Quantitative analysis of unconjugated and total bisphenol A in human urine using solid-phase extraction and UPLC–MS/MS: Method implementation, method qualification and troubleshooting Article Swipe
B.A.P. Buscher
,
Dick van de Lagemaat
,
W. Gries
,
D. Beyer
,
Dan A. Markham
,
Robert A. Budinsky
,
Stephen S. Dimond
,
Rajesh Vade Nath
,
Stephanie A. Snyder
,
Steven G. Hentges
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.1016/j.jchromb.2015.09.020
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.1016/j.jchromb.2015.09.020
The aim of the presented investigation was to document challenges encountered during implementation and qualification of a method for bisphenol A (BPA) analysis and to develop and discuss precautions taken to avoid and to monitor contamination with BPA during sample handling and analysis. Previously developed and published HPLC-MS/MS methods for the determination of unconjugated BPA (Markham et al. Journal of Analytical Toxicology, 34 (2010) 293-303) [17] and total BPA (Markham et al. Journal of Analytical Toxicology, 38 (2014) 194-203) [20] in human urine were combined and transferred into another laboratory. The initial method for unconjugated BPA was developed and evaluated in two independent laboratories simultaneously. The second method for total BPA was developed and evaluated in one of these laboratories to conserve resources. Accurate analysis of BPA at sub-ppb levels is a challenging task as BPA is a widely used material and is ubiquitous in the environment at trace concentrations. Propensity for contamination of biological samples with BPA is reported in the literature during sample collection, storage, and/or analysis. Contamination by trace levels of BPA is so pervasive that even with extraordinary care, it is difficult to completely exclude the introduction of BPA into biological samples and, consequently, contamination might have an impact on BPA biomonitoring data. The applied UPLC-MS/MS method was calibrated from 0.05 to 25ng/ml. The limit of quantification was 0.1ng/ml for unconjugated BPA and 0.2ng/ml for total BPA, respectively, in human urine. Finally, the method was applied to urine samples derived from 20 volunteers. Overall, BPA can be analyzed in human urine with acceptable recovery and repeatability if sufficient measures are taken to avoid contamination throughout the procedure from sample collection until UPLC-MS/MS analysis.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.jchromb.2015.09.020
- OA Status
- hybrid
- Cited By
- 17
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W1754977952
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W1754977952Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1016/j.jchromb.2015.09.020Digital Object Identifier
- Title
-
Quantitative analysis of unconjugated and total bisphenol A in human urine using solid-phase extraction and UPLC–MS/MS: Method implementation, method qualification and troubleshootingWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2015Year of publication
- Publication date
-
2015-09-27Full publication date if available
- Authors
-
B.A.P. Buscher, Dick van de Lagemaat, W. Gries, D. Beyer, Dan A. Markham, Robert A. Budinsky, Stephen S. Dimond, Rajesh Vade Nath, Stephanie A. Snyder, Steven G. HentgesList of authors in order
- Landing page
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https://doi.org/10.1016/j.jchromb.2015.09.020Publisher landing page
- Open access
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YesWhether a free full text is available
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-
hybridOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1016/j.jchromb.2015.09.020Direct OA link when available
- Concepts
-
Chemistry, Chromatography, Contamination, Bisphenol A, Solid phase extraction, Biomonitoring, Sample preparation, Urine, Troubleshooting, Extraction (chemistry), Detection limit, Environmental chemistry, Computer science, Biochemistry, Organic chemistry, Operating system, Epoxy, Ecology, BiologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
17Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3, 2022: 4, 2020: 4, 2019: 1, 2018: 2Per-year citation counts (last 5 years)
- References (count)
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25Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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