Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains Article Swipe

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Hannah Tabakh , Benjamin W. Jester , Hui Zhao , Rolf Kuestner , Nhi Khuong , Chelsea Shanitta , Ryo Takeuchi , James Roberts ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1016/j.xpro.2023.102087

Here, we present a protocol for harnessing the natural transformability of the edible algae Arthrospira platensis (common name: spirulina) to generate strains that express heterologous proteins. We describe the preparation of plasmids and the steps to grow A. platensis. We then detail the transformation and passage of the strains, followed by genomic DNA extraction and genotyping to assess integration of the gene of interest. This simple transformation protocol can be applied to genome manipulation of edible algae. For complete details on the use and execution of this protocol, please refer to Jester et al. (2022).¹.

Related Topics

Spirulina (Dietary Supplement)

Biology

Algae

Computational Biology

Concepts

Spirulina (dietary supplement) Transformation (genetics) Heterologous Plasmid Arthrospira Biology Genome Algae Gene Heterologous expression genomic DNA Computational biology Genetics Botany Cyanobacteria Recombinant DNA Ecology Bacteria Raw material

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Type: article
Language: en
Landing Page: https://doi.org/10.1016/j.xpro.2023.102087
OA Status: gold
Cited By: 6
References: 6
Related Works: 10
OpenAlex ID: https://openalex.org/W4319456080

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DOI: https://doi.org/10.1016/j.xpro.2023.102087

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Title: Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains

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Type: article

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Language: en

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Publication year: 2023

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Publication date: 2023-02-08

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Authors: Hannah Tabakh, Benjamin W. Jester, Hui Zhao, Rolf Kuestner, Nhi Khuong, Chelsea Shanitta, Ryo Takeuchi, James Roberts

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Landing page: https://doi.org/10.1016/j.xpro.2023.102087

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Concepts: Spirulina (dietary supplement), Transformation (genetics), Heterologous, Plasmid, Arthrospira, Biology, Genome, Algae, Gene, Heterologous expression, genomic DNA, Computational biology, Genetics, Botany, Cyanobacteria, Recombinant DNA, Ecology, Bacteria, Raw material

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Cited by: 6

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