Reliable Approach for Pure Yeast Cell Wall Protein Isolation from Saccharomyces cerevisiae Yeast Cells Article Swipe
Marie Yammine
,
Fabrice Bray
,
Stéphanie Flament
,
Antoine Picavet
,
Jean-Marie Lacroix
,
Emmanuel Poilpré
,
Isabelle Mouly
,
Christian Rolando
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1021/acsomega.2c02176
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.1021/acsomega.2c02176
Saccharomyces cerevisiae yeast is a fungus presenting a peripheral organelle called the cell wall. The cell wall protects the yeast cell from stress and provides means for communication with the surrounding environment. It has a complex molecular structure, composed of an internal part of cross-linked polysaccharides and an external part of mannoproteins. These latter are very interesting owing to their functional properties, dependent on their molecular features with massive mannosylations. Therefore, the molecular characterization of mannoproteins is a must relying on the optimal isolation and preparation of the cell wall fraction. Multiple methods are well reported for yeast cell wall isolation. The most applied one consists of yeast cell lysis by mechanical disruption. However, applying this classical approach to S288C yeast cells showed considerable contamination with noncell wall proteins, mainly comprising mitochondrial proteins. Herein, we tried to further purify the yeast cell wall preparation by two means: ultracentrifugation and Triton X-100 addition. While the first strategy showed limited outcomes in mitochondrial protein removal, the second strategy showed optimal results when Triton X-100 was added at 5%, allowing the identification of more mannoproteins and significantly enriching their amounts. This promising method could be reliably implemented on the lab scale for identification of mannoproteins and molecular characterization and industrial processes for "pure" cell wall isolation.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acsomega.2c02176
- OA Status
- gold
- Cited By
- 12
- References
- 65
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4291618065
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4291618065Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/acsomega.2c02176Digital Object Identifier
- Title
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Reliable Approach for Pure Yeast Cell Wall Protein Isolation from Saccharomyces cerevisiae Yeast CellsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2022Year of publication
- Publication date
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2022-08-15Full publication date if available
- Authors
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Marie Yammine, Fabrice Bray, Stéphanie Flament, Antoine Picavet, Jean-Marie Lacroix, Emmanuel Poilpré, Isabelle Mouly, Christian RolandoList of authors in order
- Landing page
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https://doi.org/10.1021/acsomega.2c02176Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1021/acsomega.2c02176Direct OA link when available
- Concepts
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Yeast, Cell wall, Saccharomyces cerevisiae, Lysis, Biochemistry, Organelle, Cell, Biology, PolysaccharideTop concepts (fields/topics) attached by OpenAlex
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12Total citation count in OpenAlex
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2025: 4, 2024: 6, 2023: 1, 2022: 1Per-year citation counts (last 5 years)
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65Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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