Rapid identification of A29L antibodies based on mRNA immunization and high-throughput single B cell sequencing to detect Monkeypox virus Article Swipe
Huisheng Sun
,
Yiqi Miao
,
Xingsheng Yang
,
Liang Guo
,
Qingyu Li
,
Jing Wang
,
Jinrong Long
,
Zhen Zhang
,
Jingqi Shi
,
Jian Li
,
Yiming Cao
,
Changxiao Yu
,
Jierui Mai
,
Zhen Rong
,
Jiannan Feng
,
Shumei Wang
,
Jing Yang
,
Shengqi Wang
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1080/22221751.2024.2332665
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1080/22221751.2024.2332665
With the large number of atypical cases in the mpox outbreak, which was classified as a global health emergency by the World Health Organization (WHO) on 23 July 2022, rapid diagnosis of mpox and diseases with similar symptoms to mpox such as chickenpox and respiratory infectious diseases in the early stages of viral infection is key to controlling the spread of the outbreak. In this study, antibodies against the monkeypox virus A29L protein were efficiently and rapidly identified by combining rapid mRNA immunization with high-throughput sequencing of individual B cells. We obtained eight antibodies with a high affinity for A29L validated by ELISA, which were was used as the basis for developing an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobeads (SiTQD-ICA). The SiTQD-ICA biosensor utilizing M53 and M78 antibodies showed high sensitivity and stability of detection: A29L was detected within 20 min, with a minimum detection limit of 5 pg/mL. A specificity test showed that the method was non-cross-reactive with chickenpox or common respiratory pathogens and can be used for early and rapid diagnosis of monkeypox virus infection by antigen detection. This antibody identification method can also be used for rapid acquisition of monoclonal antibodies in early outbreaks of other infectious diseases for various studies.
Related Topics
Concepts
Virology
Monkeypox
Outbreak
Antibody
Virus
Monoclonal antibody
Biology
Immunology
Gene
Vaccinia
Recombinant DNA
Biochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1080/22221751.2024.2332665
- https://www.tandfonline.com/doi/pdf/10.1080/22221751.2024.2332665?needAccess=true
- OA Status
- gold
- Cited By
- 11
- References
- 36
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4393067139
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4393067139Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1080/22221751.2024.2332665Digital Object Identifier
- Title
-
Rapid identification of A29L antibodies based on mRNA immunization and high-throughput single B cell sequencing to detect Monkeypox virusWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-03-22Full publication date if available
- Authors
-
Huisheng Sun, Yiqi Miao, Xingsheng Yang, Liang Guo, Qingyu Li, Jing Wang, Jinrong Long, Zhen Zhang, Jingqi Shi, Jian Li, Yiming Cao, Changxiao Yu, Jierui Mai, Zhen Rong, Jiannan Feng, Shumei Wang, Jing Yang, Shengqi WangList of authors in order
- Landing page
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https://doi.org/10.1080/22221751.2024.2332665Publisher landing page
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https://www.tandfonline.com/doi/pdf/10.1080/22221751.2024.2332665?needAccess=trueDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
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-
goldOpen access status per OpenAlex
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https://www.tandfonline.com/doi/pdf/10.1080/22221751.2024.2332665?needAccess=trueDirect OA link when available
- Concepts
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Virology, Monkeypox, Outbreak, Antibody, Virus, Monoclonal antibody, Biology, Immunology, Gene, Vaccinia, Recombinant DNA, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
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11Total citation count in OpenAlex
- Citations by year (recent)
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2025: 7, 2024: 4Per-year citation counts (last 5 years)
- References (count)
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36Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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