |
| abstract_inverted_index.vivo | 4 |
| abstract_inverted_index.mimic | 2 |
| abstract_inverted_index.often | 12 |
| abstract_inverted_index.refer | 77 |
| abstract_inverted_index.steps | 40 |
| abstract_inverted_index.using | 34 |
| abstract_inverted_index.(iPSC) | 30 |
| abstract_inverted_index.better | 6 |
| abstract_inverted_index.cells, | 49 |
| abstract_inverted_index.cells. | 54 |
| abstract_inverted_index.detail | 57 |
| abstract_inverted_index.please | 76 |
| abstract_inverted_index.Herein, | 18 |
| abstract_inverted_index.details | 67 |
| abstract_inverted_index.device, | 45 |
| abstract_inverted_index.induced | 26 |
| abstract_inverted_index.present | 20 |
| abstract_inverted_index.seeding | 48 |
| abstract_inverted_index.suction | 36, 44, 51 |
| abstract_inverted_index.Moriwaki | 79 |
| abstract_inverted_index.complete | 66 |
| abstract_inverted_index.cultures | 1, 9 |
| abstract_inverted_index.describe | 39 |
| abstract_inverted_index.protocol | 22 |
| abstract_inverted_index.discovery | 16 |
| abstract_inverted_index.execution | 72 |
| abstract_inverted_index.preparing | 42, 46 |
| abstract_inverted_index.producing | 24 |
| abstract_inverted_index.protocol, | 75 |
| abstract_inverted_index.research. | 17 |
| abstract_inverted_index.spheroids | 31 |
| abstract_inverted_index.evaluation | 62 |
| abstract_inverted_index.procedures | 58 |
| abstract_inverted_index.spheroids. | 64 |
| abstract_inverted_index.technique. | 37 |
| abstract_inverted_index.homogeneous | 25 |
| abstract_inverted_index.pluripotent | 27 |
| abstract_inverted_index.environments | 5 |
| abstract_inverted_index.microtissues | 33 |
| abstract_inverted_index.sedimentation | 52 |
| abstract_inverted_index.self-assembly | 60 |
| abstract_inverted_index.two-dimensional | 8 |
| abstract_inverted_index.al.<sup>1</sup>. | 81 |
| abstract_inverted_index.Three-dimensional | 0 |
| cited_by_percentile_year.max | 95 |
| cited_by_percentile_year.min | 91 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| citation_normalized_percentile.value | 0.78973228 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |