L-mCherry-lacZ viruses. Article Swipe

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mCherry lac operon Biology Cell biology Molecular biology Chemistry Virology Genetics Gene Recombinant DNA Green fluorescent protein

Paszkowski Patrick , S. Noyce Ryan , Evans David H. ·

YOU? · · 2016 · Open Access · · DOI: https://doi.org/10.1371/journal.ppat.1005824.g009 · OA: W2518019543

<p>(A) The figure shows the two parent viruses, the predicted recombinants, and the <i>Hin</i>dIII fragments that should be detected by the pE/L oligonucleotide probe (blue bar). (B) Southern blot analysis of cells co-infected with the pE/L-mCherry(t) and pE/L-mCherry-lacZ viruses. BSC-40 cells were infected with each of the parental viruses at MOI = 5, or co-infected with the two viruses at a combined MOI = 5, and the DNA was extracted 24 h post-infection. The samples were then Southern blotted using a biotin-labeled probe. Although faint, two bands at 2.2 and 0.9 kbp are seen that indicate the presence of recombinant genomes. Collectively they comprise about 2% of the DNA.</p>