Pulse train gating to improve signal generation for in vivo two-photon fluorescence microscopy Article Swipe

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Shaun A. Engelmann , Alankrit Tomar , Aaron L. Woods , Andrew K. Dunn ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1117/1.nph.10.4.045006 · OA: W4388408050

We demonstrate that a pulse gating strategy can either be used to limit heating when imaging superficial brain regions or used to increase signal generation in deep regions. These findings should encourage others to adopt similar pulse gating excitation schemes for imaging neural structures through two-photon microscopy.