Recent Developments in the Application of Flow Cytometry to Advance our Understanding of Mycobacterium tuberculosis Physiology and Pathogenesis
Article Swipe
Trisha Parbhoo
,
Samantha L. Sampson
,
Jomien Mouton
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1002/cyto.a.24030
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1002/cyto.a.24030
The ability of the bacterial pathogen Mycobacterium tuberculosis to adapt and survive within human cells to disseminate to other individuals and cause active disease is poorly understood. Research supports that as M. tuberculosis adapts to stressors encountered in the host, it exhibits variable physiological and metabolic states that are time and niche‐dependent. Challenges associated with effective treatment and eradication of tuberculosis (TB) are in part attributed to our lack of understanding of these different mycobacterial phenotypes. This is mainly due to a lack of suitable tools to effectively identify/detect heterogeneous bacterial populations, which may include small, difficult‐to‐culture subpopulations. Importantly, flow cytometry allows rapid and affordable multiparametric measurements of physical and chemical characteristics of single cells, without the need to preculture cells. Here, we summarize current knowledge of flow cytometry applications that have advanced our understanding of the physiology of M. tuberculosis during TB disease. Specifically, we review how host‐associated stressors influence bacterial characteristics such as metabolic activity, membrane potential, redox status and the mycobacterial cell wall. Further, we highlight that flow cytometry offers unprecedented opportunities for insight into bacterial population heterogeneity, which is increasingly appreciated as an important determinant of disease outcome. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
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Metadata
- Type
- review
- Language
- en
- Landing Page
- https://doi.org/10.1002/cyto.a.24030
- OA Status
- hybrid
- Cited By
- 17
- References
- 120
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3028569326
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3028569326Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/cyto.a.24030Digital Object Identifier
- Title
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Recent Developments in the Application of Flow Cytometry to Advance our Understanding of
Mycobacterium tuberculosis Physiology and PathogenesisWork title - Type
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reviewOpenAlex work type
- Language
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enPrimary language
- Publication year
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2020Year of publication
- Publication date
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2020-05-21Full publication date if available
- Authors
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Trisha Parbhoo, Samantha L. Sampson, Jomien MoutonList of authors in order
- Landing page
-
https://doi.org/10.1002/cyto.a.24030Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1002/cyto.a.24030Direct OA link when available
- Concepts
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Flow cytometry, Mycobacterium tuberculosis, Tuberculosis, Population, Biology, Cytometry, Disease, Immunology, Phenotype, Computational biology, Medicine, Pathology, Genetics, Environmental health, GeneTop concepts (fields/topics) attached by OpenAlex
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17Total citation count in OpenAlex
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2025: 2, 2024: 3, 2023: 3, 2022: 5, 2021: 2Per-year citation counts (last 5 years)
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120Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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