Protocol for using single-cell sequencing to study the heterogeneity of NF1 nerve sheath tumors from clinical biospecimens Article Swipe
Xiyuan Zhang
,
Vishaka Gopalan
,
Neeraja Syed
,
Sridhar Hannenhalli
,
Jack F. Shern
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2023.102297
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1016/j.xpro.2023.102297
Single-cell sequencing is a powerful technology to understand the heterogeneity of clinical biospecimens. Here, we present a protocol for obtaining single-cell suspension from neurofibromatosis type 1-associated nerve sheath tumors for transcriptomic profiling on the 10x platform. We describe steps for clinical sample collection, generation of single-cell suspension, and cell capture and sequencing. We then detail methods for integrative analysis, developmental Schwann cell trajectory building using bioinformatic tools, and comparative analysis. This protocol can be adapted for single-cell sequencing using mouse nerve tumors. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.xpro.2023.102297
- OA Status
- gold
- Cited By
- 3
- References
- 17
- Related Works
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- OpenAlex ID
- https://openalex.org/W4376120179
All OpenAlex metadata
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https://openalex.org/W4376120179Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.xpro.2023.102297Digital Object Identifier
- Title
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Protocol for using single-cell sequencing to study the heterogeneity of NF1 nerve sheath tumors from clinical biospecimensWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-05-10Full publication date if available
- Authors
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Xiyuan Zhang, Vishaka Gopalan, Neeraja Syed, Sridhar Hannenhalli, Jack F. ShernList of authors in order
- Landing page
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https://doi.org/10.1016/j.xpro.2023.102297Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
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https://doi.org/10.1016/j.xpro.2023.102297Direct OA link when available
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Schwann cell, Protocol (science), Neurofibromatosis, Computational biology, Computer science, Biology, Medicine, Pathology, Anatomy, Genetics, Alternative medicineTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2025: 3Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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