Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method Article Swipe
Chao Li
,
Gongyu Fu
,
Yaoqiang Shi
,
A‐Mei Zhang
,
Xueshan Xia
,
Yue Fang
,
Xiaoqin Mao
,
Jie Jiang
,
Yuzhu Song
,
Guangying Yang
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1590/1414-431x20198186
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1590/1414-431x20198186
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed that the LAMP targeted to ureR_1 is a fast, specific, sensitive, inexpensive, and suitable method for the detection of K. pneumoniae.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1590/1414-431x20198186
- http://www.scielo.br/pdf/bjmbr/v52n3/1414-431X-bjmbr-52-3-e8186.pdf
- OA Status
- gold
- Cited By
- 5
- References
- 35
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2924434016
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2924434016Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1590/1414-431x20198186Digital Object Identifier
- Title
-
Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification methodWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2019Year of publication
- Publication date
-
2019-01-01Full publication date if available
- Authors
-
Chao Li, Gongyu Fu, Yaoqiang Shi, A‐Mei Zhang, Xueshan Xia, Yue Fang, Xiaoqin Mao, Jie Jiang, Yuzhu Song, Guangying YangList of authors in order
- Landing page
-
https://doi.org/10.1590/1414-431x20198186Publisher landing page
- PDF URL
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https://www.scielo.br/pdf/bjmbr/v52n3/1414-431X-bjmbr-52-3-e8186.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.scielo.br/pdf/bjmbr/v52n3/1414-431X-bjmbr-52-3-e8186.pdfDirect OA link when available
- Concepts
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Loop-mediated isothermal amplification, Klebsiella pneumoniae, Microbiology, Bacteria, Plasmid, Pneumonia, Pathogenic bacteria, Gene, Biology, Sepsis, Medicine, Escherichia coli, Immunology, DNA, Genetics, Internal medicineTop concepts (fields/topics) attached by OpenAlex
- Cited by
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5Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2023: 2, 2022: 1, 2021: 1Per-year citation counts (last 5 years)
- References (count)
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35Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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