Quantification of the molar concentration of the NGS libraries by qPCR v1 Article Swipe

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Yoko Kato‐Unoki ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.17504/protocols.io.bmgck3sw

Using the NGS library concentration obtained from the Agilent BioAnalyzer, dilute the libraries to 100 pg/μl with 10 mM Tris-HCl, pH 8.0.Then prepare a 1:10000 dilution of each diluted library (final concentration, 0.01 pg/μl) by conducting 10-fold dilution series using 10 mM Tris-HCl, pH 8.0.*If the library concentration is unknown, conduct a 10-fold dilution series to achieve an appropriate final dilution (Note: for a 1:3 diluted library, we conducted a 1:500,000 dilution with 10 mM Tris-HCl, pH 8.0).

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Type: preprint
Language: en
Landing Page: https://doi.org/10.17504/protocols.io.bmgck3sw
PDF: https://www.protocols.io/view/quantification-of-the-molar-concentration-of-the-n-bmgck3sw.pdf
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DOI: https://doi.org/10.17504/protocols.io.bmgck3sw

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Title: Quantification of the molar concentration of the NGS libraries by qPCR v1

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Type: preprint

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Language: en

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Publication year: 2020

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Publication date: 2020-09-17

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Authors: Yoko Kato‐Unoki

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Landing page: https://doi.org/10.17504/protocols.io.bmgck3sw

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PDF URL: https://www.protocols.io/view/quantification-of-the-molar-concentration-of-the-n-bmgck3sw.pdf

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