Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars Article Swipe

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Tozivepi Aaron Munyayi , Danielle Wingrove Mulder , Engela H. Conradie , Barend Christiaan Vorster ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.20944/preprints202310.0610.v1

We describe a competitive colorimetric assay that enables rapid and sensitive detection of galactose and reduced nicotinamide adenine dinucleotide (NADH) via colorimetric readouts and demon-strates its usefulness for monitoring NAD+-driven enzymatic reactions. We present a sensitive plasmonic sensing approach for assessing galactose concentration and the presence of NADH using galactose dehydrogenase immobilized gold nanostars (AuNS-PVP-GalDH). The AuNS-PVP-GalDH assay remains turquoise blue in the absence of galactose and NADH; however, as galactose and NADH concentrations grow, the reaction well color changes to a characteristic red color in the presence of an alkaline environment and metal ions catalyst (detection solution). As a result, when galactose is sensed in the presence of H2O2, the colored response of the AuNS-PVP-GalDH assay transforms from turquoise blue to light pink, then to wine red in a con-centration-dependent manner discernible to the human eye. This competitive AuNS-PVP-GalDH assay could be a viable analytical tool for rapid and convenient galactose quantification in re-source-limited areas.

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Galactose Chemistry NAD+ kinase Nicotinamide adenine dinucleotide Biochemistry Colorimetry Dehydrogenase Colloidal gold Chromatography Enzyme Nanotechnology Materials science Nanoparticle

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Type: preprint
Language: en
Landing Page: https://doi.org/10.20944/preprints202310.0610.v1
PDF: https://www.preprints.org/manuscript/202310.0610/v1/download
OA Status: green
Cited By: 1
References: 67
Related Works: 10
OpenAlex ID: https://openalex.org/W4387650542

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DOI: https://doi.org/10.20944/preprints202310.0610.v1

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Title: Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars

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Type: preprint

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Language: en

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Publication year: 2023

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Publication date: 2023-10-10

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Authors: Tozivepi Aaron Munyayi, Danielle Wingrove Mulder, Engela H. Conradie, Barend Christiaan Vorster

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Landing page: https://doi.org/10.20944/preprints202310.0610.v1

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PDF URL: https://www.preprints.org/manuscript/202310.0610/v1/download

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Concepts: Galactose, Chemistry, NAD+ kinase, Nicotinamide adenine dinucleotide, Biochemistry, Colorimetry, Dehydrogenase, Colloidal gold, Chromatography, Enzyme, Nanotechnology, Materials science, Nanoparticle

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Cited by: 1

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abstract_inverted_index.usefulness	26
abstract_inverted_index.NAD+-driven	29
abstract_inverted_index.competitive	3, 138
abstract_inverted_index.discernible	132
abstract_inverted_index.environment	91
abstract_inverted_index.immobilized	51
abstract_inverted_index.colorimetric	4, 21
abstract_inverted_index.dinucleotide	18
abstract_inverted_index.nicotinamide	16
abstract_inverted_index.concentration	42
abstract_inverted_index.dehydrogenase	50
abstract_inverted_index.demon-strates	24
abstract_inverted_index.AuNS-PVP-GalDH	56, 115, 139
abstract_inverted_index.characteristic	82
abstract_inverted_index.concentrations	73
abstract_inverted_index.quantification	152
abstract_inverted_index.(AuNS-PVP-GalDH).	54
abstract_inverted_index.re-source-limited	154
abstract_inverted_index.con-centration-dependent	130
cited_by_percentile_year.max	94
cited_by_percentile_year.min	89
countries_distinct_count	1
institutions_distinct_count	4
citation_normalized_percentile.value	0.52208238
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False