Real-time visualization of STAT activation in live cells using genetically encoded biosensors Article Swipe
Thu Annelise Nguyen
,
Roman Meledin
,
Karin J. Seubert-Buholzer
,
Flurin Sturzenegger
,
Urs Ziegler
,
Ufuk Karakus
,
Onur Boyman
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1038/s41589-025-02012-0
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1038/s41589-025-02012-0
Signal transducer and activator of transcription (STAT) is a family of key transcriptional regulators in immune, epithelial and mesenchymal cells. Aberrant STAT activity is associated with malignancy, autoimmunity and immunodeficiency. The STAT signaling pathways are very attractive drug targets; however, validated tools to monitor real-time activation of STATs are lacking. Here, we developed a class of highly sensitive genetically encoded STAT biosensors, termed STATeLights, which allowed direct and continuous detection of STAT activity in live cells with high spatiotemporal resolution. Using human STAT5A, we demonstrate the versatility of STATeLight5A to quantify the activation of wild-type STAT5 versus disease-associated STAT5 mutants and to precisely select compounds targeting the STAT5 signaling pathway. Moreover, STATeLight5A also facilitated real-time tracking of STAT5 activation in human primary CD4 + T cells. Collectively, our biosensors open up unprecedented possibilities of studying STAT biology and druggability in various cellular contexts.
Related Topics
Concepts
stat
STAT protein
STAT5
STAT4
Biology
Druggability
Cell biology
Signal transduction
JAK-STAT signaling pathway
Computational biology
Genetics
Gene
Receptor tyrosine kinase
STAT3
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1038/s41589-025-02012-0
- https://www.nature.com/articles/s41589-025-02012-0.pdf
- OA Status
- hybrid
- Cited By
- 1
- References
- 50
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4413874638
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4413874638Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1038/s41589-025-02012-0Digital Object Identifier
- Title
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Real-time visualization of STAT activation in live cells using genetically encoded biosensorsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-09-01Full publication date if available
- Authors
-
Thu Annelise Nguyen, Roman Meledin, Karin J. Seubert-Buholzer, Flurin Sturzenegger, Urs Ziegler, Ufuk Karakus, Onur BoymanList of authors in order
- Landing page
-
https://doi.org/10.1038/s41589-025-02012-0Publisher landing page
- PDF URL
-
https://www.nature.com/articles/s41589-025-02012-0.pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
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https://www.nature.com/articles/s41589-025-02012-0.pdfDirect OA link when available
- Concepts
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stat, STAT protein, STAT5, STAT4, Biology, Druggability, Cell biology, Signal transduction, JAK-STAT signaling pathway, Computational biology, Genetics, Gene, Receptor tyrosine kinase, STAT3Top concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1Per-year citation counts (last 5 years)
- References (count)
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50Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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